Method and kit for detecting correlated mutation allele of total farrow number of sow as well as application

A technology of total litter size and alleles, applied in the field of alleles, can solve the problems of lack of correspondence, low selection efficiency, and inability to directly know individual genotypes, etc., to achieve economic benefits, high application value, and detection costs cheap effect

Inactive Publication Date: 2010-11-24
BEIJING HEILIU ANIMAL HUSBANDRY TECH
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Problems solved by technology

Pig breeding is mainly divided into conventional breeding and molecular breeding. In traditional breeding, the basis of selection is usually phenotype rather than genotype. This is because people canno

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  • Method and kit for detecting correlated mutation allele of total farrow number of sow as well as application
  • Method and kit for detecting correlated mutation allele of total farrow number of sow as well as application
  • Method and kit for detecting correlated mutation allele of total farrow number of sow as well as application

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Embodiment 1

[0019] Embodiment 1: the selection of excellent breeding pigs in the breeding of pigs

[0020] 1. Establishment of PCR-SSCP detection method and determination of polymorphic sites

[0021] 1. PCR amplification

[0022] Primers were designed according to the porcine GenBank Accession Number AX752829 sequence, and the primers were as follows:

[0023] U (upstream primer): 5'-ATCTCCCGTGGCCATATTCT-3' (SEQ ID NO: 1);

[0024] D (downstream primer): 5'-ATGGCAGGCTGGATGAAG-3' (SEQ ID NO: 2).

[0025] The Beijing black pig was selected as the experimental material. Using the genomic DNA of Beijing black pig as a template, PCR amplification was performed using primers U and D.

[0026] Amplification system: Genomic DNA 200ng, 10×PCR amplification buffer 2.5μl, dNTPs 5mM, upstream and downstream primers 50ng each, Taq DNA polymerase 0.75U, Mg 2＋ 2.5mmol / L, with ddH 2 O Supplement the reaction system to 25 μl.

[0027] The PCR reaction conditions were: denaturation at 95°C for 5 min...

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Abstract

The invention discloses a method and a kit for detecting the correlated mutation allele of a total farrow number of a sow as well as the application. The method adopts a PCR (Polymerase Chain Reaction)-SSCP (Single-Strand Conformation Polymorphism) method, PCR augmentation comprises a section from 315-bit deoxyribonucleotide at the 5' terminal of Gen Bank Accession Number AX752829, PCR augmentation products are detected by gel electrophoresis, and the gene type of the sow to be detected is confirmed according to an electrophoresis result: the gene type of the sow to be detected can be GG or AA which means a homozygote if the electrophoresis result presents two straps, and the gene type of the sow to be detected can be GA which means a heterozygote if the electrophoresis result presents three straps. The detection method of the invention has simple operation, low expense and high accuracy, can realize the automatic direct detection and has higher practical breeding application values. The method and the kit of the invention can be applied to breed grices, early select grices to be selected, effectively solve the problem of long time for selecting excellent grices in practical production, decrease the breeding expense, increase the total sow farrow number and improve the economic benefits.

Description

technical field [0001] The invention relates to a method for detecting mutant alleles related to total litter size of sows, a kit and application thereof in breeding pigs with high total litter size traits and pig breeding. Background technique [0002] Fecundity is an important economic trait that affects the pig industry. The improvement of fecundity will undoubtedly bring huge economic benefits to pig production, but how to improve the breeding efficiency of pig fecundity has always been a concern. Pig breeding is mainly divided into conventional breeding and molecular breeding. In traditional breeding, the basis of selection is usually phenotype rather than genotype. This is because people cannot directly know the genotype of an individual and can only infer it from the phenotype. . The lack of a clear correspondence between phenotypes and genotypes for quantitative traits makes selection inefficient. To improve the efficiency of selection, the most ideal method should...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 谢蜀杨王红卫石国华张龙超王立贤
Owner BEIJING HEILIU ANIMAL HUSBANDRY TECH
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