52 results about "Isatis indigotica" patented technology

The invention relates to a cultivation method of an overexpressed EVM0003075 gene isatis tinctoria plant, and belongs to the technical field of plant cultivation. The method comprises the following steps: firstly, transforming an agrobacterium strain through a constructed agrobacterium transformation vector for expressing the isatis tinctoria EVM0003075 gene, then transforming an explant of the isatis tinctoria by using a screened recombinant agrobacterium strain, regenerating a seedling of the isatis tinctoria, and screening and culturing the seedling of the isatis tinctoria with stable overexpression of the EVM0003075 gene, so as to obtain an isatis tinctoria plant with increased isatin content in the isatis tinctoria. The expression level of the EVM0003075 gene of the transgenic isatis tinctoria is improved to 5-9 times, the content of the isatis tinctoria is remarkably improved, and the character that the accumulation period of the isatis tinctoria is short is shown. By adopting the method disclosed by the invention, the content of the isatin in the isatis tinctoria can be increased to 10-66 times. The specific information content of the patent of the method for improving the content of the isatin in the isatis tinctoria through overexpression of the EVM0003075 gene is shown in the specification.

The present invention provides a high-protein bio-fermented aquatic feed and a preparation method thereof. The aquatic feed comprises the following raw materials in parts by weight: 20-30 parts of imported fish meal, 10-15 parts of compound minerals, 5-10 parts of bioactive peptides, 5-10 parts of lysine, 5-10 parts of compound trace elements, 3-8 parts of compound vitamins, 3-6 parts of herbal extracts, 2-5 parts of compound probiotics, 2-4 parts of immune polysaccharides, and 1-3 parts of compound enzymes; the herbal extracts are prepared by fermenting astragalus membranaceus, isatis root, liquorice root, calotropis gigantea, ventilago leiocarpa benth, clove, rhizoma chuanxiong, and galangal officinale. By organically integrating imported fish meal, astaxanthin-peptide chelate, herbal extracts, compound immune polysaccharides, compound probiotics and various nutrients, the present invention realizes the synergistic effect of "promoting feeding - enhancing digestion - strengthening liver and gall - enhancing immunity - resisting stress - inhibiting bacteria - stabilizing water quality", significantly improves the growth rate, survival rate and comprehensive breeding benefit of crustacean aquatic animals, and has good economic value and application prospect.

This invention discloses a method for preparing a heat-clearing and detoxifying oral liquid. Honeysuckle and Scutellaria baicalensis are pulverized and sieved, then enzymatically hydrolyzed with water, ultrasonically extracted, and filtered to obtain extract A. Scrophularia ningpoensis, Rehmannia glutinosa, Forsythia suspensa, Gardenia jasminoides, Viola yedoensis, Gentiana scabra, Isatis indigotica, Anemarrhena asphodeloides, and Ophiopogon japonicus are warm-soaked in water and flash-extracted, then filtered to obtain extract B. Gypsum is crushed, decocted with water, and filtered to obtain extract C. The extracts are combined, concentrated, and sequentially clarified using a flocculant, followed by inorganic ceramic membrane microfiltration and organic ultrafiltration. The clarified liquid is then concentrated, flavoring agents and preservatives are added, the pH is adjusted, the volume is adjusted, filtered, filled, and sterilized to obtain the finished product. This invention utilizes a composite clarification process—low-temperature ultrasonic-enzymatic extraction of honeysuckle and Scutellaria baicalensis, clarification using a flocculant, inorganic ceramic membrane microfiltration, and organic ultrafiltration—to improve the extraction rate of effective components, effectively remove large molecular impurities such as tannins, proteins, and pectin, and significantly improve the clarity and stability of the product.

