Neuron primary culture purification method

Abstract

The invention belongs to the technical field of animal cell culture, and specifically relates to a neuron primary culture purification method which comprise the following steps: providing nerve tissues, performing enzymatic hydrolysis and digesting tissues, separating nerve cells with a density gradient centrifugation method, inoculating nerve cells, separating nerve cells by using the differential velocity adhesion characteristic, purifying nerve cell with cytosine arabinoside (Ara-C) to obtain neurons. The neuron primary culture purification method largely shortens primary culture purifying period of DRG neurons, largely promotes the purification of the neurons, guarantees cell activities, facilitates cell culture and growth, and lays a good foundation on the further experiments.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to cell culture, in particular to a purification method for neuron culture. Background technique [0002] Nerve cells (including neurons and glial cells) are the basic units that constitute the structure and function of the nervous system. Neurons are cells with long processes, which consist of a cell body and cell processes. Neurons are cells with long synapses (axons), which consist of a cell body and cell processes. The soma of neurons is located in the gray matter and ganglia of the brain and spinal cord, and has various shapes, such as star, cone, pear, and sphere. Cell bodies vary in size, with a diameter between 5 and 150 μm. The cell body is the metabolic and nutritional center of the neuron. [0003] Dorsal root ganglion neuron (dorsal root ganglion, DRG) is the first relay station that transmits primary sensory information to the central nervous system, and plays an import...

AI Technical Summary

Problems solved by technology

At present, in the primary culture process of DRG neurons, cytarabine (Ara-C) is often used to inhibit the growth of glial cells to purify DRG neurons. It takes a long time to obtain high-purity DRG neurons. At the same time, Ara-C also has a certain impact on the cell viability of neurons. In addition, different manufacturers and batch numbers of Ara-C reagents will result in variable purification times. , the purity cannot be guaranteed, and the differences of cells cultured in different batches are large, etc.

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