50results about How to "Mild purification conditions" patented technology

The invention belongs to the technical field of bacterial antigen, and discloses a pseudomonas aeruginosa vaccine recombinant protein SBP, and a preparation method and applications thereof. The nucleotide sequence is SEQ ID NO:1; the amino acid sequence is SEQ ID NO:2; a recombinant expression carrier contains a nucleotide sequence SEQ ID NO:1. According to the preparation method, pGEX-6p-1carrieris adopted in construction of a recombinant expression plasmid for expression of recombinant protein SBP; pGEX is an expression fusion protein carrier constructed by Smith and Johnson in 1987, and the main characteristic is that the carrier is grafted with glutathione-S-transferase (GST) with a molecular weight of 26kDa; the expressed fusion protein contains a GST label; and the label is a protein purification label. Compared with other fusion carriers, the pGEX carrier is capable of maintaining the space conformation and the immunogenicity of the purified protein as far as possible.

The invention provides a pseudomonas aeruginosa recombinant protein Vac11 as well as a preparation method and an application. Animal tests prove that the recombinant protein can effectively stimulate organisms to carry out higher humoral immune response and give play to good immunoprotection effects, thus being beneficial to prevention, diagnosis and treatment of pseudomonas aeruginosa. The recombinant protein prepared by adopting the method provided by the invention has high expression quantity and is convenient to separate and purify.

The invention provides PA (pseudomonas aeruginosa) recombinant protein POP, as well as a preparation method and an application thereof. The recombinant protein is formed by sequential connection of a PA III type secretory system component protein PcrV fragment, a PA outer membrane lipoprotein OprI fragment and a PA IV type pilin PilA fragment through a linker. The recombinant protein has a general formula: PcrV fragment-(Linker A)m-Oprl fragment-(Linker B)n-PilA fragment, wherein sequences of the Linker A and the Linker B are respectively independently selected from one of GGGGS, GGSGG and YAPVDV; values allowed for m and n are 1, 2, 3 and 4 independently and respectively. The invention also provides the preparation method and the application of the recombinant protein. Subunit vaccine prepared from the recombinant protein can stimulate the organism to generate high-titer IgG antibodies; the recombinant protein can be used for preparing diagnostic drugs, preventive drugs or therapeutic drugs.

The invention discloses a preparation method of high-purity spirulina blue. The preparation method is characterized by adopting spirulina powder as a raw material, and performing continuous productionincluding extraction, filtration, salting out, desalination concentration, micro-encapsulation, spray drying and mixing to obtain spirulina blue, which is blue powder. Phycocyanin is dark blue powder, is separated from spirulina, and looks beautiful. The main component of spirulina blue is phycocyanin, which belongs to porphyrin cyanophycin and is one of infrequent chromoproteins in the nature. The phycocyanin has bright color, is protein itself with abundant nutrients, has complete amino acid composition, and is high in amino acid content. The preparation method is short in production periodand doesn't cause secondary pollution. The obtained spirulina blue is high in purity, contains 71.3% of phycocyanin, has the purity A620 / A280 reach 3.6, and can meet the requirement of food and cosmetic industry.

The invention discloses a method for preparing high-purity cyclic hexapeptide compound by utilizing Coleophoma empetri to ferment a metabolite. The method comprises the steps of conducting filtration on FR179642 fermentation broth, concentrating the filter liquor, and through the steps of adsorption by macroporous adsorption resin, desorption, desorption solution decoloration, lower temperature crystallization and the like, the high-purity cyclic hexapeptide compound FR179642 product is obtained. The high-purity cyclic hexapeptide compound FR179642 has the advantages that the high-purity cyclic hexapeptide compound FR179642 is extracted by adopting macroporous adsorption resin and combining a method of using a decolorising agent and crystallization, the technology is easy to operate and applicable to industrial production.

The invention discloses a methicillin-resistant staphylococcus aureus (MRSA) vaccine recombinant protein antigen FnbA1 and a preparation method and application thereof. The invention also discloses a method for establishing an expression vector of the recombinant protein and converting host bacteria to prepare the recombinant protein and application of the recombinant protein in preparing a subunit vaccine resistant to MRSA and a related detection kit. Through the invention, the recombinant protein prepared by the method is convenient to separate and purify and efficient and safe, and the expression amount is large. Animal experiments prove that the prepared recombinant protein can effectively stimulate the organism to generate relatively high humoral immune response and good immune protection effect.

