Method for extracting epigoitrin from isatis root with high extraction ratio

A technology that reports Yichun and Isatis root, applied in organic chemistry, antiviral agents, etc., can solve the problems of cumbersome post-processing, low extraction rate, and many impurities, and achieve the effect of convenient subsequent purification, high extraction efficiency, and simple process.

A technology that reports Yichun and Isatis root, applied in organic chemistry, antiviral agents, etc., can solve the problems of cumbersome post-processing, low extraction rate, and many impurities, and achieve the effect of convenient subsequent purification, high extraction efficiency, and simple process.

CN101602740AInactive Publication Date: 2009-12-16贾晓斌
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  • Method for extracting epigoitrin from isatis root with high extraction ratio
  • Method for extracting epigoitrin from isatis root with high extraction ratio
  • Method for extracting epigoitrin from isatis root with high extraction ratio

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Example 1: Weigh 50g of Radix isatidis crude powder (passed through a 20-mesh sieve), add 20 times the amount of water, and microwave-assisted extraction at 60°C for 10 minutes, filter, and dilute the filtrate as the test sample. The HPLC test table tells Yichun’s Concentration, calculated to extract 118.49mg of Yichun, and the extraction rate calculated according to the following formula is 92.34%.

[0053]

Embodiment 2

[0054] Example 2: Weigh 200g of Radix Isatidis crude powder (passed through a 20-mesh sieve), add 20 times the amount of water to 60°C microwave assisted extraction for 10 minutes, filter, and dilute the filtrate as the test sample. The HPLC test table tells Yichun’s Concentration, calculated to extract 478.12mg of Yichun, the extraction rate calculated according to the following formula is 93.13%.

Embodiment 3

[0055] Example 3: Weigh 500g of Radix Isatidis crude powder (passed through a 20-mesh sieve), add 20 times the amount of water to 60°C microwave assisted extraction for 10 minutes, filter, and dilute the filtrate as the test sample. The HPLC test table tells Yichun’s Concentration, calculated to extract the amount of Yichun Yichun is 1196.22mg, the extraction rate calculated according to the following formula is 93.20%.

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Abstract

The invention relates to a method for extracting epigoitrin from isatis root with high extraction ratio, comprising the following steps: 1. isatis root medicinal material is crushed into coarse powder, 10-25 times of water or low-concentration ethanol is added in the coarse powder for being extracted at the temperature of 20-70 DEG C; 2. filtrate is decompressed and concentrated at the temperature of 40-70 DEG C for obtaining epigoitrin constituent; 3. concentrated solution passes through processed macroporous resin, and is washed by 5-12 times of column volume of water, and is washed by 2-5 times of column volume of water, and is washed by 30 percent to 50 percent of ethanol by mass concentration and then is collected; 4. ethanol eluent is decompressed and concentrated at the temperature of 40-70 DEG C, thereby obtaining epigoitrin macroporous resin separated constituent; 5. the epigoitrin macroporous resin separated constituent is separated by column chromatography or high-efficiency industrial chromatographic column, thereby preparing into epigoitrin monomer. Compared with the prior art, the extraction efficiency of the epigoitrin is 2.4 times higher than that of the prior art; in addition, the period is short, the impurities are less, the post-processing is convenient, the raw material can be fully utilized, the cost can be shortened and economic benefit is improved.

Description

Technical field [0001] The invention relates to a process for extracting effective components of traditional Chinese medicine, in particular to a method for extracting Yichun from Isatis indigotica with a high extraction rate. Background technique [0002] Isatis indigotica is a traditional Chinese anti-viral medicine. The first part of "The Pharmacopoeia of the People's Republic of China" (2005 edition) is included as the dried root of the cruciferous plant Isatisindigotica Fort. It was originally published in "Shen Nong's Materia Medica". The effect of blood to relieve the pharynx is often used clinically for viral and bacterial infections. Table Gao Yichun, whose English name is Epigoitrin, is a kind of sulfur-containing alkaloids, and its structural formula is as follows: [0003] [0004] Literature reports report that Yichun is the main component of Isatis indigotica (see: ① Liu Haili, Wu Lijun, Li Hua, et al. Studies on the chemical constituents of Isatis indigotica[J]. ...

Claims

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Application Information

Patent Timeline
16 Dec 2009
Publication
CN101602740A
IPC
C07D263/16; A61P31/12
Inventors
贾晓斌; 陈彦