97results about How to "Short purification cycle" patented technology

The invention provides a purification technology for low-grade bentonite, belonging to the field of processing and utilization of non-metal ores. The purification technology comprises the following steps of preprocessing the raw materials; stirring to prepare pulp; ultrasonically purifying; standing for precipitating; separating out upper-layer slurry; removing impurities through centrifuging; filtering and drying. The purification technology is supported by ultrasonic waves; in the presence of a dispersing agent, the impurities can be well separated from montmorillonite, so that the purification effect is improved; furthermore, the purification technology is simple, is small in equipment investment, can be used for purifying the product with high content of montmorillonite from the low-grade bentonite rapidly, efficiently, simply and conveniently, has the advantages of low production cost and short purification period, and is easy to popularize.

The invention provides a method for purifying a glycopeptide compound, which comprises the following steps: A) loading a sample of aqueous solution of a glycopeptide compound onto an inverse polymer filler Uni PS; B) pre-washing by using pre-washing solution; and C) eluting with eluting solution. When the method provided by the invention is used, the quality of target components of the glycopeptide compound can be improved effectively, the purification period is shortened, and the yield loss is reduced. In addition, the method can be used for preparing a small amount of sample of the glycopeptide compound in a laboratory, and also can be used for industrial production of the glycopeptide compound.

The invention provides a separation and purification method for dalbavancin. The method comprises the following steps: dissolving and filtering a crude dalbavancin extract; loading a sample of a crude solution obtained after filtering into a chromatographic column equipped with polystyrene high-molecular polymer microspheres for chromatography; carrying out gradient elution on a target product with an aqueous phosphoric acid solution and an organic solvent as mobile phases; and collecting solutions with a target peak value in sections and combining constituent solutions meeting requirements. The separation and purification method is simple, can realize large-scale production and has a high and stable recovery rate.

The invention belongs to the technical field of animal cell culture, and specifically relates to a neuron primary culture purification method which comprise the following steps: providing nerve tissues, performing enzymatic hydrolysis and digesting tissues, separating nerve cells with a density gradient centrifugation method, inoculating nerve cells, separating nerve cells by using the differential velocity adhesion characteristic, purifying nerve cell with cytosine arabinoside (Ara-C) to obtain neurons. The neuron primary culture purification method largely shortens primary culture purifying period of DRG neurons, largely promotes the purification of the neurons, guarantees cell activities, facilitates cell culture and growth, and lays a good foundation on the further experiments.

The invention provides a purification device for high-purity indium. The purification device comprises a quartz tube, a plurality of resistance heaters and a guide rail. Two ends of the quartz tube are fixed along the horizontal directions by the aid of a left flange sleeve and a right flange sleeve, and a graphite boat is arranged in the quartz tube; the resistance heaters are arranged on the outer side of the quartz tube and are sequentially arranged along the horizontal directions of the quartz tube, and cooling devices are arranged between the adjacent resistance heaters; the resistance heaters are fixed by the aid of the guide rail, and the guide rail is provided with a left stopper, a slide wire groove and a right stopper. Compared with the prior art, the purification device for thehigh-purity indium has the advantages that the resistance heaters are sequentially arranged on the outer side of the quartz tube along the horizontal directions, and accordingly zone melting and directional solidification can be simultaneously implemented; the temperatures of the resistance heaters are controlled, multistage heating can be implemented, uniform heating temperature gradient distribution and stable temperature fields can be guaranteed, accordingly, integral purification procedures are high in efficiency, and 6N-7N ultrahigh-purity indium production requirements can be met.

