35results about How to "Quality and safety controllable" patented technology

The present invention relates to a method for preparing enzyme powder, and the method is characterized in that a blueberry enzyme is prepared by using byproducts, such as blueberry pomaces or blueberry wine dregs, in the blueberry processing industry. The method comprises treatment processes of pretreatment, enzymolysis, fermentation, drying, etc. The enzyme powder prepared by the method not only has abundant proteases, lipases, amylases, cellulases, superoxide dismutase, metabolites and a large number of probiotic cells, but also has health care functions of blueberries. The preparation method shortens the fermentation time of the enzyme to several weeks via employing a modern pure-strain mixed fermentation technology, compared with conventional fermentation methods of which the fermentation time ranges from several months to 1 year. The enzyme prepared by the method has a higher nutritional value (the active enzyme content is higher than that of conventional fermentation methods) and a stable quality. The enzyme powder fills gaps in the prior art, and has higher nutritional values and health-care efficacy. The enzyme powder has medical values and health care functions after long-term consumption, such as, expelling toxins and beautifying, softening blood vessels, improving microcirculations, improving antioxidant capacity of a body, deferring senescence, etc.

The invention discloses a ballast track laser intelligent track lifting and lining system. The ballast track laser intelligent track lifting and lining system comprises a track lifting and lining tamper (1) and is characterized by further comprising an integrated full-route measurement device, the full-route measurement device comprises a laser transmitting device (4) and an image receiving device (5), the image receiving device (5) is arranged on a front end image receiving trolley (7), and the laser transmitting device (4) is arranged on a laser transmitting movable trolley (2) in a distance. The image receiving device (5) is provided with a two-dimensional automatic tracking mechanism. A construction method includes the steps that route geometrical shape parameters are input, and track lifting and lining are conducted; each time the ballast track laser intelligent track lifting and lining system advances by a set distance, an electrical control system collects front end lifting and lining quantity data, a gva system outputs track lifting and lining quantity data control operation of an operation position, and the gva system feeds back track lifting and lining quantity data of an operation process. By means of the ballast track laser intelligent track lifting and lining system and the construction method, ballast track accurate track lifting and lining measurement workloads are greatly reduced, construction accuracy is improved, the advantages of the full-route measurement system and a large track maintenance machine are given into full play, and the problem of man-made errors occurring in the connection between the measurement procedure and the track lifting and lining construction procedure is thoroughly solved.

The invention discloses a fatigue-relieving traditional Chinese medicine health product, which is composed of the following seven traditional Chinese herbals: ginseng, processed rhizoma polygonati, wolfberry, raspberry, maca, epimedium, and cervus elaphus antler. The pharmacodynamic test result show that the health product can prolong the load swinging time of mice, the contents of hepatic glycogen and lactic dehydrogenase are increased, the contents of serum urea nitrogen and blood lactic acid are reduced, and the health product has a prominent fatigue relieving effect.

The invention relates to an acinetobacter baumannii A1S-1610 protein, a carrier, engineering bacteria, a composition or a kit which comprises the acinetobacter baumannii 1 A1S-1610 protein, and a preparation method and application of the acinetobacter baumannii 1 A1S-1610 protein, the acinetobacter baumannii 1 A1S-1610 protein is never used in the field of recombinant subunit vaccines, the acinetobacter baumannii 1 A1S-1610 protein can effectively stimulate the body to cause a protective immune response so as to resist acinetobacter baumannii lethal infection. The present invention also discloses a method for preparation of the recombinant protein by construction of an expression vector of the recombinant protein, and transformation of host bacteria, and use of the recombinant protein in the preparation of an acinetobacter baumannii resistant subunit vaccine and a related detection kit. The protective antigen composition A1S_1610 protein is expressed by use genetic engineering technology to clone, and the A1S_1610 protein is high in expression amount, easy to separate and purify, efficient and safe, and the genetic engineering recombinant subunit vaccine has good acinetobacter baumannii infection resistant immune protection effect.

