Neuron primary culture purification method

A neuron culture solution and primary culture technology, applied in the field of neuron culture purification, can solve the problems of unguaranteed purity, indefinite purification times, large cell differences, etc. good vitality effect

Inactive Publication Date: 2016-01-27
NANTONG UNIVERSITY
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Problems solved by technology

At present, in the primary culture process of DRG neurons, cytarabine (Ara-C) is often used to inhibit the growth of glial cells to purify DRG neurons. It takes a long time to obtain high-purity DRG neurons. At the same time, Ara-C also has a certain impact o

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[0033] The specific embodiments of the present invention will be described below in conjunction with the drawings.

[0034] In order to further describe in detail the conventional techniques used in the practice of the present invention, practitioners can refer to relevant standard textbooks and reviews of cell biology, histology, and embryology. Including neurons: Cell and Molecular Biology, United States, L.B. Levitan; L.K. Katzmark.

[0035] Cell culture methods are usually described in the latest edition of "Animal Cell Culture: Basic Technique Manual" (Edited by R.I. Freshney, Wiley & Sons); "General Techniques of Cell Culture" (M.A. Harrison and I.F. Rae, Cambridge University Press). Tissue culture media supplies and reagents can be obtained from commercial suppliers.

[0036] The "mammals" referred to in this article is the highest group of vertebrates, evolved from reptiles. The main features are: the body surface has hair, generally divided into five parts: head, neck, trun...

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Abstract

The invention belongs to the technical field of animal cell culture, and specifically relates to a neuron primary culture purification method which comprise the following steps: providing nerve tissues, performing enzymatic hydrolysis and digesting tissues, separating nerve cells with a density gradient centrifugation method, inoculating nerve cells, separating nerve cells by using the differential velocity adhesion characteristic, purifying nerve cell with cytosine arabinoside (Ara-C) to obtain neurons. The neuron primary culture purification method largely shortens primary culture purifying period of DRG neurons, largely promotes the purification of the neurons, guarantees cell activities, facilitates cell culture and growth, and lays a good foundation on the further experiments.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to cell culture, in particular to a purification method for neuron culture. Background technique [0002] Nerve cells (including neurons and glial cells) are the basic units that constitute the structure and function of the nervous system. Neurons are cells with long processes, which consist of a cell body and cell processes. Neurons are cells with long synapses (axons), which consist of a cell body and cell processes. The soma of neurons is located in the gray matter and ganglia of the brain and spinal cord, and has various shapes, such as star, cone, pear, and sphere. Cell bodies vary in size, with a diameter between 5 and 150 μm. The cell body is the metabolic and nutritional center of the neuron. [0003] Dorsal root ganglion neuron (dorsal root ganglion, DRG) is the first relay station that transmits primary sensory information to the central nervous system, and plays an import...

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Application Information

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IPC IPC(8): C12N5/0793
Inventor 施海燕丁斐强亮李晓丽张石波郑礼钧高佳文孙晓婷
Owner NANTONG UNIVERSITY
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