The invention discloses an efficient secretory expression and purification method of a recombinant urate oxygen oxidoreductase, and belongs to the field of medical and biological engineering. The technical scheme comprises the following steps of: expressing an escherichia coli operon of the recombinant urate oxygen oxidoreductase using the encoding sequence of a recombinant urate oxygen oxidoreductase which is artificially synthesized from the encoding sequence of an escherichia coli alkaline phosphatase promoter and the encoding sequence of an escherichia coli LamB signal peptide; cloning the operon to a plasmid vector pBR322 carrying the escherichia coli to obtain an expression plasmid; transforming a W3110 escherichia coli empty host using the expression plasmid containing the escherichia coli operon to obtain an engineering strain for the secretory expression of the recombinant urate oxygen oxidoreductase; and carrying out purification technologies of cation-exchange chromatography, hydrophobic chromatography, and anion-exchange chromatography on the recombinant urate oxygen oxidoreductase. The method disclosed by the invention has the beneficial effects that a good secretory expression effect can be achieved through the regulation and control on protein expression by the escherichia coli alkaline phosphatase promoter and induction of the secretory expression of the urate oxygen oxidoreductase by the optimized escherichia coli LamB signal peptide, and the purification method is simple and easy in implementation, short in protein purification period and high in protein yield.

The invention relates to a method for preparing and purifying an olmesartan intermediate. The preparation method comprises the following steps of: chlorinating 4,5-dimethyl-1,3-dioxa-cyclopentene-2-ketone, distilling under reduced pressure to obtain 4-chloro-4-methyl-5-methylene-1,3-dioxolane-2-ketone, and performing rearrangement reaction to generate the olmesartan intermediate; reducing the temperature to be below 50 DEG C, concentrating until a solvent is removed completely to obtain a crude product; and recrystallizing and purifying the crude product at the temperature of between -20 and 0 DEG C for 1 to 48 hours, wherein a recrystallization solvent may be one or a mixed solvent of more of an alkane solvent and an ether solvent. The preparation method is low in production cost, mild in reaction condition and easy to operate, raw materials are wide in sources, and high-content 4-chloromethyl-5-methyl-1,3-dioxa-cyclopentene-2-ketone can be obtained directly, so the method is particularly suitable for large-scale industrial production.

The invention relates to a filler special for purifying semaglutide and a purification method of semaglutide. The method specifically comprises the steps of dissolving crude semaglutide into an aqueous solution of acetonitrile to obtain a crude peptide solution; conducting filtering, mixing a reverse phase and an ion exchange filler into a fixed phase in a certain proportion, after a filtrate is loaded, conducting HPLC linear gradient elution simultaneously with a moving phase mixed by an organic phase and a salt phase, and collecting distillate containing semaglutide; taking the third distillate, and conducting reduced-pressure rotary evaporation concentration and freeze drying to obtain the filler. Semaglutide with the purity of 30% or so can be purified to 90% or above in one step; meanwhile, the loading quantity is high, industrial scale production is simple, the purification period is short, and the production cost of enterprises is greatly reduced.

The invention discloses a method for purifying plasma functional proteins from a Cohn fraction IV. The method comprises the following steps: carrying out pretreatment and removing a filter aid and other proteins in the raw materials; carrying out gradient separation by chromatography and collecting the plasma functional proteins in sequence; carrying out sterilization and freeze-drying. The method is high in protein yield and low in cost, comprises the purification process with the pipeline characteristic, has high degree of automation, is suitable for industrialization and achieves comprehensive utilization of the byproduct in plasma product production.

The invention discloses a micro-sample preparation device for a frozen electron microscope and a sample preparation method. The micro-sample preparation device comprises a supporting net assembly witha sample preparation operation state and a freezing operation state, a sample preparation probe used for respectively carrying out sampling operation and completing sample preparation operation on ato-be-detected sample solution on the supporting net assembly, a three-dimensional translation platform used for driving the sample preparation probe to be converted between the sampling operation andthe sample preparation operation, a freezing module used for carrying out freezing operation on the sample solution on the supporting net assembly, and a rapid transfer device used for driving the supporting net assembly to transfer between the sample preparation operation state and the freezing operation state. According to the invention, a frozen electron microscope sample preparation experiment with ultramicro sample consumption can be realized, and the consumption of a single sample liquid drop is in a nano-liter level and below the nano-liter level. Compared with a conventional sample preparation robot, the sample consumption is reduced by 1000 times or above, the experiment cost is remarkably reduced, and the protein sample purification period is shortened. The success rate of frozen electron microscope sample preparation and the reproducibility of experiments are obviously improved.