The invention provides a fully-assembled steel-structure wading basic platform and a basic member thereof and particularly provides the on-site final-assembly construction of the steel-structure wading basic platform and the standardized design and scale production of a basic structural unit, i.e. a steel floating body (1), of the steel-structure wading basic platform. According to the fully-assembled steel-structure wading basic platform, the traditional wading infrastructures are subjected to improvement and innovation in the aspects of structural design and construction manner; the basic structural unit, i.e. the steel floating body (1), is subjected to standardized and definite-form design, so that the basic structural unit becomes a series finished product member which can be subjected to scale and mass production in factory workshops; and the construction of the fully-assembled steel-structure wading basic platform is quickly completed in a manner that steel floating body (1) series finished products are selected as required and are subjected to on-site final-assembly like building-block model building or tetris assembling (referring to a final-assembly diagrammatic drawing of the fully-assembled steel-structure wading basic platform in attached drawings). The fully-assembled steel-structure wading basic platform is applicable to but not limited by two platforms including near-water and on-water platforms of different functions and uses and platforms taking the near-water and on-water platforms as foundations of other on-water constructions, and can be widely applied to wading construction projects, such as ports, wharfs, transportation pontoons, aquaculture cages and on-water dining and entertainment platforms.

The invention provides a method for detecting a lung-heat clearing and cough relieving preparation. Raw materials of the lung-heat clearing and cough relieving preparation include phyllanthus emblica, costustoot and pterocephalus hookeri. The method is characterized in that the phyllanthus emblica, the costustoot and / or the pterocephalus hookeri in the lung-heat clearing and cough relieving preparation are identified by thin-layer chromatography. The method has the advantages of reproducibility, fine stability, simplicity and convenience in operational approach, high precision and specificity, capability of clearly developing spots, good resolution and the like, and meets the principle of accuracy, simplicity, convenience, sensitivity and quickness. By the aid of the established reliable quality detection method with high specificity, the quality of the lung-heat clearing and cough relieving preparation can be effectively controlled and is stable, safe and controllable.

The invention discloses a cartilage graft and a construction method thereof. The cartilage graft is a cartilage membrane or injectable cartilage graft and contains a cartilage membrane or cartilage micro-tissue formed by in-vitro culture of cartilage cells.

The invention relates to the field of genetic engineering, and discloses PAL recombination protein of acinetobacter baumannii and a coding gene and application of the PAL recombination protein. The PAL recombination protein is (a) recombination protein as shown in SEQID NO:1 or SEQID NO:3; and (b) protein which is obtained by substituting, deleting and adding one or more amino acids for the aminoacid sequence as shown in SEQ ID NO:1 or SEQID NO:3, and has the same function as the recombination protein as shown in SEQID NO:1 or SEQID NO:3 and derived from (a) or protein which is prepared by connecting labels to the terminal of amino terminal and / or carboxyl terminal of the SEQID NO:1 or the SEQID NO:3 and shown in the amino acid sequence. The recombination protein is high in expression level, convenient to separate and purify, and high in efficacy and safe, can directly cooperate with an adjuvant for preparing a subunit vaccine and a relevant detection product, for resisting infectionof the acinetobacter baumannii.

The invention relates to an A1S-1462 recombinant protein and a preparation method and application thereof. The recombinant protein comprises A1S-1462 mature peptide, and the amino acid sequence of the recombinant protein is shown as SEQ ID NO. 3. The recombinant protein is high in expression amount, easy to separate and purify, efficient and safe, can be directly used with an adjuvant, and is used for the preparation of an acinetobacter baumannii infection resistant subunit vaccine and a related detection kit. Confirmed by animal experiments, the genetic engineering recombinant subunit vaccine has good acinetobacter baumannii infection resistant immune protection effect, lays a foundation for the further study on combined vaccines and multicomponent fusion vaccines, and plays an important role for development and application of prevention and control vaccines and diagnostic kits.