This invention provides a drug for antagonizing bacterial endotoxins, its preparation method, and its application, belonging to the biomedical field. The composition consists of 25-55 parts of dried powdered traditional Chinese medicine extract, 25-70 parts of mixed essential oil powder, and 40-90 parts of lentinan powder. The traditional Chinese medicine extract is prepared by decoction of Scutellaria baicalensis, Lonicera japonica, Forsythia suspensa, and Isatis indigotica under reduced pressure at low temperature and spray drying. The mixed essential oil powder is prepared by encapsulating peppermint oil and eucalyptus oil with gelatin and hydroxypropyl cellulose and then spray drying under high pressure. This invention, through specific formulation and processing, significantly enhances the synergistic antagonistic effect of each component against endotoxins. Experiments show that this composition exhibits antagonistic activity far superior to ibuprofen in vitro; in vivo, it significantly reduces the endotoxin content in the plasma of 817 broiler chickens, rapidly alleviates LPS-induced fever and diarrhea, and improves decreased growth performance. Therefore, this invention is of great significance for reducing antibiotic dependence and improving the efficiency of livestock and poultry farming.

The invention discloses a composition containing an astragalus extract as well as a preparation method and application of the composition. The composition comprises a carrier layer, a core functional layer, an assistant functional layer, a functional powder layer and a probiotic layer from inside to outside. According to the invention, monarch and ministerial drugs (astragalus membranaceus, codonopsis pilosula, bighead atractylodes rhizome and poria cocos) are taken as a core layer through a fluidized bed, adjuvant and conductant drugs (isatis root, dandelion and platycodon grandiflorum) are taken as a secondary layer, sequence batch layering is realized, and directional release can be realized.

1. Name of the product in this design: Packaging Box (Isatis Root Tea). 2. Purpose of this design: For packaging pharmaceuticals. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: a 3D model.

1. Name of the product in this design: Plush Toy (Longjiuwei's Isatis Root). 2. Purpose of this design: plush toy. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

This utility model provides an integrated water and fertilizer irrigation device for Isatis indigotica. The device includes: a base plate; a water and fertilizer mixing tank and a water storage tank, both fixedly mounted on the base plate; a fertilizer storage tank, fixedly mounted on the water and fertilizer mixing tank, with its bottom connected to the mixing tank, and an installation box on the storage tank containing a drying plate; magnetic sheets on the inner wall of the installation box and the drying plate; a water supply mechanism located on the mixing tank for providing water for the water and fertilizer mixture; and an irrigation mechanism located on the mixing tank for irrigating the Isatis indigotica with water and fertilizer. This integrated water and fertilizer irrigation device for Isatis indigotica allows for better application of water and fertilizer to the plant, improving water and fertilizer utilization.

The invention provides Qingkailing dropping pills and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine extraction and preparations, the Qingkailing dropping pills comprise the following raw materials: cholic acid, nacre mother of pearl, hyodeoxycholic acid, cape jasmine, cornu bubali, isatis root, baicalin and honeysuckle; the preparation method comprises the following steps: providing pill paste powder, wherein the pill paste powder is prepared by sequentially drying mixed thick paste under reduced pressure and crushing; the mixed thick paste comprises the following raw materials: a honeysuckle extract, an isatis root-cape jasmine extract, a buffalo horn-nacre mother-of-pearl extract and a cholic acid-baicalin concentrated solution; providing a matrix, and melting and mixing the matrix with the pill paste powder to prepare a molten liquid; and providing a condensing agent, dropwise adding the molten liquid into the condensing agent, and carrying out shrinkage molding, so as to obtain the finished product dropping pill, namely the Qingkailing dropping pill. According to the preparation method, the melting and mixing efficiency and effect of the pill paste powder and the matrix are improved, the texture of molten liquid is more uniform, the content distribution of all effective components is more uniform, the medicine content uniformity in the finished dropping pill is improved, and the obtained finished dropping pill is high in stability, round in appearance and good in uniformity.

