Method for extracting epigoitrin from isatis root with high extraction ratio

Abstract

The invention relates to a method for extracting epigoitrin from isatis root with high extraction ratio, comprising the following steps: 1. isatis root medicinal material is crushed into coarse powder, 10-25 times of water or low-concentration ethanol is added in the coarse powder for being extracted at the temperature of 20-70 DEG C; 2. filtrate is decompressed and concentrated at the temperature of 40-70 DEG C for obtaining epigoitrin constituent; 3. concentrated solution passes through processed macroporous resin, and is washed by 5-12 times of column volume of water, and is washed by 2-5 times of column volume of water, and is washed by 30 percent to 50 percent of ethanol by mass concentration and then is collected; 4. ethanol eluent is decompressed and concentrated at the temperature of 40-70 DEG C, thereby obtaining epigoitrin macroporous resin separated constituent; 5. the epigoitrin macroporous resin separated constituent is separated by column chromatography or high-efficiency industrial chromatographic column, thereby preparing into epigoitrin monomer. Compared with the prior art, the extraction efficiency of the epigoitrin is 2.4 times higher than that of the prior art; in addition, the period is short, the impurities are less, the post-processing is convenient, the raw material can be fully utilized, the cost can be shortened and economic benefit is improved.

Description

Technical field [0001] The invention relates to a process for extracting effective components of traditional Chinese medicine, in particular to a method for extracting Yichun from Isatis indigotica with a high extraction rate. Background technique [0002] Isatis indigotica is a traditional Chinese anti-viral medicine. The first part of "The Pharmacopoeia of the People's Republic of China" (2005 edition) is included as the dried root of the cruciferous plant Isatisindigotica Fort. It was originally published in "Shen Nong's Materia Medica". The effect of blood to relieve the pharynx is often used clinically for viral and bacterial infections. Table Gao Yichun, whose English name is Epigoitrin, is a kind of sulfur-containing alkaloids, and its structural formula is as follows: [0003] [0004] Literature reports report that Yichun is the main component of Isatis indigotica (see: ① Liu Haili, Wu Lijun, Li Hua, et al. Studies on the chemical constituents of Isatis indigotica[J]. ...

AI Technical Summary

Problems solved by technology

At present, literatures and related patents report that the methods for extracting Yichun include 6-12 times the amount of 30-95% ethanol for reflux extraction, and 8-12 times the amount of water decoction for extraction, (see: ① Huang Fang, Xiong Yating, Xu Lihua , et al. Tables of antiviral components in different extracts of Radix Isatidis reported the pharmacokinetics of Yichun in rats [J]. Journal of China Pharmaceutical University, 2006, 37(6): 519-522. ② Xu Lihua. From Radix Radix A method for extracting Biaoyichun and its use [P]. Chinese patent: CN1666984, 2005-09-14. ③Liang Xinmiao, Feng Jiatao, Xu Qing, etc. A separation and preparation method of Biaoyichun [P] ]. Chinese patent: CN101289429A, 2008-10-22.), these methods have the disadvantages of low extraction rate, long time consumption, many extracted impurities, cumbersome post-processing, etc.

Images