The invention discloses a method for separation and purification of a nanobody, and relates to the technical field of biology. The method comprises the following steps: (1) precipitating impure protein at low pH: regulating pH of a to-be-purified sample to acidity with an acid solution, after standing, performing centrifugation to remove precipitates, and keeping a supernatant solution; (2) precipitating the impure protein at high temperature: heating the supernatant solution obtained in step (1), after heat-preservation standing, performing centrifugation to remove precipitates, and keeping asupernatant solution; and (3) removing the impure protein with an ultrafiltration method: performing ultrafiltration on the supernatant solution obtained in step (2), wherein a protein solution permeating through an ultrafiltration membrane is the high-purity nanobody. The method provided by the invention does not affect the activity of the nanobody, and has simple operation, low cost and a wideapplication range.

The invention relates to a separation and purification method of gold in gold platinum alloy waste. The method comprises steps as follows: 1), gold platinum alloy waste is weighed, aqua regia is heated and dissolved, concentrated HCl is added while concentrated to remove nitric acid, and water is added to obtain a dilution solution; 2), the dilution solution obtained in the step 1) is heated and adjusted to be alkaline, the solution is stirred, and H202 is added until precipitation occurs in the solution; 3), the step 2) is repeated, and excessive H202 is added continuously; 4), the solution obtained in the step 3) is heated to remove H2O2, filtration is performed, and obtained precipitation is cleaned with distilled water; 5), water is added to the precipitation obtained in the step 4) and boiled, filtration is performed, and precipitation is washed with water to remove CI<-1>; and 6), the precipitation obtained in the step 5) is roasted, and solid gold is obtained. With the adoption of the method, the recovery rate of gold is up to 99% or above, the purity of gold is up to 99.9%, no impurity is brought in a gold purification and separation process, a subsequent platinum purification processing step is reduced, the platinum purification period is shortened, and the platinum purification cost is reduced.

The invention relates to a purification method of a polyethylene glycol recombinant human granulocyte colony-stimulating factor. Particularly, the method for performing cation exchange chromatographic separation and purification of the PEG-G-CSF and preparing PEG-G-CSF stock solution by proper buffer solution substitution is optimized, and the PEG-G-CSF with HPLC (high performance liquid chromatography) and electrophoresis purity of 98% or more can be obtained by one-step column chromatographic purification and buffer solution substitution. By the purification process, process routes are simplified, production time is saved, protein recovery rate is high, the obtained protein stock solution is high in purity, proper medical auxiliary materials are added, so that a medicinal preparation can be prepared, and the purification method is quite suitable for large-scale industrial enlarged production.

The invention provides a method for efficiently separating and purifying porcine ganglioside, which comprises the steps of: dissolving and filtering a crude extract of the pig ganglioside; applying afiltered solution to a chromatography column filled with a homogenous normal-phase silica gel for chromatography; using ultrapure water and an organic solvent as a flow phase for elution of a target product; collecting the solution of the target peak in stages, and collecting the component liquid meeting the requirements. The method is applied to the deep purifying of the pig ganglioside, and onlyneeds one-step chromatography to satisfy the requirement that the pig ganglioside purity is greater than 99% and the yield is greater than 93.0%. The purification yield is high and stable, and the separation method of the invention is simple and convenient, can be used for large-scale production, and the production cost is greatly reduced.

