Serum-free culture medium of placental mesenchymal stem cells

Abstract

The invention relates to the technical field of stem cells, in particular to a serum-free culture medium of placental mesenchymal stem cells. The serum-free culture medium of the placental mesenchymalstem cells comprises a DMEM low-sugar culture medium and a supplement, wherein the supplement comprises the following components: human serum albumin with a concentration of 1-5 mg / mL, transferrin with a concentration of 5-10 [mu]g / mL, an epidermal growth factor with a concentration of 5-10 ng / mL, a transforming growth factor with a concentration of 10-30 ng / mL, a platelet-derived growth factor with a concentration of 10-25 ng / mL, glutathione with a concentration of 1-5 [mu]g / mL, Pluronic F68 with a concentration of 10-20 [mu]g / mL and lignans with a concentration of 5-10 [mu]g / mL. The produced serum-free culture medium can significantly improve the proliferation capability of the placental mesenchymal stem cells, a survival rate of the placental mesenchymal stem cells is high, the culturecost is low, the culture time of the cells is shortened, the application range is wide, and the serum-free culture medium of the placental mesenchymal stem cells can be used for industrial production.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a serum-free culture medium for placental mesenchymal stem cells. Background technique [0002] Placental mesenchymal stem cells (MSCs) are a kind of pluripotent stem cells, pluripotent cells with strong proliferation ability and multilineage differentiation potential. MSC has the ability to differentiate into various cells such as epithelial stem cells, neural stem cells, liver stem cells, muscle cells, osteoblasts, chondrocytes, and stromal cells under suitable in vivo or in vitro environments. MSC has an immunoregulatory effect. Through the negative immune regulatory function, it can inhibit the body's hyperactive immune response and restore the balance of the body's immune function, so it can be used to treat immune rejection after hematopoietic stem cell transplantation and Crohn's disease, lupus erythematosus, and scleroderma. diseases and other autoimmune diseases. MS...

AI Technical Summary

Problems solved by technology

[0003] The traditional culture method of mesenchymal stem cells is to use culture medium containing animal serum, mainly to provide nutrients such as hormones, growth factors, transport proteins, etc. for the growth and reproduction of mesenchymal stem cells. However, serum is a very complex There may be as many as several hundred ingredients, and the exact ingredients, content and mechanism of action are still unclear. The quality of serum varies greatly between batches, which is greatly affected by the source of raw materials of the manufacturer. It is very difficult to ensure the consistency of each batch, which is not conducive to the standardization of laboratory research or product production. The serum may contain some unknown or undetected harmful microorganisms such as mycoplasma and viruses, which may have a potential impact on cells. Some substances such as antibodies, complements and bacterial toxins also have adverse effects on cell growth and even cause cell death

[0004] Chinese patent application CN201911219419.5 discloses a serum-free medium and its preparation method. The medium includes non-essential amino acids, glutamine, hydrocortisone, dexamethasone, polyvinyl alcohol, recombinant human insulin, recombinant human Transferrin, recombinant human epidermal growth factor, recombinant basic fibroblast growth factor, etc., the medium includes more than 20 components, the cost is expensive, and the serum-free medium is only suitable for a certain type of cell culture, suitable for The range is small, therefore, there is room for improvement