The invention discloses a medicine composition curing gynaecological mass diseases such as fibroids and a preparation method and application of the medicine composition. The medicine composition mainly comprises the following raw materials: glabrous greenbrier rhizome, lightyellow sophora roots, cortex phellodendri, zedoray rhizome, rhizoma sparganic, roots of red-rooted salvia, red peony roots, szechwan chinaberry fruits, rhizoma corydalis, rhizoma corydalis, chinese angelica, Gorgon fruits and Chinese yams, extraction and purification are carried out through a certain technique, and auxiliary materials and components which are acceptable in the pharmacy are added to prepare the medicine composition. According to the preparation method, volatile components are extracted in a supercritical mode and prepared into micro-capsules to avoid loss of the volatile components, the preservation time limit of the product is promoted, and the stimulus effect on gastrointestinal tracts is reduced. The method is extensive in medicinal herbs resources and free of toxic, strong and tabooed medicines, all the effective components contribute to synergistic effects, the effective substances are clear, the quality safety is controllable, the curative effect is definite, and the medicine composition can be accepted well and is a new effective medicine curing the gynaecological mass diseases such as the fibroids.

The invention belongs to the technical field of pharmacy, and particularly provides a sustained or controlled release solid composition comprising a bupropion hydrochloride framework and a retarding layer. The sustained or controlled release solid composition is characterized in that the solid composition is composed of a table core comprising effective dose of bupropion hydrochloride and a framework sustained release material for controlling drug release, and a water-insoluble retarding layer coating the tablet core. The sustained or controlled release solid composition comprising bupropion hydrochloride, provided by the invention is safe and effective clinically, and is convenient for administration. The preparation method of the sustained or controlled release solid composition is simple, and suitable for industrial production.

The invention discloses a pharmaceutical composition with effect on treating irritable bowel syndrome and a preparation method and an application thereof, and the pharmaceutical composition is prepared by the following raw material herbs by weight: 1-2 parts of red ginseng, 1-2 parts of athlets root skin, 1-2 parts of sargentodoxa vine, 1-2 parts of jujube, and 1-2 parts of coix seeds. The pharmaceutical composition provided by the invention is formulated based on traditional Chinese medicine theory and syndrome differentiation and treatment theory, and clinical experiment results show that the pharmaceutical composition provided by the invention has good effect on treating irritable bowel syndrome; extracts of the pharmaceutical composition provided by the invention can be prepared into various dosage forms with pharmaceutical carriers conveniently; the pharmaceutical composition is convenient for administration clinically, and has no toxic or side effect after long-term use.

The invention provides a method for detecting a white vein preparation. The white vein preparation comprises raw material medicines of pepper, liquorice and turmeric, and the method comprises the step of detecting the pepper, the liquorice and / or the turmeric through a thin-layer chromatography method. According to the detection method, the pepper, the liquorice and the turmeric in the prescription are detected and researched by the thin-layer chromatography method, the established thin-layer chromatography method has the advantages of high reproducibility, high stability, easy and convenient operation method, high precision, high specificity, clear spot color development, high degree of separation and the like; as a reliable quality detection method with high specificity is established, the quality of the white vein preparation can be effectively controlled; and the quality of the white vein preparation can be stable, safe and controllable.

The invention discloses a rose extraction method which comprises the following steps: weighing, drying and crushing rose, carrying out percolation for 3-4 h by 90-95% ethyl alcohol, wherein the PH of ethyl alcohol is adjusted to 4-5 by refined hydrochloric acid, taking ethyl alcohol filtrate, reserving herb residues, and recycling ethyl alcohol from filtrate at reduced pressure until the filtrate is free of alcohol smell so as to obtain a concentrated solution 1; adding water, the use amount of which is eight times of that of the rose residues, into the rose residues, boiling for 1 hour, extracting for two times, mixing filtrates, and cooling the mixed filtrate to 40 DEG C so as to obtain filtrate 2; enabling the filtrate 2 to be adsorbed on a chelating resin column, replacing material liquid in the resin with a 7% sodium chloride solution after filtrate 2 is completely adsorbed on the column, collecting effluent and recycling at reduced pressure so as to obtain a concentrated solution 2, wherein the specific gravity is controlled to be 1.08-1.11; and after the concentrated solution 1 and the concentrated solution 2 are mixed, spray-drying so as to obtain a rose extractive. The rose extractive extracted through the extraction method provided by the invention has the characteristics that storage and transportation are convenient, heavy metal content is low, quality is safe and controllable, colour, luster and fragrance of rose are retained to the greatest extent, and effective ingredients are extracted sufficiently.

