Preparation method of high-purity spirulina blue
A high-purity phycocyanin technology, applied in the field of preparation of high-purity phycocyanin, can solve the problems of phycocyanin decomposition, cumbersome operation, and long cycle, and achieve the effects of mild purification conditions, simple preparation process, and low cost
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Examples
Embodiment 1
[0031] Put 300Kg of dry high-quality spirulina in a stainless steel stirring tank, add 2% sodium chloride solution according to the mass ratio of spirulina:salt solution=1:20, and stir at a low speed of 25°C for 7 hours. The dissolved feed liquid is transported to a plate and frame filter press through a pipeline, and the feed liquid is separated by the plate and frame filter press to obtain a crude extract of phycocyanin. Collect the crude phycocyanin extract, add food-grade powdered ammonium sulfate solid to it, until the saturation of ammonium sulfate in the mixed solution is 10%, then adjust the pH to 6.0, stir and mix at 10°C and let stand for 6h, remove The supernatant is diluted with pure water with a conductivity of 1 to 60 us, and then the impurity is removed by a 0.45 μm fine filter, and the filtered feed liquid is desalted and concentrated through an ultrafiltration membrane device with a molecular weight of 30,000 until When the conductivity of the permeate is belo...
Embodiment 2
[0033] Put 300Kg of dry high-quality spirulina in a stainless steel stirring tank, add 1% potassium chloride solution according to the mass ratio of spirulina:salt solution=1:35, and stir at a low speed of 10°C for 6 hours. The dissolved feed liquid is transported to a plate and frame filter press through a pipeline, and the feed liquid is separated by the plate and frame filter press to obtain a crude extract of phycocyanin. Collect the crude phycocyanin extract, add food-grade powdered ammonium sulfate solid to it, until the saturation of ammonium sulfate in the mixed solution is 12%, then adjust the pH to 6.2, stir and mix at 20°C and let it stand for 6h, remove The supernatant is diluted with pure water with a conductivity of 1 to 60us, and then the impurity is removed by a 0.3μm fine filter, and the filtered feed liquid is desalted and concentrated through an ultrafiltration membrane device with a molecular weight of 20,000 until When the conductivity of the liquid is bel...
Embodiment 3
[0035] Put 300Kg of dry high-quality spirulina in a stainless steel mixing tank, add 2% sodium dihydrogen phosphate solution according to the mass ratio of spirulina:salt solution = 1:50, and stir at a low speed of 30°C for 8 hours. The dissolved feed liquid is transported to a plate and frame filter press through a pipeline, and the feed liquid is separated by the plate and frame filter press to obtain a crude extract of phycocyanin. Collect the crude extract of phycocyanin, add food-grade powdered ammonium sulfate solid to it, until the saturation of ammonium sulfate in the mixed solution is 15%, then adjust the pH to 6.5, stir and mix at 30°C and let stand for 6h, remove The supernatant is diluted with pure water with a conductivity of 1 to 60 us, and then the impurity is removed by a 0.22 μm fine filter, and the filtered feed liquid is desalted and concentrated through an ultrafiltration membrane device with a molecular weight of 10,000 until it is transparent When the con...
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Description
Claims
Application Information
- IPC
- C07K14/795; C07K1/14
- CPC
- C07K14/795
- Inventors
- 张军兵; 熊勇