Heat-resisting glucamylase as well as coding gene and application thereof

A technology of glucoamylase and coding gene, which is applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of Mucor micromycetes that have not been reported, and achieve the effect of good thermal stability
CN103122342AActive Publication Date: 2013-05-29EAST CHINA UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Publication Date
2013-05-29

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Abstract

The invention provides a heat-resisting glucamylase which is protein meeting the following condition (a) or (b): (a) the protein contains an amino acid sequence shown as SEQ ID NO.22; and (b) the protein contains a sequence which is at least 70% homologous with the amino acid sequence shown as the SEQ ID NO.22. The coding gene provided by the invention is deoxyribonucleotide meeting the following conditions (1), (2) and (3): (1) the deoxyribonucleotide contains the amino acid sequence shown as SEQ ID NO.20; (2) the deoxyribonucleotide contains the amino acid sequence shown as SEQ ID NO.21; and (3) the deoxyribonucleotide contains a sequence which is at least 705 homologous with the amino acid sequence shown as the SEQ ID NO.20 and the SEQ ID NO.21. The recombinant host cell provided by the invention comprises a recombinant expression vector containing the coding gene. An optimum temperature of glucamylase provided by the invention is higher than 70 DEG C, thermal stability of the glucamylase is good, and industrial application requirement is met. The recombinant host cell provided by the invention is applicable to expression of the glucamylase gene; expression is induced by shaking a flask, wherein expression quantity of KM71 protein is 0.7g / L; and an industrialization application prospect can be achieved.
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Description

technical field

[0001] The invention relates to a glucoamylase with better thermal stability, and also relates to the encoding gene and application of the thermostable glucoamylase. Background technique

[0002] Glucoamylase (α-1,4-Glucanglucohydrolases, EC3.2.1.3) is an exo-active glycosidase, which can use starch, dextrin and other polysaccharides or oligosaccharide carbon chains as substrates to convert from non-reducing glucoamylases. End-to-end hydrolysis of α-1,4-glucosidic bonds one by one to generate β-D-glucose, and can also slowly hydrolyze α-1,6-glucosidic bonds of amylopectin to generate glucose. It is one of the main enzymes in the process of hydrolyzing starch to generate glucose. First, it has important commercial value in the fields of food processing, ethanol production and brewing.

[0003] The glucoamylase currently used in the market is basically produced by fermentation of Aspergillus niger. Although the enzyme activity is high, the optimum temperature ...

Claims

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