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Preparation method of cartilage repair scaffold material

A scaffold material and cartilage repair technology, applied in medical science, prosthesis, surgery, etc., can solve the problems of poor performance of gas permeable cell proliferation and growth, and achieve favorable growth and proliferation, rapid proliferation, and large specific surface area Effect

Inactive Publication Date: 2014-04-02
李克尊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current natural scaffold materials mainly process natural polymers and other substances into hydrogels as cartilage tissue repair scaffolds, but the cartilage repair scaffolds made of hydrogels have poor air permeability and poor cell proliferation and growth performance.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method for cartilage repair support material, comprising the following steps:

[0022] Preparation mass concentration is the acetic acid aqueous solution of 1wt% chitosan, the mass concentration of described acetic acid aqueous solution is 5wt%, it is fully stirred, obtains uniform transparent chitosan solution;

[0023] Adding nano-hydroxyapatite to the chitosan solution to make its mass concentration 10wt%, fully stirring it to obtain a uniform and transparent chitosan solution of hydroxyapatite;

[0024] Prepare an aqueous solution with a mass concentration of 10wt% PVP, and fully stir it to obtain a uniform and transparent aqueous solution of water-soluble synthetic polymers;

[0025] The chitosan solution containing hydroxyapatite and the aqueous solution of PVP are mixed uniformly in a ratio of 5:1 by volume;

[0026] Add the mixed solution to the solution supply device, set the spinning voltage to 10kV, the spinning distance between the spinning po...

Embodiment 2

[0030] A preparation method for cartilage repair support material, comprising the following steps:

[0031] Preparation mass concentration is the acetic acid aqueous solution of 10wt% chitosan, the mass concentration of described acetic acid aqueous solution is 1wt%, it is fully stirred, obtains uniform transparent chitosan solution;

[0032] Adding nano-hydroxyapatite to the chitosan solution to make its mass concentration 25 wt%, fully stirring it to obtain a uniform and transparent chitosan solution of hydroxyapatite;

[0033] Prepare an aqueous solution with a mass concentration of 1 wt% PEO, and stir it fully to obtain a uniform and transparent aqueous solution of water-soluble synthetic polymers;

[0034] The chitosan solution containing hydroxyapatite and the aqueous solution of PEO are mixed uniformly in a ratio of 9:1 by volume;

[0035] Add the mixed solution to the solution supply device, set the spinning voltage to 20kV, the spinning distance between the spinning ...

Embodiment 3

[0039] A preparation method for cartilage repair support material, comprising the following steps:

[0040] Preparation mass concentration is the acetic acid aqueous solution of 4wt% chitosan, the mass concentration of described acetic acid aqueous solution is 4wt%, it is fully stirred, obtains uniform transparent chitosan solution;

[0041] Adding nano-hydroxyapatite to the chitosan solution to make its mass concentration 15 wt%, fully stirring it to obtain a uniform and transparent chitosan solution of hydroxyapatite;

[0042] Prepare an aqueous solution with a mass concentration of 3wt% PEG, and fully stir it to obtain a uniform and transparent aqueous solution of water-soluble synthetic polymers;

[0043] Mix the chitosan solution containing hydroxyapatite and the aqueous solution of PEG in a volume ratio of 7:1;

[0044] Add the mixed solution to the solution supply device, set the spinning voltage to 16kV, the spinning distance between the spinning port and the receivin...

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Abstract

The invention relates to the technical field of tissue engineering scaffolds, and in particular relates to a preparation method of a cartilage repair scaffold material. The preparation method comprises the following steps: firstly, preparing chitosan nano fibers containing hydroxyapatite by virtue of electrostatic spinning; then, packing by using hyaluronic acid; finally, preparing the cartilage repair scaffold material by virtue of a freeze-drying experimental method. The cartilage repair scaffold material obtained by the preparation method disclosed by the invention has a relatively large specific area and is favorable for cartilage cells to grow and proliferate; hydroxyapatite serves as the main component in a human bone to provide a lot of necessary raw materials for the growth of the cartilage cells; hyaluronic acid is the main component of cartilage liquid and is favorable for providing a good growth environment for the cartilage cells; meanwhile, the hyaluronic acid can be compounded with chitosan to package nano hydroxyapatite in chitosan so as to form a sustained-release system of hydroxyapatite; growth factors can be used for promoting rapid proliferation of the cartilage cells.

Description

technical field [0001] The invention relates to the technical field of tissue engineering scaffolds, in particular to a method for preparing a scaffold material for cartilage repair. Background technique [0002] Articular cartilage is a relatively special tissue in the human body. It has no blood vessels, nerves and lymphocytes, so its self-repair ability is very poor. At present, the traditional clinical treatment methods mainly include microfracture, subchondral bone drilling, subchondral grinding, autologous cartilage transplantation and autologous cell transplantation. secondary trauma. At present, there is no method that can successfully replace the damaged site and achieve the purpose of cartilage regeneration. [0003] Currently used cartilage repair scaffold materials can be roughly divided into two categories: natural scaffold materials and artificial scaffold materials. Natural scaffold materials are macromolecular materials that are derived from animals, plant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/12A61L27/20A61L27/54A61L27/18A61L27/16A61L31/02A61L31/04A61L31/06A61L31/16
Inventor 李克尊张春红郭占东李晓辉
Owner 李克尊
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