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A method for identifying pig backfat thickness and its special primer pair

A technology of backfat thickness and primer pairs, applied in biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve problems such as slow genetic progress, increased breeding costs, and prolonged generation intervals. Achieve high application value, reduce breeding costs, and low testing costs

Inactive Publication Date: 2017-01-18
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since indicators such as backfat thickness can only be measured after the animal grows up, on the one hand, it increases the cost of breeding, on the other hand, it prolongs the generation interval, and the genetic progress is slow.

Method used

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  • A method for identifying pig backfat thickness and its special primer pair
  • A method for identifying pig backfat thickness and its special primer pair
  • A method for identifying pig backfat thickness and its special primer pair

Examples

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Effect test

Embodiment 1

[0030] Embodiment 1, identification of pig backfat thickness size

[0031] 1. Determination of g.13739C>T polymorphism site of porcine NUDT3 gene

[0032] (1) Two Damin hybrid pigs were used as experimental materials, and the genomic DNA of the ear margin tissues were extracted respectively.

[0033] (2) Design and synthesis of primers

[0034] According to the genomic DNA sequence of porcine NUDT3 gene (GenBank Accession Number NC_010449.4), the following primers were designed and synthesized:

[0035] U (upstream primer): 5'-ATCACTTCCACTCCACTCA-3' (SEQ ID No.1);

[0036] D (downstream primer): 5'-GCTCTCGGTCTCAGTTCT-3' (SEQ ID No. 2).

[0037] (3) PCR amplification

[0038] The genomic DNA of Damin hybrid pig obtained in step (1) was used as a template, and U and D were used as primers to carry out PCR amplification to obtain PCR amplification products, which were named product 1 and product 2, respectively.

[0039] PCR amplification system: Genomic DNA 200ng, 10×PCR am...

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Abstract

The invention discloses a method for identifying the pig back fat thickness and a special primer pair thereof. The invention discloses a primer pair for amplifying a DNA fragment with g.13739C>T polymorphic site, wherein the g.13739C>T polymorphic site is the 13739th site from the 5' end of a sequence with GenBankAccession Number NC_010449.4; and the GenBankAccession Number is the update time (September 29, 2013) of the sequence NC_010449.4. By adopting the method disclosed by the invention to breed pigs, the candidate pigs can be screened early, so that the breeding cost is reduced, and the back fat thickness of the pigs in actual production can be effectively reduced or increased. The method disclosed by the invention is simple to operate, requires low cost, has high accuracy, can realize automatic direct detection, and plays a great role in pig breeding.

Description

technical field [0001] The invention relates to a method for identifying pig backfat thickness and a pair of special primers, belonging to the field of biotechnology. Background technique [0002] The backfat thickness of pigs has always been an important part of pig breeding goals. The thickness of backfat is not only related to lean meat percentage, but also has a great impact on growth rate, so its breeding value is very high. However, since indicators such as backfat thickness can only be measured after the animal grows up, on the one hand, the cost of breeding is increased, and on the other hand, the generation interval is lengthened, and the genetic progress is slow. The rapid development of molecular biotechnology and the construction of pig genetic linkage maps provide a research basis for more accurate and faster molecular breeding of quantitative traits such as backfat thickness. On the other hand, since pigs are an important model animal for human research, findi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 王立贤王立刚张龙超颜华刘欣
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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