The invention relates to a method for extracting and separating phenolic compounds of magnolia through supercritical CO2 liquid technology. The method comprises the following steps that: magnolia is pulverized into dry powders with 20 to 60 meshes; the dry powders are thrown into an extraction kettle; the extraction kettle, a resolution kettle I and a resolution kettle II are heated respectively; carbon dioxide is injected to the extraction kettle and two resolution kettles through a high-pressure pump; the flow speed of the carbon dioxide liquid and the flow speed of an entrainer are adjusted to begin extraction, and the constant temperature and pressure and the stability of a system are kept for circular extraction; after every circulation for thirty minutes, the materials are discharged from the resolution kettle I and the resolution kettle II to obtain a flaxen extractant; the flaxen extractant obtained by the resolution kettle I and the resolution kettle II is dissolved by petroleum ether in proper amount, kept stand and filtered; the petroleum ether is reclaimed from the filtrate; the filtrate is recrystallized by a recrystallization solvent to obtain magnolol and mixed total phenol of the magnolol; the mixed total phenol is recrystallized to obtain a magnolol crystal; and after a mother solution is concentrated, the mother solution is recrystallized by the recrystallization solvent to obtain the magnolol crystal.

The invention discloses a preparation method of high-purity spirulina blue. The preparation method is characterized by adopting spirulina powder as a raw material, and performing continuous productionincluding extraction, filtration, salting out, desalination concentration, micro-encapsulation, spray drying and mixing to obtain spirulina blue, which is blue powder. Phycocyanin is dark blue powder, is separated from spirulina, and looks beautiful. The main component of spirulina blue is phycocyanin, which belongs to porphyrin cyanophycin and is one of infrequent chromoproteins in the nature. The phycocyanin has bright color, is protein itself with abundant nutrients, has complete amino acid composition, and is high in amino acid content. The preparation method is short in production periodand doesn't cause secondary pollution. The obtained spirulina blue is high in purity, contains 71.3% of phycocyanin, has the purity A620 / A280 reach 3.6, and can meet the requirement of food and cosmetic industry.

The invention provides a method for preparing aluminum sulfate from an aluminum-containing acid treatment solution, the method comprising: (1) gelling the aluminum-containing acid treatment solution, and filtering and separating to obtain a gel and a gel filtrate; (2) Add concentrated sulfuric acid in the gel filtrate, once salting out crystallization obtains thick aluminum sulfate; (3) thick aluminum sulfate is dissolved in water, filters to obtain calcium sulfate and decalcification filtrate; (4) adds concentrated sulfuric acid in decalcification filtrate, two High-purity aluminum sulfate is obtained by secondary salting-out crystallization; (5) evaporation and concentration of the secondary salting-out crystallization filtrate is obtained to obtain high-purity sodium sulfate precipitate. The invention develops a comprehensive treatment and resource utilization method for the acid treatment waste liquid of minerals, especially the acid treatment waste liquid of the coal acid-base ash removal method. With this technology, alumina can be effectively extracted and aluminum sulfate can be prepared to meet the needs of the market. It has very good industrial application value.

The invention relates to an A1S-1462 recombinant protein and a preparation method and application thereof. The recombinant protein comprises A1S-1462 mature peptide, and the amino acid sequence of the recombinant protein is shown as SEQ ID NO. 3. The recombinant protein is high in expression amount, easy to separate and purify, efficient and safe, can be directly used with an adjuvant, and is used for the preparation of an acinetobacter baumannii infection resistant subunit vaccine and a related detection kit. Confirmed by animal experiments, the genetic engineering recombinant subunit vaccine has good acinetobacter baumannii infection resistant immune protection effect, lays a foundation for the further study on combined vaccines and multicomponent fusion vaccines, and plays an important role for development and application of prevention and control vaccines and diagnostic kits.

The invention belongs to the technical field of bacterial antigens, and discloses a recombinant protein SBP of Pseudomonas aeruginosa vaccine and its preparation method and application; the nucleotide sequence is SEQ ID NO: 1; the amino acid sequence is SEQ ID NO: 2; the recombinant The expression vector comprises the nucleotide sequence of SEQ ID NO:1. The present invention adopts pGEX-6p-1 carrier to construct recombinant expression plasmid, expresses recombinant protein SBP, pGEX is the carrier expressing fusion protein constructed by Smith and Johnson in 1987, and its main feature is that the carrier is connected with a molecular weight of 26kDa Glutathione‑S‑transferase (GST), the expressed fusion protein contains a GST tag, which is a marker for protein purification. Compared with other fusion vectors, the pGEX series vectors can keep the purified protein in its spatial conformation and immunogenicity to the greatest extent.

