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Formula of degreasing liquid in IgY preparation process and preparation method of formula

A preparation method and formula technology, which are applied in the preparation methods of peptides, chemical instruments and methods, and immunoglobulin from eggs, etc., can solve the problem of time-consuming, dangerous reagents affecting cost control or application scope, and must be centrifuged or reacted at low temperature. Steps and other issues to achieve a reasonable design effect

Inactive Publication Date: 2016-05-04
NORTHWEST A & F UNIV
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  • Abstract
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Problems solved by technology

In the process of industrial production of IgY, the traditional method has problems such as high price of extraction reagents, necessary centrifugation or low-temperature reaction steps, time-consuming and dangerous reagents, etc., which affect cost control or application range.
Similarly, the existing single-type lipid-removing formula is difficult to remove most of the lipids under the premise of controlling economic costs to obtain antibodies with higher purity

Method used

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  • Formula of degreasing liquid in IgY preparation process and preparation method of formula

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Embodiment

[0025] A kind of formula of the degreasing liquid in the process of preparing IgY, formula comprises the raw material of following weight and ratio: 3 parts of PluronicF68, 7.5 parts of PEG6000 dry powder, 1.5 parts of CaCl 2 , 0.8 parts of CH 3 COONa, the balance is double distilled water.

[0026] The present invention also provides a method for preparing the formula of the degreasing liquid in the process of preparing IgY, and the configuration process is as follows;

[0027] (1) Weigh 3g of food-grade Pluronic F68 into a 200ml blue silk-top bottle.

[0028] (2) Weigh 7.5g PEG6000 into the bottle.

[0029] (3) Weigh 1.5g CaCl 2 Add to bottle.

[0030] (4) Weigh 0.85g of sodium acetate into the bottle, add 100ml of double distilled water, mix well to form a clear and transparent egg yolk degreasing solution, and store at room temperature.

[0031] (5) Select immunized eggs, separate egg white and egg yolk, collect egg yolk liquid and dilute it with PBS 1:4 to make egg y...

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Abstract

The invention discloses a formula of degreasing liquid in an IgY preparation process. The formula is prepared from the following components according to the ratios: 2 to 5 parts of Pluronic F68, 5 to 9 parts of PEG6000 dry powder, 1 to 3 parts of CaCl2, 0.3 to 1.5 parts of CH3COONa and the balance of double distilled water. The formula disclosed by the invention is reasonable in design; an antibody can be rapidly and simply extracted aiming at immune eggs; almost lipids and lipoprotein in yolks can be removed; particularly, identification shows that the lipid residue of a purified IgY antibody is less than 8 percent; after the purified IgY antibody is identified by SDS-PAGE, the purity has no difference from that of a common water extraction method and the like.

Description

technical field [0001] The invention belongs to the field of methods for preparing IgY, and in particular relates to a formula of fat-removing liquid in the process of preparing IgY and a preparation method thereof. Background technique [0002] IgY (eggyolkantibody, IgY) antibody is produced in the body by immunizing poultry (chicken) with a specific antigen, which is passed from the mother to the egg yolk through the blood, enriched in the egg yolk, and then separated from the egg yolk to obtain more specificity. Cloned antibodies. Compared with obtaining antibodies from mammalian serum, it avoids routine animal blood collection and reduces the pain of animals. At the same time, antibodies are obtained through egg yolk, with large yield and simple preparation. 50-100mg of antibodies can be purified from each egg, and the hen It is easy to raise, low in cost, convenient for large-scale production of specific antibodies, and has obvious economic advantages. [0003] Simila...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/02C07K1/14
CPCC07K16/02
Inventor 张小莺任昊徐梦飞
Owner NORTHWEST A & F UNIV
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