6results about How to "Short purification cycle" patented technology

The present invention relates to a method for purifying crude orlistat, and the process steps include water washing, ceramic membrane filtration, column chromatography, reduced pressure concentration, filtration and drying. The present invention adopts a new adsorbent to replace conventional materials, and only a single organic solvent is used in the extraction process. Compared with the current domestic conventional process, it has the characteristics of short chromatography cycle, small amount of adsorbent, high extraction yield, stable product quality, low production cost, etc., which is conducive to enhancing the foreign market competitiveness of the product.

The invention relates to the technical field of medicine purification, and discloses a method for separating and purifying uridine-5 '-diphosphate-N-acetyl-glucosamine, which comprises the following steps: S1, loading a sample: filtering a uridine-5'-diphosphate-N-acetyl-glucosamine crude product, and loading the filtered uridine-5 '-diphosphate-N-acetyl-glucosamine crude product into a chromatographic column; s2, elution is carried out, wherein the uridine-5 '-diphosphate-N-acetyl-glucosamine adsorbed in the chromatographic column is eluted with an eluent; s3, after collecting, gathering and eluting, carrying out segmented collection, and gathering the component liquid meeting the target peak value requirement; the separation and purification method of uridine-5 '-diphosphate-N-acetyl-glucosamine is simple and convenient, the purification period is short, only a salt solution is used as a mobile phase in the whole purification process, no organic solvent is needed, the method is safe, pollution-free and very low in cost, the used stationary phase can be reused, and the method is suitable for industrial production. The method can be used for large-scale production, the environmental protection pressure and the production cost are greatly reduced, and a better use prospect is brought.

The invention is applicable to the technical field of crude oil treatment, and provides a method for separating and screening crude oil degrading functional bacteria. Comprising the following steps: S1, collecting an environmental sample polluted by crude oil for a long time; s2, inoculating the environmental sample into a gradient carbon source inorganic salt enrichment culture medium for enrichment culture to obtain an enrichment culture solution; s3, performing gradient dilution on the enrichment culture solution, coating the enrichment culture solution on a selective solid culture medium containing crude oil, and culturing to obtain a single colony; s4, picking single colonies, inoculating the single colonies into a liquid culture medium containing crude oil, and determining the crude oil degradation rate of the single colonies; s5, screening out strains with the crude oil degradation rate higher than a preset threshold value, namely the crude oil degradation functional bacteria. According to the method, the crude oil is used as a unique carbon source, infectious microbes which can only utilize an easily degradable carbon source are eliminated from the source, rare strains which really have a crude oil-enzyme system are enriched, the abundance of target bacteria can be improved by 2-3 orders of magnitude through first passage, and the subsequent purification period is shortened by more than 50%.

The invention relates to the technical field of medicine purification, and discloses a method for separating and purifying uridine diphosphate glucuronic acid, which comprises the following steps: S1, loading a sample: filtering a uridine diphosphate glucuronic acid crude product, and loading the filtered uridine diphosphate glucuronic acid crude product into a chromatographic column, the filler of the chromatographic column being a monodisperse microsphere of which the matrix is polyacrylate or polystyrene-divinyl benzene; s2, elution: eluting the uridine diphosphate glucuronic acid adsorbed in the chromatographic column by using an eluent; and S3, collection and gathering: carrying out segmented collection after elution, and gathering the component liquid meeting the target peak value requirement. The separation and purification method of uridine diphosphate glucuronic acid is simple and convenient, has a short purification period, only uses a salt solution as a mobile phase in the whole purification process, does not need to use any organic solvent, and is suitable for large-scale production. The method is safe, pollution-free and very low in cost, the used stationary phase can be reused, the method can be used for large-scale production, the environmental protection pressure and the production cost are greatly reduced, and a better use prospect is brought.

The present application relates to hydrogen purification technical field, a kind of high-efficiency stable palladium tube hydrogen purifier, comprising: shell assembly, shell assembly is made of shell, heat preservation layer and inner container, and heat preservation layer is filled in the gap between shell and inner container, the middle of one end of inner container is fixed with the original gas inlet pipe extending out of shell, and the one end of original gas inlet pipe is fixedly installed with inlet valve;Feed pretreatment component, feed pretreatment component is set in the one end of inner container close to original gas inlet pipe, and its top extends to the top side of shell;End cap, end cap is fixedly installed in the one end of shell away from original gas inlet pipe, and the middle of end cap is fixed with hydrogen outlet pipe.The present application realizes the multistage depth purification of hydrogen, greatly improves the recovery rate and purity of hydrogen after purification, simultaneously, high-efficiency breaks the laminar boundary layer formed by raw material gas on the surface of palladium tube body, reduces the resistance of hydrogen molecules from raw material gas main body to spread to the surface of palladium tube, and realizes the double energy saving of tail gas waste heat recovery and tail gas kinetic energy utilization.

The invention relates to the technical field of medicine purification, and discloses a uridine diphosphate glucose separation and purification method, which comprises the following steps: S1, loading: filtering a uridine diphosphate glucose crude product, loading the filtered uridine diphosphate glucose crude product into a chromatographic column, and taking polystyrene-divinyl benzene monodisperse microspheres as a matrix as a filler of the chromatographic column; s2, elution: eluting the uridine diphosphate glucose adsorbed in the chromatographic column by using an eluent; and S3, collecting and gathering: carrying out segmented collection on the eluted uridine diphosphate glucose solution, and gathering the component liquid meeting the target peak value requirement. The separation and purification method of uridine diphosphate glucose is simple and convenient, the purification period is short, the requirement for high purity of uridine diphosphate glucose can be met only through one-step chromatographic purification, the purity reaches 99% or above, the purification yield is high and stable, and better use prospects are brought.