Acinetobacter baumanniihy pothetical protein A1S-1462 protein and preparation method and application thereof

Abstract

The invention relates to an A1S-1462 recombinant protein and a preparation method and application thereof. The recombinant protein comprises A1S-1462 mature peptide, and the amino acid sequence of the recombinant protein is shown as SEQ ID NO. 3. The recombinant protein is high in expression amount, easy to separate and purify, efficient and safe, can be directly used with an adjuvant, and is used for the preparation of an acinetobacter baumannii infection resistant subunit vaccine and a related detection kit. Confirmed by animal experiments, the genetic engineering recombinant subunit vaccine has good acinetobacter baumannii infection resistant immune protection effect, lays a foundation for the further study on combined vaccines and multicomponent fusion vaccines, and plays an important role for development and application of prevention and control vaccines and diagnostic kits.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to the hypothetical protein A1S_1462 protein of Acinetobacter baumannii and its preparation method and application. Background technique

[0002] Acinetobacter baumannii (Acinetobacter baumannii) is a non-fermenting gram-negative bacillus, which widely exists in nature and belongs to conditional pathogenic bacteria. This bacterium is an important pathogen of nosocomial infection, mainly causing respiratory tract infection, and can also cause bacteremia, urinary tract infection, secondary meningitis, surgical site infection, ventilator-associated pneumonia, etc. Domestic data show that Acinetobacter baumannii accounts for more than 70% of clinically isolated Acinetobacter. The resistance rate of Acinetobacter baumannii to the third and fourth generation cephalosporins has reached 63.0% to 89.9%. The resistance rate to four aminoglycosides (amikacin, gentamicin, netilmicin, tobramycin) ...

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