The invention discloses a crocodile functional compound feed and a preparation method thereof, and the crocodile functional compound feed comprises the following raw materials: a compound animal protein source, a compound vegetable protein and energy source, a core functional additive, a compound grease source, a compound premix and a phosphorus calcium source, the functional additive comprises astragalus membranaceus, isatis root, radix bupleuri, liquorice, oriental wormwood, cowherb seed, astaxanthin, nano-selenium and zinc methionine, the compound premix comprises vitamin and mineral components, and the bait coefficient of the compound feed is lower than 1.5. According to the feed, chilled fresh fish can be replaced with the efficient replacement ratio of 1: 5, the breakthrough breeding effects that the survival rate of crocodiles is increased by 30%, the growth rate is increased by 20%, the laying rate is increased by 40%, and the bait coefficient is reduced to 1.3 or below are achieved, and the economic benefits are extremely remarkable.

This application belongs to the field of seed detection and analysis technology, specifically relating to a method for constructing a characteristic chromatogram of Isatis indigotica seeds and a method for detecting Isatis indigotica seeds. The construction method includes the following steps: providing a reference solution; preparing a test solution using Isatis indigotica seeds; detecting the reference solution and the test solution using ultra-high performance liquid chromatography (UHPLC); importing the chromatograms obtained from detecting the reference solution and the test solution into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system to establish a characteristic chromatogram of Isatis indigotica seeds; the reference solution contains alkaloids as a reference standard; the UHPLC method meets the following conditions: the stationary phase includes a C18 column; the mobile phase includes mobile phase A and mobile phase B, mobile phase A including methanol and / or mobile phase B including water; and a gradient elution program is used. This application uses UHPLC to construct a characteristic chromatogram reflecting the secondary metabolites of Isatis indigotica seeds, providing a novel reference for evaluating the quality of Isatis indigotica seeds.

The application discloses a traditional Chinese medicine exosome compound with anti-HPV and application thereof, and relates to the technical field of biotechnology.The compound comprises functional components secreted by a human cell co-culture system and a traditional Chinese medicine source extract, and the traditional Chinese medicine source extract is derived from a combined traditional Chinese medicine extract of Astragalus membranaceus, Isatis indigotica Fort., Honeysuckle and Sophora flavescens Ait., and relates to the technical field of biotechnology.The traditional Chinese medicine exosome carries natural active ingredients, and a multi-target anti-virus network is formed by beta-defensin-2 and interferon-gamma generated by co-culture with human cells.Compared with single traditional Chinese medicine extract or chemically synthesized nanoparticles, the HPV inhibition rate is increased by more than 170%, and the direct cytotoxicity of traditional Chinese medicine is reduced through an exosome delivery system, and the IC 50 >200ug / mL, which is significantly lower than that of a water decoction, and mucous membrane irritation experiments show no damage.

This invention discloses the application of Isatis root polysaccharide in improving egg quality or gut health in poultry, belonging to the field of poultry farming technology. This invention provides the application of Isatis root polysaccharide in improving egg quality or gut health in poultry. Applying Isatis root polysaccharide to poultry farming can effectively improve egg quality and gut health. Furthermore, the preparation process of the Isatis root polysaccharide of this invention is simple, as it can be directly obtained from the pharmaceutical process of Isatis root granules, resulting in low cost. A small amount of the polysaccharide can achieve good results. The Isatis root polysaccharide of this invention can reduce poultry farming costs, alleviate the economic burden on farmers, and is suitable for widespread promotion.

This invention relates to the field of feed additive technology, specifically to a compound plant-seaweed feed additive for reducing methane emissions in ruminants and its preparation method. It comprises the following raw materials in parts by weight: 40-60 parts sorghum husk powder, 30-50 parts purple sweet potato powder, 0.5-5 parts functional additives, 5-10 parts red algae, 5-10 parts brown algae, 5-12 parts *Lactobacillus plantarum*, 3-8 parts chitosan, 2-6 parts essential oil, 2-5 parts 3-nitrooxypropanol, 10-15 parts *Scutellaria baicalensis*, 8-12 parts *Codonopsis pilosula*, 3-8 parts *Isatis indigotica*, and 5-18 parts modified zeolite. Through the regulation of *Lactobacillus plantarum* and chitosan, the energy originally used to generate methane is converted into propionic acid. Propionic acid is a major gluconeogenic precursor in ruminants, which is directly converted into glucose for milk production or muscle growth.

