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A kind of preparation method of high-purity phycocyanin

A high-purity phycocyanin technology, applied in the field of preparation of high-purity phycocyanin, can solve the problems of phycocyanin decomposition, cumbersome operation, long cycle, etc., and achieve the effect of mild purification conditions, simple preparation process and low cost

Active Publication Date: 2020-12-22
JIANGXI DANXIA BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the purification method of phycocyanin has the disadvantages of high cost, cumbersome operation and long period. For example, CN104844707A discloses a method for extracting phycocyanin from spirulina. Repeated freezing and thawing in water, high-pressure homogenization, and then ultrasonic crushing; in the later stage of purification, centrifugation is required to extract the feed liquid; and high-pressure homogenization and ultrasonic crushing, especially high-pressure homogenization will generate high temperatures, which may easily cause algae Decomposition and destruction of blue pigment

Method used

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  • A kind of preparation method of high-purity phycocyanin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Put 300Kg of dry high-quality spirulina in a stainless steel stirring tank, add 2% sodium chloride solution according to the mass ratio of spirulina:salt solution=1:20, and stir at a low speed of 25°C for 7 hours. The dissolved feed liquid is transported to a plate and frame filter press through a pipeline, and the feed liquid is separated by the plate and frame filter press to obtain a crude extract of phycocyanin. Collect the crude phycocyanin extract, add food-grade powdered ammonium sulfate solid to it, until the saturation of ammonium sulfate in the mixed solution is 10%, then adjust the pH to 6.0, stir and mix at 10°C and let stand for 6h, remove The supernatant is diluted with pure water with a conductivity of 1 to 60 us, and then the impurity is removed by a 0.45 μm fine filter, and the filtered feed liquid is desalted and concentrated through an ultrafiltration membrane device with a molecular weight of 30,000 until When the conductivity of the permeate is belo...

Embodiment 2

[0033] Put 300Kg of dry high-quality spirulina in a stainless steel stirring tank, add 1% potassium chloride solution according to the mass ratio of spirulina:salt solution=1:35, and stir at a low speed of 10°C for 6 hours. The dissolved feed liquid is transported to a plate and frame filter press through a pipeline, and the feed liquid is separated by the plate and frame filter press to obtain a crude extract of phycocyanin. Collect the crude phycocyanin extract, add food-grade powdered ammonium sulfate solid to it, until the saturation of ammonium sulfate in the mixed solution is 12%, then adjust the pH to 6.2, stir and mix at 20°C and let it stand for 6h, remove The supernatant is diluted with pure water with a conductivity of 1 to 60us, and then the impurity is removed by a 0.3μm fine filter, and the filtered feed liquid is desalted and concentrated through an ultrafiltration membrane device with a molecular weight of 20,000 until When the conductivity of the liquid is bel...

Embodiment 3

[0035] Put 300Kg of dry high-quality spirulina in a stainless steel mixing tank, add 2% sodium dihydrogen phosphate solution according to the mass ratio of spirulina:salt solution = 1:50, and stir at a low speed of 30°C for 8 hours. The dissolved feed liquid is transported to a plate and frame filter press through a pipeline, and the feed liquid is separated by the plate and frame filter press to obtain a crude extract of phycocyanin. Collect the crude extract of phycocyanin, add food-grade powdered ammonium sulfate solid to it, until the saturation of ammonium sulfate in the mixed solution is 15%, then adjust the pH to 6.5, stir and mix at 30°C and let stand for 6h, remove The supernatant is diluted with pure water with a conductivity of 1 to 60 us, and then the impurity is removed by a 0.22 μm fine filter, and the filtered feed liquid is desalted and concentrated through an ultrafiltration membrane device with a molecular weight of 10,000 until it is transparent When the con...

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Abstract

The invention discloses a preparation method of high-purity spirulina blue. The preparation method is characterized by adopting spirulina powder as a raw material, and performing continuous productionincluding extraction, filtration, salting out, desalination concentration, micro-encapsulation, spray drying and mixing to obtain spirulina blue, which is blue powder. Phycocyanin is dark blue powder, is separated from spirulina, and looks beautiful. The main component of spirulina blue is phycocyanin, which belongs to porphyrin cyanophycin and is one of infrequent chromoproteins in the nature. The phycocyanin has bright color, is protein itself with abundant nutrients, has complete amino acid composition, and is high in amino acid content. The preparation method is short in production periodand doesn't cause secondary pollution. The obtained spirulina blue is high in purity, contains 71.3% of phycocyanin, has the purity A620 / A280 reach 3.6, and can meet the requirement of food and cosmetic industry.

Description

technical field [0001] The invention relates to a preparation method of high-purity phycocyanin, which belongs to the field of deep processing of agricultural products. Background technique [0002] Spirulina, also known as Arthrospira or cyanobacteria, belongs to the phylum Cyanophyta, mainly including Spirulina maxima, Spirulina pureatops and Spirulina spirulina. Spirulina is extremely rich in nutrients. It contains a lot of protein, vitamins and minerals. It can be used as a high-protein, low-fat, low-cholesterol, low-calorie health food. It is recommended by the Food and Agriculture Organization of the United Nations as "the most ideal human tomorrow food". As spirulina is increasingly being developed as high-protein functional food, health products, etc., the development and utilization of spirulina will also have a broader prospect. [0003] The phycocyanin in spirulina is an important light-harvesting pigment protein, and it has the functions of anti-cancer, promoti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/795C07K1/14
CPCC07K14/795
Inventor 张军兵熊勇李冬梅罗忠国刘江波吴升王军潮张水军
Owner JIANGXI DANXIA BIOTECHNOLOGY CO LTD
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