NT-proBNP fluorescence immunoassay reagent and preparing method thereof

A fluorescent immunology, nt-probnp technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of ELISA reagent instability, poor red blood cell interception effect, serum experiment interference, etc., to achieve reasonable and effective component formula, Effects with high sensitivity and a wide range of applications

Active Publication Date: 2015-11-25
NINGBO RUI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The N-terminal pro-BNP detection reagents of some domestic companies use colloidal gold technology, etc., which have many shortcomings, such as unstable enzyme-linked immunosorbent reagents, serious interference in serum experiments, poor interception effect of sample pads on red blood cells, and poor resolution. Low, colloidal gold is qualitative detection or semi-quantitative detection, etc.

Method used

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  • NT-proBNP fluorescence immunoassay reagent and preparing method thereof
  • NT-proBNP fluorescence immunoassay reagent and preparing method thereof
  • NT-proBNP fluorescence immunoassay reagent and preparing method thereof

Examples

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Embodiment 1

[0040] A kind of NT-proBNP fluorescence immunity reagent, described NT-proBNP fluorescence immunity reagent comprises labeling with NT-proBNP1 antibody, labeling with goat anti-chicken IgY antibody, coating with NT-proBNP2 antibody, coating with chicken IgY antibody, particle weight Suspension, sample pad treatment solution, particle resuspension: containing 250μg / ml mouse IgG, 1% calf serum, 0.1% PluronicF68, 0.6% casein, 0.9% NaCl, 0.05% NaN 3 , Tris-HCl resuspension of 100mM Tris, pH8; wherein, the mouse IgG and calf serum were all treated at 55°C for 10min before adding the particle resuspension; sample pad treatment solution: 10mMPBS (containing 0.1mg / ml lectin), pH7.

Embodiment 2

[0042] A kind of NT-proBNP fluorescence immunity reagent, described NT-proBNP fluorescence immunity reagent comprises labeling with NT-proBNP1 antibody, labeling with goat anti-chicken IgY antibody, coating with NT-proBNP2 antibody, coating with chicken IgY antibody, particle weight Suspension, sample pad treatment solution, particle resuspension: containing 300μg / ml mouse IgG, 5% calf serum, 0.3% PluronicF68, 0.8% casein, 0.9% NaCl, 0.05% NaN 3 , Tris-HCl resuspension of 200mM Tris, pH9; wherein, the mouse IgG and calf serum were all treated at 60°C for 10min before adding the particle resuspension; sample pad treatment solution: 10mMPBS (containing 0.4mg / ml lectin), pH7.

Embodiment 3

[0044] A kind of NT-proBNP fluorescence immunity reagent, described NT-proBNP fluorescence immunity reagent comprises labeling with NT-proBNP1 antibody, labeling with goat anti-chicken IgY antibody, coating with NT-proBNP2 antibody, coating with chicken IgY antibody, particle weight Suspension, sample pad treatment solution, particle resuspension: containing 400μg / ml mouse IgG, 2% calf serum, 0.5% PluronicF68, 0.5% casein, 0.9% NaCl, 0.05% NaN 3 , Tris-HCl resuspension of 150mM Tris, pH8-9; wherein, the mouse IgG and calf serum were all treated at 55°C for 10min before adding the particle resuspension; sample pad treatment solution: 10mMPBS (containing 0.5 mg / ml lectin), pH8.

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Abstract

The invention relates to an NT-proBNP fluorescence immunoassay reagent and a preparing method thereof, which belong to the technical field of medical detection. The NT-proBNP fluorescence immunoassay reagent comprises an NT-proBNIP antibody for marking, a goat anti-chicken IgY antibody for marking, an NT-proBNP2 antibody for coating, a chicken IgY antibody for coating, a particle resuspension and a sample pad conditioning fluid, wherein the particle heavy suspension is Tris-HCl heavy suspension with 100-200mM Tris (containing 250-500mu.g / ml mouse IgG, 1-5% of calf serum, 0.1-0.5% of Pluronic F68, 0.5-1% of casein, 0.9% of NaCl, 0.05% of NaN3) and has a pH being 8-9, the sample pad conditioning fluid contains 10mM PBS (containing 0.1-0.5mg / ml of phytolectin) and has a pH being 7-8. The invention specifically discloses a preparing method of the NT-proBNP fluorescence immunoassay reagent, comprising specific particle marking, sample pad pretreatment, antibody coating on an NC film, and test paper assembling. The NT-proBNP fluorescence immunoassay reagent is high in sensitivity, wide in application range and capable of reducing interference in a serum test, and has a good interception effect on red cells.

Description

technical field [0001] The invention relates to an NT-proBNP fluorescent immunological reagent and a preparation method thereof, belonging to the technical field of medical inspection and determination. [0002] The meanings of the terms involved in this patent document: [0003] 0.9% NaCl: means that every 100ml of the prepared solution contains 0.9g of NaCl. [0004] Similar formats mean similar meanings, unless otherwise stated herein. Background technique [0005] Heart failure is the terminal stage of various cardiovascular diseases and has a high mortality rate, which is equivalent to some malignant tumors. Studies have shown that the mortality rate of heart failure is 43%, and the 5-year mortality rate reaches 90%. rate is higher. Brain natriuretic peptide (BNP) is secreted when the ventricle is stimulated by stretch or tension increases, and it can early reflect the functional changes caused by the overall or even local cardiac structural changes. BNP comes from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533
CPCG01N33/533
Inventor 蒋海张闻周海滨王建飞
Owner NINGBO RUI BIO TECH
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