This invention discloses a precision seeding device based on granulated Isatis indigotica seeds, comprising a base, a support frame fixedly connected to the base, a storage trough fixedly connected to the support frame, a feeding pipe at the bottom of the storage trough, the feeding pipe being fixedly connected to a fixed box, a second motor on the side of the fixed box, the output end of the second motor being fixedly connected to a round block, and the round block being rotatably connected to the fixed box. In this invention, the device integrates precision seeding, grooving, and soil covering into a single operation, eliminating the need for manual completion of seeding and soil covering processes, significantly reducing labor intensity. Simultaneously, precision control is achieved by adjusting the grooving and seed storage space using sliding blocks, reducing seed waste and avoiding the inaccurate seed quantity in traditional hill-planting techniques, thus ensuring fewer thinning steps. Furthermore, the integrated operation mode avoids the inefficiency of scattered manual operations, ensuring seeding progress and quality, and effectively alleviating planting cost pressures.

The present application relates to the technical field of drug efficacy detection, and particularly relates to a test method of Suguanning capsules. The present application adds the detection standards of Isatis indigotica Fort, Siphonostegia chinensis Maxim, Lonicera japonica and paracetamol and Bupleurum chinense to the original standard of Suguanning capsules, carries out thin layer chromatography on Isatis indigotica Fort, Siphonostegia chinensis Maxim and Lonicera japonica, and carries out the detection of specificity, linear relationship, precision, stability, reproducibility and sample recovery rate on paracetamol and Bupleurum chinense. The thin layer chromatography reproducibility and specificity of Bupleurum chinense and Lonicera japonica are good, and the negative has no interference, so the quality standard text is included. The thin layer chromatography specificity of Siphonostegia chinensis Maxim and Isatis indigotica Fort is poor, so the quality standard text is not included, and the detection standard of Suguanning capsules is improved.

The invention discloses a traditional Chinese medicine active extract for preventing and treating asparagus stem blight and a preparation method thereof, and belongs to the technical field of crop planting, the traditional Chinese medicine active extract is prepared from the following raw materials in parts by weight: 20-30 parts of sweet wormwood, 15-20 parts of radix scutellariae, 15-25 parts of rhizoma smilacis glabrae, 8-12 parts of radix sophorae flavescentis, 9-17 parts of radix stephaniae tetrandrae, 2-5 parts of perilla frutescens, 10-15 parts of folium isatidis, 8-10 parts of cyrtomium fortunei and 3-5 parts of indigo naturalis. The preparation method comprises the following steps: crushing the raw materials, uniformly mixing, adding an ethanol solution, carrying out ultrasonic extraction, carrying out aerobic fermentation on the obtained extracting solution, finally carrying out evaporation concentration on the obtained fermentation solution, and carrying out spray drying to obtain the traditional Chinese medicine active extract. According to the invention, various Chinese herbal medicine raw materials are carefully selected, the ratio is optimized, and the processes of crushing, mixing, ethanol ultrasonic extraction, aerobic fermentation, spray drying and the like are combined, so that active components are effectively extracted. An efficient and natural solution is provided for preventing and treating the asparagus stem blight.

The invention discloses a traditional Chinese medicine composition for treating precancerous lesions of pulmonary nodules and a preparation method of the traditional Chinese medicine composition. The composition is prepared from 30 parts of coix seeds, 15 parts of astragalus membranaceus, 30 parts of pyrrosia lingua, 10 parts of raw rhizoma atractylodis macrocephalae, 10 parts of fried hawthorn, 10 parts of folium isatidis, 30 parts of oysters, 15 parts of caulis spatholobi, 30 parts of oldenlandia diffusa, 10 parts of fructus lycii, 10 parts of glossy privet fruits, 15 parts of radix glehniae, 3 parts of gecko, 6 parts of liquorice, 15 parts of codonopsis pilosula, 6 parts of cassia twigs, 30 parts of salvia chinensis, 15 parts of Chinese waxgourd seeds, 10 parts of verbena and 10 parts of asparagus cochinchinensis. During preparation, the medicinal materials are selected and crushed, and refined honey is added to prepare pills. Clinical research shows that after the preparation is taken for 6 months, the maximum diameter of pulmonary nodules of a patient is remarkably reduced, the malignant probability of mei-ovum is reduced from 16.60% to 8.33%, the integral of traditional Chinese medicine syndromes is reduced from 10.91 to 6.91, and the serum CEA is reduced from 2.0 ng / mL to 1.51 ng / mL. The traditional Chinese medicine composition has the effects of tonifying qi and yin, reducing phlegm, removing toxins and eliminating diseases aiming at the lung nodule precancerous lesion with the syndrome of vital qi deficiency and phlegm stagnation, and is definite in curative effect and high in safety.

