Aptamer capable of being specifically combined with nodularin-R and application of the aptamer

Abstract

The invention relates to the technical field of bio-medicine engineering, and provides an aptamer capable of being specifically combined with nodularin-R and an application of the aptamer. A sequencegeneral formula of the aptamer is 5'-AAGGAGCAGCGTGGAGGATA-N40-TTAGGGTGTGTCGTCGTGGT-3', wherein N is any one of the four deoxyribonucleotide basic groups A, T, G and C, the number 40 refers to the number of random basic groups, preferably a sequence represented as anyone from the SEQ ID No.1 to the SEQ ID No.7, most preferably, anyone from the SEQ ID No.9 to SEQ ID No.11. The aptamer can be used for preparing an aptamer sensor or a detection reagent applied to detection of the NOD-R for a drinking water sample. The products also can be a basis for preparing medicines for preventing or treatingNOD-R poisoning and removing the NOD-R from water body or aquatic products.

Description

technical field

[0001] The invention relates to the technical field of biomedical engineering, in particular to an aptamer specifically binding to nodularin-R and its application. Background technique

[0002] Nodularin-R (Nodularin-R, NOD-R) is a strong cyanobacterial hepatotoxin, mainly produced by the filamentous planktonic cyanobacteria Nodularia spumigena. Studies have shown that NOD-R not only has a cancer-promoting effect, but also is a carcinogen, and its mechanism of action is to inhibit the activity of serine / threonine protein phosphatase 1 (PP1) and 2A (PP2A), thereby exerting toxic effects. Since 1988, researchers have discovered ten kinds of NOD, among which NOD-R is the most abundant and the only commercialized toxin. NOD-R is easily soluble in water, and has strong heat resistance, and the toxin cannot be destroyed by heating and boiling. People are mainly poisoned by drinking contaminated water and eating contaminated aquatic products. Symptoms of poisoning...

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