Method for fast separating and purifying aspongopus protein components resistant to tumors

A technology of separation and purification, Jiuxiangworm, which is applied in the biological field, can solve the problems of expensive chromatographic column packing, long purification cycle, and high instrument requirements, and achieve the effect of short purification cycle, low cost, and low instrument requirements

Active Publication Date: 2018-06-12
GUIZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The object of the present invention is to provide a method for rapidly separating and purifying the anti-tumor protein components of Nine-scented Insect, so as to solve the problem that the existing methods have complicated steps and high requirements for instruments, and often require high-performance liquid chromatography, nucleic acid protein chromatography, etc. For separation and purification, the chromatographic columns and fillers involved are relatively expensive, and the purification cycle is long, etc.

Method used

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  • Method for fast separating and purifying aspongopus protein components resistant to tumors
  • Method for fast separating and purifying aspongopus protein components resistant to tumors
  • Method for fast separating and purifying aspongopus protein components resistant to tumors

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Embodiment

[0027] Refer to attached Figure 1 to Figure 4 , this embodiment provides a method for rapidly separating and purifying the anti-tumor protein components of Nine-scented Worms, including:

[0028] Extraction of hemolymph from Nine-scented worm: Collect Nine-scented worm adults in the field and bring them back to the laboratory, refrigerate them to death, break off their head and wings together, put the rest into an inner centrifuge tube with a hole at the bottom, insert the inner centrifuge tube into the outer centrifuge tube; after centrifugation, the hemolymph in the worm body is separated into the outer centrifuge tube, thereby obtaining the hemolymph and worm body of the worm; In a centrifuge tube of L PMSF, centrifuge at 12000r / min at 4°C for 10min, absorb the water-soluble component of hemolymph into a new centrifuge tube to obtain the water-soluble component of hemolymph, detect the protein content by Bradford, freeze-dry, and use Prepare PBS to the corresponding conce...

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Abstract

The invention provides a method for fast separating and purifying aspongopus protein components resistant to tumors and belongs to the technical field of biology. Through high-temperature treatment, ammonium sulfate chromatography protein and ultrafiltration, fast separation and purification of the aspongopus protein components resistant to tumors are achieved in order to solve the problems that existing methods are cumbersome in procedure and high in requirement for instruments, the price of related chromatographic columns and fillers is high since high performance liquid chromatographs, nucleic acid protein chromatographs and the like are often required for separation and purification, and the purification period is pretty long.

Description

technical field [0001] The invention provides a method for rapid separation and purification of anti-tumor protein components of jiuxiang worm, which belongs to the field of biotechnology. technical background [0002] Aspongopus chinensis Dallas (Aspongopus chinensis Dallas) is an insect of the family Hemiptera of the Insecta order Hemiptera. . It has attracted much attention because of its functions of regulating qi and relieving pain, warming the middle and supporting yang, and containing medicinal ingredients such as anticancer, antibacterial, and anticoagulant. However, its anticancer medicinal ingredients are still unclear. The situation of "knowing what it is, but not knowing why" is a serious hindrance to its being paid attention to by the international academic circles, and it is a shackle for the deep development and utilization of Jiuxiangworm resources. Carry out more in-depth research to provide a basis for the research and development and application of Jiuxi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/34C07K1/30A61P35/00
CPCC07K1/145C07K1/303C07K1/34
Inventor 郭建军檀军田莹曹米兰吴有芳赵帅张书琪
Owner GUIZHOU UNIV
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