Method for separating and purifying pig ganglioside

A technology for the separation and purification of gangliosides, which is applied in the field of high-efficiency separation and purification of porcine gangliosides, can solve the problems of low yields, and achieve the effects of high yields, high recovery rates, and stable yields
CN109705176AInactive Publication Date: 2019-05-03SUZHOU NANOMICRO TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU NANOMICRO TECH CO LTD
Publication Date
2019-05-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for efficiently separating and purifying porcine ganglioside, which comprises the steps of: dissolving and filtering a crude extract of the pig ganglioside; applying afiltered solution to a chromatography column filled with a homogenous normal-phase silica gel for chromatography; using ultrapure water and an organic solvent as a flow phase for elution of a target product; collecting the solution of the target peak in stages, and collecting the component liquid meeting the requirements. The method is applied to the deep purifying of the pig ganglioside, and onlyneeds one-step chromatography to satisfy the requirement that the pig ganglioside purity is greater than 99% and the yield is greater than 93.0%. The purification yield is high and stable, and the separation method of the invention is simple and convenient, can be used for large-scale production, and the production cost is greatly reduced.
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Description

technical field

[0001] The invention relates to the field of medicine purification, in particular to a method for efficiently separating and purifying porcine gangliosides. Background technique

[0002] Porcine ganglioside (ganglioside) exists more in the cell membrane, and its relationship with membrane function has attracted attention. The exotoxins of bacteria tetanus and cholera can specifically bind to specific porcine ganglioside molecules. Soluble in water and organic solvents. In water, it exists in the state of micelles aggregated by millions of molecules. Porcine ganglioside has a significant role in promoting nerve regeneration. Nerve growth factor (NGF) and porcine ganglioside (GM) are essential substances for the regeneration and development of brain nerves. GM mediates NGF to enhance its activity dozens of times and form new Rich neural network, repair and promote the development of cranial nerves again.

[0003] The structural formula is as follows: [00...

Claims

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