The invention relates to the technical field of drug detection, and particularly discloses a method for detecting a residual solvent in antiangitide by using a gas chromatographic method and application. According to the method for detecting the residual solvent in antiangitide by utilizing the gas chromatographic method, the residual solvent is dimethyl sulfoxide, a direct sample injection mode is adopted, and a programmed heating mode is as follows: the initial temperature is 50-60 DEG C and is kept for 1-2 minutes; and heating is performed to 210-220 DEG C at the heating rate of 20 DEG C / min, the temperature is kept for 4-6 minutes, and the process is finished after 13-16 minutes. The gas chromatographic detection method is high in sensitivity and good in specificity, stability and accuracy, the quality of antiangitide can be effectively controlled, and the quality of antiangitide is stable, controllable and safe.

The invention discloses an extract prepared by extracting from longan leaves and having the activity of inhibiting alpha-glucosidase. The extract can be obtained by pulverizing the longan leaves and then performing ultrasonic extracting with ethanol, an ethanol extract is decompressed to recover ethanol, and then an extract is obtained by concentration, and the extract is suspended by adding water, the petroleum ether and ethyl acetate are used for extraction in order to obtain an ethyl acetate extract; and after extraction with the ethyl acetate, n-butanol is used for extraction to obtain then-butanol extract. Pharmacological experiments show that the effective part has a good inhibition of alpha-glucosidase activity, and the effect of ethyl acetate extract is more obvious. The method adopts the abundant and low-priced longan leaves as a raw material, and provides a wider source of diabetes therapeutic drugs, has good social value and economic value, and plays an important role in developing an efficient and safe hypoglycemic new drug.

The invention relates to a purification method of anidulafungin. The purification method of anidulafungin comprises the steps of 1, dissolving and distaining, wherein, an anidulafungin crude product into a crystal solvent is added to make the solid completely dissolve, activated carbon is added to distain by stirring, and the dissolved solution is obtained after discoloring by filtering; 2, sampling the anidulafungin dissolved solution, loading the sample in macroporous adsorption resin, and increasing the pressure to wash the resin column with an acid or neutral water solution under the condition of 3-9bar; 3, concentrating, wherein, the eluent is concentrated until the eluent is dry, so that an anidulafungin pure product with content of more than 99.5% is obtained. The purification method of the anidulafungin is simple in technology, convenient to operate, and less in adopted amount of solution, and the isomer impurities with similar structural properties can be effectively removed. The product is high in purity and safe and controllable in quality, can be applicable to a refining method of the anidulafungin for industrial production, and the purity can be increased to 99.5% or above.

The invention discloses a preparation method of anti-senility immune enhancement concentrated ginseng calcium peptide. The anti-senility immune enhancement concentrated ginseng calcium peptide is prepared from American ginseng, scarlet caterpillar fungus, male silkworm moth and other raw materials; the other raw materials are treated by the following steps: 1, separately pulverizing the raw materials, extracting, filtering for the first time, and taking out a soak solution; 2, distilling the soak solution which is obtained by filtering for the first time in the step 1; 3, dissolving and stirring; 4, filtering the mixture, subjected to dissolving and stirring, for the second time to obtain alcohol mixed liquid; 5, distilling the alcohol mixed liquid to remove alcohol, and drying the residuewithout the alcohol to obtain the raw materials which are qualified after subjected to treatment; the finished product is obtained by evenly mixing the raw materials, which are qualified after subjected to treatment, according to a certain ratio, and then preparing into a preparation. The anti-senility immune enhancement concentrated ginseng calcium peptide can improve the immunologic function ofa human body, deeply mobilize the activity of daily living of the human body and relieve mental fatigue, and is clear in ingredients as well as safe and controllable in quality, thus being suitable for long-term use.

