Collagen purification method using CO2 supercritical fluidization method
A technology of supercritical fluid and collagen, which is applied in the preparation of animal glue or gelatin, the production of bulk chemicals, and the extraction of solvents from liquid solutions. Effect
Active Publication Date: 2005-01-26
JIANGYIN BENXIANG BIOTECHOLOGY
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Problems solved by technology
But so far there is no relevant use of CO 2 A report on the method of supercritical fluid purification of collagen
Method used
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Embodiment 1
[0035] Embodiment 1: 1000g of purified pigskin collagen
[0036] Reaction conditions: Temperature: 40°C Pressure: 16MPa
[0037] Time: 60min Speed: 55 rpm
Embodiment 2
[0038] Embodiment 2: 1000g of purified sheepskin collagen
[0039] Reaction conditions: Temperature: 36°C Pressure: 14MPa
[0040] Time: 80min Speed: 40 rpm
Embodiment 3
[0041] Embodiment 3: 1000g of purified bovine tendon collagen
[0042] Reaction conditions: Temperature: 45°C Pressure: 18MPa
[0043] Time: 90min Speed: 50 rpm
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The invention discloses a collagen purification method using CO2 supercritical fluidization method, wherein a depuration reactor is employed to substitute the abstraction apparatus, and CO2 hypercritical fluid is employed to purify the natural gum raw material having three-dimensional histological structure under two-dimensional treatment condition, thus effectively removing the grease, fabric mesenchyme and cells from the collagen.
Description
1. Technical field [0001] The present invention relates to a CO 2 The invention discloses a method for purifying collagen by supercritical fluid, which belongs to the technical field of supercritical fluid purification. 2. Background technology [0002] Supercritical fluid technology is a new chemical separation technology developed in recent decades. The process has low toxicity, less residue, and can be carried out at room temperature, so it is especially suitable for the separation and purification of unstable natural products and physiologically active substances, and is the most effective way to obtain high-quality products in the deep processing fields of food, spices, medicine and petroleum One of the means. [0003] Supercritical CO 2 Extraction technology is one of the most widely used technologies in the field of supercritical fluid technology, supercritical CO 2 Extraction technology is currently mainly used for extraction and purification in the spice industr...
Claims
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IPC IPC(8): B01D11/04C09H3/00
CPCY02P20/54
Inventor 廖隆理王坤余但卫华李志强曾睿陈敏
Owner JIANGYIN BENXIANG BIOTECHOLOGY
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