Method for detecting ten-flavour rankincense preparation

A detection method, the technology of Shiwei Boswellia, which is applied in the detection of pharmaceutical composition preparations, and the detection field of Shiwei Boswellia preparations, can solve the problem of the inability to realize the controllability of Shiwei Boswellia pills, the poor reproducibility of frankincense and cassia seeds, etc. problem, to achieve the effect of strong specificity, stable quality and high precision

Active Publication Date: 2014-04-23
TIBET QIZHENG TIBETAN MEDICINE
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The reproducibility of the identification of frankincense and cassia seeds in the original standard was not good, and the controllability of the detection method of the quality standard of Shiwei frankincense pills could not be realized, so it was modified on the basis of it; the identification method of frankincense in the original ministerial standard was also made Appropriate revisions have been made to the identification method of cassia seeds in order to provide a method with good reproducibility and strong specificity, which conforms to the principles of accuracy, simplicity, sensitivity and speed, and can effectively control the quality of the product.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting ten-flavour rankincense preparation
  • Method for detecting ten-flavour rankincense preparation
  • Method for detecting ten-flavour rankincense preparation

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0043] Experimental example 1: TLC detection of frankincense

[0044] (1) Instrument

[0045] Mortar, electronic scale, Erlenmeyer flask with stopper, pipette, ultrasonic extractor, funnel, filter paper, evaporating dish, spotter, silica gel G plate, chromatography cylinder, spray bottle, hair dryer.

[0046] (2) Control medicinal materials

[0047] frankincense control medicinal material

[0048] (3) Reagents

[0049] Ethanol, acetone, ether, n-hexane, cyclohexane, ethyl acetate, vanillin, sulfuric acid.

[0050] (4) Inspection method:

[0051] Selection of extraction solvent: use ethanol, acetone, ether and other solutions as extraction solvents;

[0052] Selection of extraction methods: ultrasonic and cold soaking respectively;

[0053] The choice of developer: use n-hexane-ethyl acetate (volume ratio of 9:1), cyclohexane-ethyl acetate (volume ratio of 9:1), cyclohexane-ethyl acetate volume ratio of (6 : 4) is developing agent; Select above extraction solvent, extr...

experiment example 2

[0058] Experimental example 2: TLC detection of woody fragrance

[0059] (1) Instrument

[0060] Mortar, electronic scale, Erlenmeyer flask with stopper, pipette, ultrasonic extractor, funnel, filter paper, evaporating dish, spotter, silica gel G plate, chromatography cylinder, spray bottle, hair dryer.

[0061] (2) Control medicinal materials

[0062] Woody

[0063] (3) Reagents

[0064] Ethanol, methanol, ether, cyclohexane, ethyl acetate, dichloromethane, vanillin, sulfuric acid.

[0065] (4) Inspection method:

[0066] Selection of extraction solvent: use ethanol, methanol, ethyl acetate, ether and other solutions as extraction solvents;

[0067] Selection of extraction methods: ultrasonic and cold soaking respectively;

[0068] The choice of developer: use cyclohexane-dichloromethane-ethyl acetate (volume ratio 15:5:1) and cyclohexane-ethyl acetate (volume ratio 10:3) as the developer;

[0069] The above extraction solvents, extraction methods and developing agen...

experiment example 3

[0074] Experimental example 3: TLC detection of cassia seed

[0075] (1) Instrument

[0076] Mortar, electronic scale, measuring cylinder, flat bottom flask, condenser tube, rubber tube, water bath, ultrasonic extractor, funnel, filter paper, evaporating dish, separatory funnel, absorbent cotton, sample spotter, silica gel G plate, chromatography cylinder, ultraviolet spot analyzer.

[0077] (2) Control medicinal materials

[0078] Cassia

[0079] (3) Reagents

[0080] Ethanol, methanol, ethyl acetate, diethyl ether, chloroform, distilled water, anhydrous sodium sulfate, petroleum ether (60-90°C), ethyl formate, formic acid, 2.5mol / L sulfuric acid solution.

[0081] (4) Inspection method:

[0082] Selection of extraction solvent: use ethanol, methanol, ethyl acetate, ether, chloroform, 2.5mol / L sulfuric acid solution and other solutions as extraction solvents;

[0083] Selection of extraction methods: ultrasonic and heating reflux respectively;

[0084] The choice of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for detecting a traditional Tibetan medicine, namely a ten-flavour rankincense preparation. The method is used for detecting frankincense, elecampane and semen cassia in the ten-flavour rankincense preparation through a thin-layer chromatography and comprises the following steps of: (1), preparing a frankincense sample solution group, an elecampane sample solution group and a semen cassia sample solution group; and (2), respectively spotting the three groups of samples prepared in the step (1) on three silica gel G thin-layer plates, developing the samples through a developer, spraying a vanillic aldehyde sulfuric acid solution on the samples, air drying the samples till spots are developed clearly, and detecting the samples. The detection method disclosed by the invention carries out detection and research of frankincense, elecampane and semen cassia through the thin-layer chromatography; the improved thin-layer chromatography has the advantages of being good in repeatability and stability, simple and convenient for operation, high in precision, strong in specificity, clear in spot development, good in separation degree and the like; and the quality standard of the ten-flavour rankincense preparation can be effectively controlled by establishing a quality detection method which is reliable in method and strong in specificity, thus, the quality of the ten-flavour rankincense preparation is steady, controllable and safe.

Description

technical field [0001] The invention relates to a detection method of a pharmaceutical composition preparation, in particular to a detection method of a ten-flavor frankincense preparation, which belongs to the technical field of traditional Chinese medicine detection. Background technique [0002] Shiwei frankincense pills are Tibetan medicines, which are recorded in the Tibetan medicine sub-volume of "Drug Standards of the Ministry of Health of the People's Republic of China", and the standard number is WS3-BC-0211-95. [0003] Prescription: 100g frankincense, 150g myrobalan, 80g cassia, 100g myrobalan, 80g emblical, 120g emblical, 85g woody, 100g broad vine, 80g pashaga, 50g slag taming paste. [0004] Preparation method: the above ten flavors, except the slag taming paste, the rest are crushed into fine powder, sieved, mixed, and the slag taming paste is added with appropriate amount of water to form a pill, dried, and obtained. [0005] It is mainly used to treat redne...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
Inventor 才让卓玛张国霞陈丽娟
Owner TIBET QIZHENG TIBETAN MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products