The invention discloses a traditional Chinese medicine health care product with anti-fatigue function, which is made of ginseng, Rhizoma Polygonatum, wolfberry fruit, raspberry, maca, epimedium and red deer antler. It prolongs the weight-bearing swimming time of mice, increases the content of liver glycogen and lactate dehydrogenase, reduces the content of serum urea nitrogen and blood lactic acid, and has a significant anti-fatigue effect.

The invention discloses a clinical-grade serum-free culture medium for stem cells. According to the culture medium, an L-DMEM culture medium, an IMDM culture medium, a DMEM-F12 or alpha-MEM culture medium, an mTeSRTM1 culture medium, an E8 culture medium, a KSR conditioned culture medium and the like are used as basic culture media, and lipid substances such as inositol and linoleic acid and synthetic lipid substance components are removed; and meanwhile the culture medium contains the following added components: polyvinyl alcohol, bone morphogenetic protein BMP-4, vitamin C, retinoic acid, nicotinamide and azithromycin. The culture medium is clear in composition, controllable in quality and free of animal serum components, can be used for culturing various stem cells including pluripotent stem cells and multipotent stem cells, and can maintain the capability of stemness of the stem cells without differentiation after multiple passages.

The invention relates to an A1S-1462 recombinant protein and a preparation method and application thereof. The recombinant protein comprises A1S-1462 mature peptide, and the amino acid sequence of the recombinant protein is shown as SEQ ID NO. 3. The recombinant protein is high in expression amount, easy to separate and purify, efficient and safe, can be directly used with an adjuvant, and is used for the preparation of an acinetobacter baumannii infection resistant subunit vaccine and a related detection kit. Confirmed by animal experiments, the genetic engineering recombinant subunit vaccine has good acinetobacter baumannii infection resistant immune protection effect, lays a foundation for the further study on combined vaccines and multicomponent fusion vaccines, and plays an important role for development and application of prevention and control vaccines and diagnostic kits.

The invention discloses a serum-free culture medium for clinical grade stem cells. The culture medium of the present invention is composed of L-DMEM, IMDM, DMEM-F12 or α-MEM, mTeSR TM 1 Medium, E8 medium and KSR conditioned medium are basal medium, remove lipids such as inositol and linoleic acid and synthetic lipids, and also contain the following additives: polyvinyl alcohol, bone morphogenetic protein BMP‑4, Vitamin C, Retinoic Acid, Niacinamide, Azithromycin. The medium has clear components, controllable quality, and does not contain animal serum components. It can cultivate various stem cells including pluripotent stem cells and multipotent stem cells, and can maintain the "stemness" of stem cells without the ability to differentiate after multiple passages.

The invention relates to a refined sweetsop total lactone for anticancer and a method for preparing the same. The method comprises that: Root, leaf and bark or seed of sweetsop are used as raw materials, are percolated and extracted by petroleum ether, chloroform, and acetic ether, or a mixed solution of the petroleum ether, the chloroform and acetic ether, or the raw materials are extracted by supercritical carbon dioxide; the raw materials adopts solvent method, namely the raw materials are heated and dissolved by the petroleum ether and are subjected to impurity removal to obtain sweetsop total lactone; and the sweetsop total lactone is subjected to silica gel column chromatography, is subjected to gradient elution by the petroleum ether-the acetic ether or the chloroform-methanol, andis separated and is refined to obtain the sweetsop total lactone taking Shikemoxin and valeric sweetsop as main compositions. The raw materials have rich sources, low price and availability; the obtained refined sweetsop total lactone has high content, clear compositions and controllable quality; and the preparation method has a simple process, low consumption and high yield, and can realize large industrialized production.

The invention discloses a taxus chinensis extractive with blood sugar reduction effect. The taxus chinensis extractive is extracted from taxus chinensis plant branches and leaves by using modern separation methods. The content of refined biflavone in an effective part can be 50-80%; the main effective components are umbrella pine biflavone, ginkgetin, isoginkgetin, ginkgetin biflavone and amentoflavon. Experimental research shows that the taxus chinensis extractive can not only remarkably suppress the alpha-glucosidase activity, but also has an outstanding effect of reducing the rat blood sugar of diabetes, can relieve or suppress happening and development of diabetic complication, and has the hope to be developed into novel blood sugar reduction medicines.