The invention provides a purification method of a recombinant human granulocyte colony-stimulating factor. The method specifically comprises the steps of selecting a cation chromatographic column to purify renaturation solution of the recombinant human granulocyte colony-stimulating factor (rh-G-CSF), and then performing proper buffer solution replacement to prepare high purity rh-G-CSF stock solution, wherein fillers in the cation chromatographic column is in a cationic type with a mixed mode. According to the purification technology provided by the invention, a technical path is simplified, production time is saved, protein recovery rate is high, the acquired protein stock solution is high in purity, a medicine preparation can be produced by adding proper pharmaceutical adjuvant, and the purification technology is very suitable for large-scale industrial amplification production. Meanwhile, the invention also provides a method for preparing pegylated recombinant human granulocyte colony-stimulating factor.

The invention provides a method for fast separating and purifying aspongopus protein components resistant to tumors and belongs to the technical field of biology. Through high-temperature treatment, ammonium sulfate chromatography protein and ultrafiltration, fast separation and purification of the aspongopus protein components resistant to tumors are achieved in order to solve the problems that existing methods are cumbersome in procedure and high in requirement for instruments, the price of related chromatographic columns and fillers is high since high performance liquid chromatographs, nucleic acid protein chromatographs and the like are often required for separation and purification, and the purification period is pretty long.

The invention discloses a method for separating and recovering chloroperoxidase (Chloroperoxidase, EC 1.11.1.10, CPO), which utilizes co-precipitation and redissolution method, that is, hydrophilic polymer polyethylene glycol is precipitated and entrained at high salt concentration CPO molecules, so as to enrich the CPO protein concentration, and then use the two-phase extraction technology to efficiently separate and recover high-purity CPO, that is, to separate different proteins by using the different distribution coefficients of the separation target product and impurities in the hydrophilic polymer phase solution. The present invention proposes reasonable and feasible two-phase CPO extraction and purification conditions through research, summarizes the separation technology of micro-content bioactive substances, solves or improves the extraction interference, low recovery rate, high product price, and purification cycle existing in the current CPO production process. Long, can not scale production and other issues.

The invention discloses a method for efficiently adsorbing arsenic in underground water by using nano iron oxide. The method comprises the following steps of preparation of a mesoporous structure, preparation of the nano iron oxide with a particle diameter of less than 10 nanometers, formation of a resin-based nano iron oxide composite material, precipitation treatment, shaking treatment, adsorption wall adsorption purification, mixed centrifugal treatment and cleaning of the resin-based nano iron oxide composite material. The method for efficiently adsorbing the arsenic in the underground water by using the nano iron oxide has the advantages that the mesoporous structure prepared by adopting a rapid freezing method and the nano iron oxide prepared from powdery ferric trichloride can ensure the effect that the particle diameter of the nano iron oxide is less than 10 nanometers, the resin-based nano iron oxide composite material with the nano iron oxide with small particle diameter hashigh adsorption capacity for arsenic, thereby being capable of improving the purification function for the arsenic in the underground water as an adsorption wall, so that while the drinking safety isensured, economic popularization can also be achieved, and the method is suitable for water improvement in areas with quality-induced water shortage in northwest China, and has the advantages of highefficiency and low cost.

The invention relates to the technical field of biomedical engineering, and provides an oncolytic vaccinia virus carrying a tachypleus tridentatus lectin gene, and a construction method and an application thereof. The oncolytic vaccinia virus carries the tachypleus tridentatus lectin gene, and a DNA sequence of the tachypleus tridentatus lectin gene is shown as SEQ ID NO: 1; the construction method of the oncolytic vaccinia virus comprises the following two steps: (A) inserting gene sequences of tachypleus tridentatus lectin into pCB plasmids through Xba I and Bgl II loci to obtain pCB-TTL plasmids; and (B) recombining the pCB-TTL plasmid and the vaccinia virus in cells, after screening and identifying, obtaining the oncolytic vaccinia virus carrying the tachypleus tridentatus lectin gene.The oncolytic vaccinia virus disclosed by the invention not only has a remarkable inhibition effect on various tumor cells, but also can remarkably inhibit the antiviral factor level of the tumor cells and remarkably improve the ERK phosphorylation level, and is beneficial to virus replication; and in addition, the replication level of the oncolytic vaccinia virus carrying the TTL gene is also remarkably improved.