Purification and separation method of phenolic compounds in Magnolia officinalis

Abstract

The invention relates to a method for extracting and separating phenolic compounds of magnolia through supercritical CO2 liquid technology. The method comprises the following steps that: magnolia is pulverized into dry powders with 20 to 60 meshes; the dry powders are thrown into an extraction kettle; the extraction kettle, a resolution kettle I and a resolution kettle II are heated respectively; carbon dioxide is injected to the extraction kettle and two resolution kettles through a high-pressure pump; the flow speed of the carbon dioxide liquid and the flow speed of an entrainer are adjusted to begin extraction, and the constant temperature and pressure and the stability of a system are kept for circular extraction; after every circulation for thirty minutes, the materials are discharged from the resolution kettle I and the resolution kettle II to obtain a flaxen extractant; the flaxen extractant obtained by the resolution kettle I and the resolution kettle II is dissolved by petroleum ether in proper amount, kept stand and filtered; the petroleum ether is reclaimed from the filtrate; the filtrate is recrystallized by a recrystallization solvent to obtain magnolol and mixed total phenol of the magnolol; the mixed total phenol is recrystallized to obtain a magnolol crystal; and after a mother solution is concentrated, the mother solution is recrystallized by the recrystallization solvent to obtain the magnolol crystal.

Description

technical field [0001] The present invention relates to the extraction and purification separation method of phenolic compound in Magnolia officinalis medical material, especially relate to using supercritical CO 2 (SFE-CO 2 ) Fluid technology is a method for extracting and separating phenolic compounds in Magnolia officinalis such as Magnolia officinalis and Honokiol from Magnolia officinalis medicinal materials. Background technique [0002] Magnolol (Magnolol) and honokiol (Hokolol) are the main chemical constituents of the traditional Chinese medicine Magnolia officinalis. Magnolol and honokiol belong to biphenol compounds, and their molecular formulas are shown in the figure below. With the development of modern medicine in recent years, it has been found that magnolol and honokiol mainly have the following medicinal effects: (1) warming the middle and drying dampness: it is used for abdominal distension, vomiting and diarrhea caused by dampness and turbidity. (2) Pr...

AI Technical Summary

Problems solved by technology

[0006] The traditional technology mainly uses a large amount of solvent extraction and recrystallization method. The extraction liquid of this separation method contains too many impurities, the separation is relatively difficult, and a large number of organic solvents must be used many times. The number of process steps is long, the cost is too high, and the extraction rate Not very high, not suitable for industrial production

The alkaline extraction method is easy to oxidize phenolic substances such as magnolol

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