The invention discloses an isatis root decoction piece preparation device, and belongs to the technical field of isatis root processing, the isatis root decoction piece preparation device comprises a supporting bottom plate, a drying mechanism is arranged above the supporting bottom plate, and a plurality of limiting supporting legs for limiting and supporting the drying mechanism are further arranged above the supporting bottom plate; a plurality of elastic supporting pieces for limiting and supporting the limiting supporting legs are arranged at the two ends of the upper surface of the supporting bottom plate, an air supply mechanism for vibrating and supplying air to the drying mechanism is arranged on the upper surface of the supporting bottom plate, and the air supply mechanism is fixedly connected with the supporting bottom plate; through mutual cooperation of multiple structures, isatis root slices are stored in a scattered mode so as to reduce the stacking effect of the isatis root slices, the isatis root slices are driven to vertically move in a reciprocating mode so as to ensure that each isatis root slice can make full contact with air, and the isatis root slices can be rapidly and evenly dried in cooperation with all-directional spraying of hot air.

The invention relates to the technical field of traditional Chinese medicines, and particularly provides a traditional Chinese medicine composition for treating osteoporosis and a preparation method and application thereof. The traditional Chinese medicine composition for treating osteoporosis is prepared from fructus lycii, fructus schizandrae, fructus rubi, semen cuscutae, semen plantaginis, fructus ligustri lucidi, semen allii tuberosi, radix angelicae sinensis, rhizoma chuanxiong, herba epimedii, radix dipsaci and radix morindae officinalis. The traditional Chinese medicine composition can be preferably decocted into a decoction, has an action mechanism verified by research in the aspect of treating osteoporosis, and inhibits the function of osteoclasts by inhibiting the expression of RXR alpha and RXR beta, thereby reducing the number of osteoclasts and achieving the effect of treating osteoporosis.

The invention relates to a molecular marker combination for identifying isatis tinctoria brassica and application thereof.The molecular marker combination is developed on the basis of a newly-assembled isatis tinctoria T2T reference genome and colinear analysis of isatis tinctoria g chromosome and brassica napus A and C subgenomes, and key areas (short arms, long arms and centromere) of the isatis tinctoria g chromosome can be covered; the method has the advantages of high specificity, comprehensive coverage, simplicity and rapidness in operation, low cost and the like, can rapidly and accurately identify whether the isatis tinctoria g chromosome exists in the isatis tinctoria brassica plant or not and the structural integrity of the isatis tinctoria g chromosome in the seedling stage, and effectively solves the problems that an existing identification method is complex in operation, poor in timeliness and limited in accuracy. Therefore, the production purity and efficiency of the isatis root oil flowering Chinese cabbage are remarkably improved and guaranteed, and the method can be used for identifying authenticity of products and has wide application prospects and popularization value.

The invention belongs to the technical field of textile, and discloses a carbon quantum dot spunlaced fabric, application thereof in sanitary napkins and a preparation method of the carbon quantum dot spunlaced fabric. The core of the formula is as follows: a functional carbon quantum dot carbon source is medicinal and edible powder formed by mixing scutellaria baicalensis, isatis root and folium artemisiae argyi according to a ratio of 2: 1: 1, so that broad-spectrum antibacterial and anti-inflammatory effects are realized; the spunlace base material is composed of bamboo fibers, hollow polyester fibers, ES fibers, high-strength polyester staple fibers and chitin fibers according to the proportion of 5: 2: 5: 3: 4, and the fibers cooperate to guarantee breathability, elasticity, mechanical properties and permeability; auxiliary reagents are citric acid, PVP and deionized water, no toxic solvent is used in the whole process, and the sanitary towel is green and environmentally friendly and meets the requirements of sanitary towels.