The invention relates to a pharmaceutical composition of aminopyrimidine compounds and a preparation method thereof. Specifically, the pharmaceutical composition comprises 4-[(4-methyl-1-piperazinyl)methyl]-N-[6-methyl-5-[(4-(3-pyridyl)-2-pyrimidinyl)amino]pyridine-3-yl]-3-(trifluoromethyl)-benzamide, a polymorph, a solvate, a hydrate,pharmaceutically acceptable salt or a combination thereof whichare used as the active components, and at least a pharmaceutically acceptable accessory. The composition has good stability and drug dissolution; product quality is controllable; and the preparationmethod has simple process and strong operationality, and is suitable for industrial mass production.

The invention relates to an acinetobacter baumannii A1S-1610 protein, a carrier, engineering bacteria, a composition or a kit which comprises the acinetobacter baumannii 1 A1S-1610 protein, and a preparation method and application of the acinetobacter baumannii 1 A1S-1610 protein, the acinetobacter baumannii 1 A1S-1610 protein is never used in the field of recombinant subunit vaccines, the acinetobacter baumannii 1 A1S-1610 protein can effectively stimulate the body to cause a protective immune response so as to resist acinetobacter baumannii lethal infection. The present invention also discloses a method for preparation of the recombinant protein by construction of an expression vector of the recombinant protein, and transformation of host bacteria, and use of the recombinant protein in the preparation of an acinetobacter baumannii resistant subunit vaccine and a related detection kit. The protective antigen composition A1S_1610 protein is expressed by use genetic engineering technology to clone, and the A1S_1610 protein is high in expression amount, easy to separate and purify, efficient and safe, and the genetic engineering recombinant subunit vaccine has good acinetobacter baumannii infection resistant immune protection effect.

The invention provides a traditional Chinese medicine composition for treating sleeping disorder and its preparation method and use. The traditional Chinese medicine composition is prepared from 4-8 parts by weight of spine date seed, 1.2-3 parts by weight of pilose asiabell root, 1-3 parts by weight of rhizoma pinelliae preparata, 1-3 parts by weight of bitter orange, 1-3 parts by weight of dried orange peel, 1-3 parts by weight of caulis bambusae in taeniis, 1-3 parts by weight of polygala root, 2-5 parts by weight of oyster, 1-3 parts by weight of poria cocos, 1-3 parts by weight of grass-leaved sweetflag rhizome, 1-3 parts by weight of prepared rehmannia root, 2-5 parts by weight of dragon bone and 0.5-2 parts by weight of honey-fried licorice root. Compared with the existing medicine, the traditional Chinese medicine composition has effects of warming bladder and tonifying qi, tranquilizing mind and calming nerve and promoting sleeping, can obviously shorten time for falling asleep and can prolong sleeping time. The traditional Chinese medicine composition has clear curative effects especially on patients suffering from heart, gallbladder and qi deficiency-type sleeping disorder. The preparation method of the traditional Chinese medicine composition has stable processes, can extract a high-content and impurity-less active ingredient, has strong operationality and is suitable for industrial large-scale production.

The present invention relates to a pharmaceutical composition of aminopyrimidine compounds and a preparation method thereof, specifically, the pharmaceutical composition comprises 4-[(4-methyl-1-piperazinyl)methyl]-N-[6-methyl yl-5-[(4-(3-pyridyl)-2-pyrimidinyl)amino]pyridin-3-yl]-3-(trifluoromethyl)-benzamide, its polymorphs, solvates Compounds, hydrates, pharmaceutically acceptable salts or combinations thereof as active ingredients, and at least one pharmaceutically acceptable excipient. The composition has good stability and drug dissolution rate, controllable product quality, simple preparation process, strong operability and is suitable for large-scale industrial production.