Method for detecting sow total-number-born character-correlated mutant alleles and reagent kit thereof

A technology of total litter size and detection method, applied in the field of in situ genes, can solve the problems of low selection efficiency, lack of corresponding relationship, and inability to directly know individual genotypes, etc., achieve high application value, improve economic benefits, and low detection costs Effect

Inactive Publication Date: 2011-01-12
TIANJIN INST OF ANIMAL HUSBANDRY & VETERINARY
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Problems solved by technology

Pig breeding is mainly divided into conventional breeding and molecular breeding. In traditional breeding, the basis of selection is usually phenotype rather than genotype. This is because people cannot directly know the genotype of an individual and can only infer it from the phenotype.
Inefficient selection due to lack of clear correspondence between phenotypes and genotypes for quantitative traits

Method used

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  • Method for detecting sow total-number-born character-correlated mutant alleles and reagent kit thereof
  • Method for detecting sow total-number-born character-correlated mutant alleles and reagent kit thereof
  • Method for detecting sow total-number-born character-correlated mutant alleles and reagent kit thereof

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Experimental program
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Embodiment 1

[0029] Embodiment 1, establishment of breeding method

[0030] 1. Establishment of PCR-SSCP detection method and determination of polymorphic sites

[0031] 1. PCR amplification

[0032] Primers were designed according to the pig GenBank Accession Number EU407778 sequence, and the primers were as follows:

[0033] U (upstream primer): 5'-ACTACCCCCTTGTCCCAAAC-3' (sequence 1 of the sequence listing);

[0034] D (downstream primer): 5'-GGACCAA GCCAATCAGAGAA-3' (SEQ ID NO: 2 of the Sequence Listing).

[0035] Large white pigs were selected as experimental materials. Using large white pig genomic DNA as a template, PCR amplification was performed using primers U and D.

[0036] Amplification system: 10×buffer 2.0μL, 25μmol / L Mg2+2.0μL, 10μmol / L dNTPs 0.4μL, 10μmol / L upstream and downstream primers 0.4μL, template DNA 50ng, Taq DNA polymerase 0.3μL, ultra-pure water to 20 μL.

[0037] The PCR reaction conditions were: denaturation at 95°C for 5 min; then denaturation at 95°C f...

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Abstract

The invention discloses a method for detecting sow total-number-born character-correlated mutant alleles and a reagent kit thereof. The method provided by the invention comprises the following step of: determining whether the genotype of a sow to be detected is TT or CT by detecting whether the 2348th-bit deoxyribonucleotide of the sow GenBank Accession Number EU407778 to be detected is T or C, wherein the number born of the sow in the TT genotype is higher than that of the sow in the CC genotype, the TT genotype is the homozygote of the GenBank Accession Number EU407778 with the 2348th-bit deoxyribonucleotide as T from a 5' terminal, and the CC genotype is the homozygote of the GenBank Accession Number EU407778 with the 2348th-bit deoxyribonucleotide as C from the 5' terminal. Pigs are bred by applying the method provided by the invention, and the early screening for the pigs to be selected can be carried out, thereby effectively relieving the problem of long time for selecting excellent boars in actual production, lowering the breeding expenses, effectively increasing the total number born of the pigs in the actual production, improving the economic benefit, realizing the automated detection and exerting a great action in pig breeding.

Description

technical field [0001] The invention relates to a method for detecting mutant alleles related to the total litter size of sows and a special kit thereof. Background technique [0002] Fecundity is an important economic trait that affects the pig industry. The improvement of fecundity will undoubtedly bring huge economic benefits to pig production, but how to improve the breeding efficiency of pig fecundity has always been a concern. Pig breeding is mainly divided into conventional breeding and molecular breeding. In traditional breeding, the basis of selection is usually phenotype rather than genotype. This is because people cannot directly know the genotype of an individual and can only infer it from the phenotype. . The lack of a clear correspondence between phenotypes and genotypes for quantitative traits makes selection inefficient. To improve the efficiency of selection, the most ideal method should be to be able to directly select the genotype. The genotype of molec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 李千军王立刚穆淑琴马墉赵宏志高荣玲张春华
Owner TIANJIN INST OF ANIMAL HUSBANDRY & VETERINARY
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