The application belongs to the technical field of textiles, and relates to an indigo pre-reducing agent, an indigo reducing paste and application thereof. The indigo pre-reducing agent is prepared from the following raw materials: isatis indigotica leaf, indigo fermentation liquor, glutinous rice wine and water; the indigo reducing paste is prepared from the following raw materials: the indigo pre-reducing agent, an alkaline compound, a sugar-based reducing agent, water, a penetrating agent and a natural thickening agent. The plant indigo reducing paste provided by the application can be used for printing and dyeing at room temperature, and the process of fixing color by high-temperature steaming in the printing process is omitted, so that energy consumption is saved. Meanwhile, the sugar-based reducing agent is used to replace the chemical reducing agent, so that damage of the traditional chemical reducing agent to protein fibers is avoided, and the printing paste can be applied to various fiber fabrics. The indigo pre-reducing agent and the plant indigo reducing paste provided by the application have wide application prospects in green textiles.

The invention relates to the technical field of traditional Chinese medicine fermentation, in particular to a preparation method of compound traditional Chinese medicine probiotic fermentation liquor for treating chicken colibacillosis, the fermentation liquor and application. Wherein the traditional Chinese medicine composition comprises the following raw materials: coptis chinensis, golden cypress, scutellaria baicalensis, isatis root, rheum officinale and liquorice; s2, preparing a strain culture medium; s3, adding the compound extracting solution into a strain culture medium to prepare a fermentation culture medium; s4, inoculating probiotics to the fermentation culture medium for fermentation; a traditional medicinal and edible traditional Chinese medicine compound is fermented by using bacillus subtilis, and the traditional Chinese medicine compound is biologically converted, so that the strain activity and the effectiveness of the medicinal and edible traditional Chinese medicine compound can be remarkably enhanced, and the content of active substances in a traditional Chinese medicine compound extracting solution is increased; meanwhile, the compounds in the compound can provide nutrient substances for the growth of the bacillus subtilis and promote the growth of the bacillus subtilis, so that the effects of the traditional Chinese medicines and the probiotics are exerted to the greatest extent.

The invention discloses a device and a method for sowing under-forest radix isatidis in saline-alkali soil in cooperation with soil improvement operation, relates to the technical field of agricultural planting instruments, and aims at a planting mode of single-layer ditching sowing and direct spraying of a modifier in the prior art. The dynamic adjustment of the application amount of the modifier and the double-layer ditching cooperative sowing treatment cannot be carried out according to the soil salinity. A layered structure of applying a modifier on an upper layer and sowing seeds on a lower layer is adopted, so that accurate space matching of the modifier and the seeds is realized, and the problem of mixed laying or distribution disjunction of the modifier and the seeds in existing equipment is solved; meanwhile, a dynamic soil detection and precise quantity control system is integrated, so that the real-time dynamic adjustment of the application amount of the modifier and the layered precise application of the modifier are realized, the salt in a seed growth area is effectively neutralized, the waste of the modifier is avoided, and the soil improvement efficiency and the seeding emergence rate are remarkably improved; the technical problems of multi-process separation, low operation efficiency and high salt return risk in the prior art are solved.

