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Exosome hydrogel wound dressing and preparation method thereof

A wound dressing and exosome technology, applied in the field of biomedicine, can solve problems such as uneven mixing of hydrogel and active ingredients, affecting the effect of active ingredients, and failure to achieve sustained release, so as to induce epithelial cell proliferation and vascular The effect of forming, promoting skin wound healing, and accelerating wound healing

Pending Publication Date: 2020-07-24
JILIN UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although traditional hydrogels such as chitosan and silver ion dressings have good sterilization and antibacterial effects, they all have certain toxicity, have certain damage to skin and soft tissues, and affect the effect of wound healing.
In addition, wound dressing hydrogels are generally colloidal solid substances, which are prepared from complex and high-cost biomolecules through complex reactions. Generally, cross-linking is required, and the configuration method is complicated, and it is easy for the hydrogel to mix with active ingredients. Disadvantages of inhomogeneity, failure to achieve sustained release, and affecting the effect of active ingredients, so the existing dressings prepared from hydrogels and endosomes still have the disadvantage of short healing time

Method used

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  • Exosome hydrogel wound dressing and preparation method thereof
  • Exosome hydrogel wound dressing and preparation method thereof
  • Exosome hydrogel wound dressing and preparation method thereof

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preparation example Construction

[0031] The present invention also provides a method for preparing an exosome hydrogel wound dressing, comprising the following steps: preparing physiological salts with a concentration of 400-440 mg / mL Pluronic F127 and a concentration of 50-200 mg / mL Pluronic F68 aqueous solution to obtain liquid A; prepare a resuspension of mesenchymal stem cell exosomes in physiological saline with a concentration of 200-400 μg / mL to obtain liquid B; mix liquid A with the liquid B at 4°C to obtain exosomes Body hydrogel wound dressing.

[0032] At present, wound dressing hydrogels are generally solid substances, which generally require cross-linking reactions. The configuration method is complicated, and it is easy to have the disadvantages of uneven mixing of hydrogel and active ingredients, failure to achieve sustained release, and affecting the effect of active ingredients. . However, the hydrogel used in the present invention is a temperature-sensitive hydrogel, which is in a liquid st...

Embodiment 1

[0042] Prepare a physiological saline solution with a concentration of 440 mg / mL of Pluronic F127 and a concentration of 100 mg / mL of Pluronic F68 to obtain solution A; the supernatant of the mesenchymal stem cell culture medium was statically incubated at 4°C with PEG6000. Place overnight, centrifuge, and resuspend the obtained pellet with PBS to obtain mesenchymal stem cell exosomes. Prepare a suspension of mesenchymal stem cell exosomes in physiological saline with a concentration of 400 μg / mL to obtain liquid B; mix liquid A with the liquid B at a volume ratio of 1:1 at 4°C to obtain exosome water Gel wound dressing.

Embodiment 2

[0044] Prepare a physiological saline solution with a concentration of 440 mg / mL of Pluronic F127 and a concentration of 50 mg / mL of Pluronic F68 to obtain solution A; the supernatant of the mesenchymal stem cell culture medium was statically incubated at 4°C with PEG6000. Place overnight, centrifuge, and resuspend the obtained pellet with PBS to obtain mesenchymal stem cell exosomes. Prepare a suspension of mesenchymal stem cell exosomes in physiological saline with a concentration of 200 μg / mL to obtain liquid B; mix liquid A with the liquid B at a volume ratio of 1:1 at 4°C to obtain exosome water Gel wound dressing.

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Abstract

The invention belongs to the technical field of biomedicine, and particularly relates to an exosome hydrogel wound dressing and a preparation method thereof. The exosome hydrogel wound dressing comprises mesenchymal stem cell exosomes and hydrogel, and the hydrogel is composed of pluronic F127 and pluronic F68. The preparation method comprises the following steps: respectively preparing physiological saline solutions of pluronic F127 and pluronic F68 and mesenchymal stem cell exosome physiological saline resuspension, and mixing the solutions at 4 DEG C to obtain the exosome hydrogel wound dressing. Experimental results show that compared with the mode that mesenchymal stem cell exosomes and hydrogel are independently adopted, the wound dressing prepared through the method can better promote regeneration of skin wound cells and shorten the wound healing time.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an exosome hydrogel wound dressing and a preparation method thereof. Background technique [0002] According to statistics, more than 2% of people in my country experience the pain of chronic wound healing, and the annual cost of wound care products worldwide is as high as 15 billion US dollars. Traditional wound dressings such as chitosan, hyaluronic acid, alginate, and gelatin have certain adjuvant therapeutic effects on chronic wounds, but the degree of wound healing still mainly depends on the patient's self-healing ability, resulting in a large number of chronic refractory wounds. Patients with poor nutritional status, advanced age, low immunity and other reasons and long-term persistent and even lead to death. Therefore, there is an urgent need to develop new dressings with tissue regeneration functions based on clinical wound care needs. [0003] Mesenchy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/0019A61L26/0057A61L26/0066A61L26/008A61L2300/30A61L2300/412C08L71/02
Inventor 王立生聂文博赵丽晶吴祖泽
Owner JILIN UNIV
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