7results about How to "Promotes re-epithelialization" patented technology

The invention discloses high-strength gelatin / carbonic acid dihydrazide modified sodium alginate natural polymer hydrogel for 3D printing and a preparation method and application of the high-strength gelatin / carbonic acid dihydrazide modified sodium alginate natural polymer hydrogel. A gelatin solution and a carbonic acid dihydrazide modified sodium alginate solution are blended to obtain gelatin / carbonic acid dihydrazide modified sodium alginate ink; then, the hydrogel subjected to ink 3D printing is sequentially soaked in a CaCl2 solution and soaked in an EDC / NHS solution for cross-linking to be subjected to sequential enhancement treatment, and the high-strength natural polymer hydrogel is obtained. In addition, bioactive factors can be introduced into the 3D printing high-strength natural polymer hydrogel system, so that a bioactive hydrogel stent meeting clinical requirements is prepared. The biological ink A loaded with the growth factor for promoting corneal epithelialization and the biological ink B loaded with the histone deacetylase inhibitor are printed layer by layer through a multi-nozzle 3D printing system, so that the bioactive double-layer hydrogel scaffold can be obtained and used for corneal regeneration. The operation involved in the preparation process of the 3D printing high-strength natural polymer hydrogel is simple and convenient, and the system has high designability and universality.

The invention relates to the technical field of biology, in particular to an inducer for promoting exosome secretion and application of an exosome. The inducer comprises the following three components: N-methyldopamine hydrochloride, quercetin and ascorbic acid, has the advantages of stable effect and simplicity in use, can increase the yield of exosomes by at least 3-5 times when the umbilical cord mesenchymal stem cells in a good state are subjected to induction culture, and has a remarkable effect of promoting the secretion of the exosomes in the stem cells. In addition, the engineering exosome which is derived from the umbilical cord mesenchymal stem cells and simultaneously contains miR-125b-5p, miR-126-3p, miR-146a and miR-542-3p is prepared through an electroporation transfection technology, animal experiment results show that the engineering exosome has a wound repairing effect, and the comprehensive application of an inducer and an electrotransformation technology has relatively high application value; popularization and application are facilitated.

The invention discloses hydrogel as well as a preparation method and application thereof, belongs to the technical field of medical materials, and solves the problems of high recurrence rate and low curative effect of a treatment strategy of a diabetic wound in long-term treatment in the prior art. The method comprises the following steps: mixing a glutaminase inhibitor and a nano drug loading system, centrifuging, and collecting the precipitate to obtain a glutaminase inhibitor carrier, the drug loading system having oxidation resistance and drug loading capacity; mixing the modified hyaluronic acid with a gelatin solution to obtain gel; and adding the glutaminase inhibitor and the carrier into the gel, co-stirring, and adjusting the pH value to 7-8 to obtain the hydrogel. The hydrogel provided by the invention realizes specific removal of senescent cells from the source and remodeling of the ecological niche of the wound surface at the same time, so as to promote reepithelization and collagen deposition of diabetic tissues.

The application discloses a double-sided hydrogel scaffold for urethral repair and a preparation process thereof, and belongs to the technical field of biomaterials. The PCH@PVA scaffold is constructed, a base layer on the tissue side creates a eutrophication and guiding microenvironment for regeneration by using heparin, and a functional layer on the lumen side effectively blocks the penetration of harmful components in urine and the colonization of bacteria by a polyvinyl alcohol coating, and by optimizing the crosslinking degree of PVA, the PVA has excellent mechanical toughness and elasticity, can not only withstand the periodic expansion of the urethra without breaking, but also can transmit mechanical stimulation close to the physiological range, guide cells to functional regeneration instead of pathological fibrosis. The PCH@PVA scaffold provided by the application has excellent effects in promoting re-epithelialization, angiogenesis, inhibiting fibrosis and preventing stenosis. The application also provides a new design paradigm and theoretical basis for tissue regeneration strategies in other complex physiological microenvironments.

The invention discloses a CeO2 nano-enzyme loaded SilMA hydrogel and application thereof in a 3D biological printing tissue engineering trachea, and belongs to the technical field of biology.The preparation method of the CeO2 nano-enzyme loaded SilMA hydrogel comprises the steps that 1, CeO2 nano-enzyme is prepared, specifically, cerous nitrate hexahydrate, dextran and deionized water are stirred to be uniform, ammonia water is added, water bath heating is conducted, and the CeO2 nano-enzyme is prepared; carrying out aging, centrifugation, washing, vacuum drying and calcination on the obtained CeO2 nano-enzyme precipitation slurry to obtain CeO2 nano-enzyme powder; step 2, mixing CeO2 nano-enzyme into a 20 w / v% SilMA solution, so as to obtain a CeO2 nano-enzyme loaded SilMA hydrogel; step 3, taking 20% w / v of SilMA as a water phase and a paraffin oil solution containing 5% w / v of Span 80 as an oil phase, mixing, stirring under water bath heating to obtain SilMA hydrogel microspheres, and freeze-drying to obtain a porous structure; the preparation method comprises the following steps: adding CeO2 nano-enzyme into deionized water containing the SilMA hydrogel microspheres, so as to obtain a SilMA hydrogel microsphere solution loaded with the CeO2 nano-enzyme;

The invention discloses an intelligent multi-mode bionic interactive wound repair device and a preparation method thereof, and relates to the technical field of medical instruments. The device comprises a high-adaptability flexible structure, a multi-stage gel dressing layer and a tearable anti-sticking layer. The high-adaptability flexible structure is made of a flexible material, crossed gully structures are designed on the two sides, and the high-adaptability flexible structure accords with ergonomics, surface laser engraving and a device framework capable of being connected, fixed and protected; the multi-stage gel dressing layer is composed of bionic three-layer hydrogel embedded with different sensors and indicators, and the top layer achieves bacteriostasis, forms a micro-magnetic field, promotes absorption and achieves synergistic repair at the same time; medicine carrying microspheres are embedded in the middle layer, medicine is controlled in a controlled mode according to needs, ultrasonic magnetic field interaction quick release is used for the acute stage, and non-intervention slow release is used for the middle-later stage; the bottom layer has double antibacterial effects and can be seamlessly adhered to the wound skin and non-invasively separated from the wound skin; the early wound infection warning indicator can recognize, integrate and feed back wound information, and functional treatment is achieved.

The present invention discloses an intelligent multi-modal bionic interactive wound repair device and a preparation method thereof, and relates to the technical field of medical devices. The device includes a highly adaptable flexible structure, a multi-level gel dressing layer, and a tearable anti-adhesive layer. The highly adaptable flexible structure is made of a flexible material, and is designed with a cross-groove structure on both sides, which is ergonomic, laser-engraved on the surface, and can connect, fix and protect the device architecture; the multi-level gel dressing layer is composed of a bionic three-layer hydrogel embedded with different sensors and indicators, wherein the top layer achieves antibacterial while forming a micromagnetic field to promote absorption and synergistic repair; the middle layer is embedded with drug-loaded microspheres, and the drug is released on demand, and the ultrasonic magnetic field interaction is used for the acute phase, and the sustained release is used for the middle and late phases when there is no intervention; the bottom layer is dual antibacterial, and can be seamlessly adhered to and non-invasively separated from the wound skin; the early infection alarm of the wound can identify, integrate and feedback wound information to achieve functional treatment.