The invention relates to an optimization method for a pressing process of folium isatidis mouth decoction pieces, which can overcome the defects of leaf medicinal materials in production, transportation and application, and can be used for preparing the folium isatidis mouth decoction pieces by taking a single-factor test as a basis, combining a Box-Behnken design-response surface method and taking indirubin, tryptanthrin, isovitexin and water-soluble extracts as comprehensive evaluation indexes. Four factors including pressing time, pressure, baking temperature and baking time are investigated, an analytic hierarchy process and an entropy weight method are utilized to calculate weight coefficients and comprehensive scores of all indexes to determine the optimal pressing process of the folium isatidis mouth decoction pieces, and the optimal pressing process is close to a predicted value of a result obtained by actual operation, which shows that the process optimization method is good in predictability and high in practicability. The technology is stable and feasible, actual production can be directly guided, the problems of fuzzy technological parameters, large decoction piece quality fluctuation and the like in production are solved, key technical reference can be provided for standardized formulation of the folium isatidis mouth decoction piece pressing technology, and the practical application and popularization value is achieved.

This invention belongs to the field of biomedical technology and discloses a polysaccharide from Isatis indigotica root, its preparation method, and its applications. It comprises the following components in molar percentage: arabinose 26.17%, galactose 23.61%, rhamnose 23.01%, glucose 13.05%, xylose 6.69%, mannose 6.06%, and fucose 1.40%-1.41%. This polysaccharide is a homogeneous single component with a weight-average molecular weight of 38.114 kDa and a polydispersity index of 1.253. This invention employs a purification process combining water extraction and alcohol precipitation with enzymatic protein desorption, ion exchange, and gel column chromatography, which is stable and controllable, suitable for large-scale production. Experiments have confirmed that this polysaccharide can significantly enhance the activity of antioxidant enzymes such as superoxide dismutase at non-toxic concentrations, reduce reactive oxygen species levels, and alleviate oxidative stress damage in cells and zebrafish. It can be used to prepare antioxidant compositions for pharmaceuticals, health products, and functional foods.

The invention belongs to the technical field of seed detection and analysis, and particularly relates to a construction method of a specific chromatogram of isatis tinctoria seeds and a detection method of the isatis tinctoria seeds. The construction method comprises the following steps: providing a reference substance solution, preparing a test solution by using isatis tinctoria seeds, detecting the reference substance solution and the test solution by adopting ultra-high performance liquid chromatography, and introducing a chromatogram obtained by detecting the reference substance solution and a chromatogram obtained by detecting the test solution into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system. Formulating a characteristic spectrum of the isatis tinctoria seeds; a reference substance in the reference substance solution comprises alkaloid; the ultra-high performance liquid chromatography meets the following conditions: a stationary phase comprises a C18 chromatographic column; the mobile phase comprises a mobile phase A and a mobile phase B, and the mobile phase A comprises methanol or / and the mobile phase B comprises water; a gradient elution procedure is adopted. According to the method, the characteristic chromatogram reflecting the secondary metabolites of the isatis tinctoria seeds is constructed by adopting the ultra-high performance liquid chromatography, and a brand new reference is provided for evaluating the quality of the isatis tinctoria seeds.

The invention discloses a formula and a preparation process of a Chinese herbal medicine animal feed, and belongs to the technical field of animal feed preparation, the Chinese herbal medicine animal feed comprises the following raw materials in parts by weight: a core health-care formula: 20-30 parts of radix astragali, 15-25 parts of radix astragali, 10-20 parts of hawthorn, 10-20 parts of malt, 8-15 parts of pericarpium citri reticulatae, 15-20 parts of malt, 3-6 parts of fennel, 3-6 parts of anise and 3-6 parts of myrcia; and the immunological enhancement formula comprises 8 to 15 parts of honeysuckle flower, 5 to 12 parts of wild chrysanthemum flower, 8 to 15 parts of isatis root, 12 to 15 parts of lucid ganoderma, 6 to 10 parts of tsaoko amomum fruit, 15 to 20 parts of motherwort herb, 15 to 20 parts of medicated leaven and 15 to 20 parts of beautiful millettia root. According to the invention, a biochemical method of'synergistic extraction-concentration preparation 'is adopted, and active ingredients in the medicinal materials are extracted and concentrated to the greatest extent in advance in vitro, so that the herbal medicine raw materials are upgraded into high-concentration active extracts, and animals can directly, quickly and efficiently absorb the active ingredients, therefore, the additive amount is small, and the cost is low. And the effect is more obvious and quicker.