The invention discloses a preparation method of four kinds of blood-derived proteins. The purification method combined with anion / cation exchange chromatography and affinity chromatography is used to simultaneously purify α1-antitrypsin (AAT), transgenic Ferritin (TRF), α1-acid glycoprotein (AAG) and haptoglobin (HP) four proteins, the preparation method disclosed by the present invention is simple to operate, the obtained target protein has stable activity, high purity and high yield, suitable for For industrial production and clinical applications.

The invention relates to a system and method for extracting sulfur from sulfur-containing foam in a coking plant. The system includes an extraction unit, a separation unit and a post-processing unit; the extraction unit includes an extraction tower, and the extraction tower includes a tower body and a motor. The tower body is from top to bottom. The lower section includes an upper clarification section, a mixing section and a lower clarification section in turn. The upper clarification section is provided with a raffinate phase outlet, the lower clarification section is provided with an extraction phase outlet, and the top and bottom of the mixing section are respectively provided with an extractant inlet. and a sulfur-containing foam feed port; the separation unit includes a thermal insulation filter device, and the feed port is connected to the extraction phase discharge port; the post-processing unit includes an evaporative crystallizer, and the still body of the evaporative crystallizer is provided with a material inlet, and a vapor phase outlet The material inlet is connected with the material outlet, the material inlet is connected with the filtrate outlet of the thermal insulation filter device, the vapor phase outlet is connected with the extractant inlet of the extraction tower, and the material outlet is used to obtain sulfur. The invention directly separates useful components such as sulfur and ammonium salt from sulfur-containing foam, and is economical and environment-friendly.

The invention provides a method for preparing the high-purity oritavancin key intermediate A82846B, specifically by separating the oritavancin key intermediate A82846B from the fermentation broth, first adjusting the pH, and then purifying the oritavancin key intermediate through a macroporous adsorption resin , then purified by reverse phase chromatography, and finally crystallized to obtain high-purity A82846B. The method adopted in the present invention is simple to operate, uses less organic solvents, and greatly reduces the generation of waste liquid; and overcomes the defects of low adsorption efficiency of cationic macroporous adsorption resins in the prior art and reduced product yield due to leakage adsorption, and is suitable for Industrial production.

The invention discloses a recombinant fusion protein HF2 which is composed of staphylococcus aureus alpha hemolysin (H1a) and a fibronectin binding protein (FNBA) segment, wherein the H1a and the FNBA are fused through a linker peptide. The protein can be used for preparing subunit vaccines and relevant detection kits capable of resisting infections of the methicillin-resistant staphylococcus aureus (MRSA). A gene engineering technology is used to recombine and express the fused protein. The method has high expression level, facilitates separation and purification and has high efficiency and safety. Animal experiment results show that the recombinant fusion protein HF2 can effectively stimulate a body to produce relatively high humoral immune response and good immune protection effect.

The invention provides PA (pseudomonas aeruginosa) recombinant protein POP, as well as a preparation method and an application thereof. The recombinant protein is formed by sequential connection of a PA III type secretory system component protein PcrV fragment, a PA outer membrane lipoprotein OprI fragment and a PA IV type pilin PilA fragment through a linker. The recombinant protein has a general formula: PcrV fragment-(Linker A)m-Oprl fragment-(Linker B)n-PilA fragment, wherein sequences of the Linker A and the Linker B are respectively independently selected from one of GGGGS, GGSGG and YAPVDV; values allowed for m and n are 1, 2, 3 and 4 independently and respectively. The invention also provides the preparation method and the application of the recombinant protein. Subunit vaccine prepared from the recombinant protein can stimulate the organism to generate high-titer IgG antibodies; the recombinant protein can be used for preparing diagnostic drugs, preventive drugs or therapeutic drugs.

The present invention relates to a recombinant protein of 1A1S_1969 and its preparation method and application. The recombinant protein comprises the 46-414th amino acid sequence of A1S_1969. The amino acid sequence of the recombinant protein is shown in SEQ ID NO.5. The recombinant protein has a high expression level, is convenient for separation and purification, is highly efficient and safe, and can be directly used in conjunction with an adjuvant to prepare subunit vaccines against Acinetobacter baumannii infection and related detection kits. It is confirmed by animal experiments that the genetically engineered recombinant monovalent subunit vaccine has a good immune protection effect against Acinetobacter baumannii infection. It lays the foundation for further research on combined vaccines and multi-subunit fusion vaccines, and plays an important role in the development and application of prevention and control vaccines and diagnostic kits.