The invention is applicable to the technical field of
crude oil treatment, and provides a method for separating and screening
crude oil degrading functional
bacteria. Comprising the following steps: S1, collecting an environmental sample polluted by
crude oil for a long time; s2, inoculating the environmental sample into a gradient
carbon source inorganic salt
enrichment culture medium for
enrichment culture to obtain an
enrichment culture solution; s3, performing gradient
dilution on the enrichment culture solution,
coating the enrichment culture solution on a selective
solid culture medium containing crude oil, and culturing to obtain a single colony; s4, picking single colonies, inoculating the single colonies into a
liquid culture medium containing crude oil, and determining the crude oil degradation rate of the single colonies; s5, screening out strains with the crude oil degradation rate higher than a preset threshold value, namely the crude oil degradation functional
bacteria. According to the method, the crude oil is used as a unique
carbon source, infectious microbes which can only utilize an easily degradable
carbon source are eliminated from the source, rare strains which really have a crude oil-
enzyme system are enriched, the abundance of target
bacteria can be improved by 2-3 orders of magnitude through first passage, and the subsequent purification period is shortened by more than 50%.