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Chicken gallbladder extraction composition and extraction method thereof

An extraction method and composition technology, which is applied in the field of chicken gall extract composition, can solve the problems of few types of amino acids, use more solvents, and low content, and achieve the effect of promoting choleresis, reducing surface tension, and high content

Inactive Publication Date: 2014-07-02
辽宁省药物研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method has many steps, uses many solvents, high cost, and low yield. In addition, the types of amino acids are few and the content is very low.

Method used

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  • Chicken gallbladder extraction composition and extraction method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Take 9.0kg of fresh chicken gall, freeze, chop, melt, squeeze, and filter out the bile; take chicken gall, add 90% ethanol, the volume of ethanol is 4 times the volume of chicken gall, stir at room temperature for 20min, place for 12h, precipitate protein , filtered to obtain the supernatant; get the supernatant and add 60# petroleum ether to extract 3 times, the volume of petroleum ether is 0.3 times of the supernatant, collect the ethanol layer, concentrate and evaporate to dryness to obtain 2.7648g of chicken gall extract composition, Based on fresh chicken guts, the yield is 30.7%. In the chicken gall extract composition: taurochenodeoxycholic acid accounts for 51%, other cholic acids account for 12%, amino acid 10%, protein 25%, fat 1%, and inorganic elements 1%. Among other cholic acids, taurocholic acid accounts for 75%, glycinolithocholic acid accounts for 15%, and allocholic acid accounts for 10%.

[0041] After testing, the chicken bile extract composition co...

Embodiment 2

[0044] Take 9.0kg of fresh chicken gall, freeze, chop, melt, squeeze, and filter out the bile; take the chicken gall, add 95% ethanol, the volume of ethanol is 3 times the volume of chicken gall, stir at room temperature for 30 minutes, place for 15 hours, and precipitate protein , filtered to obtain the supernatant; the supernatant was added to 90# petroleum ether for extraction 3 times, the volume of petroleum ether was 0.5 times that of the supernatant, the ethanol layer was collected, concentrated and evaporated to dryness to obtain 2.836g of chicken bile extract composition. Based on fresh chicken guts, the yield is 31.5%. In the chicken gall extract composition: taurochenodeoxycholic acid accounts for 45%, other cholic acids account for 15%, amino acid 15%, protein 23%, and fat 2%.

[0045] After testing, the chicken bile extract composition contains the following amino acids: aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine,...

Embodiment 3

[0047] Take 9.0kg of fresh chicken gall, freeze, chop, melt, squeeze, and filter out the bile; take chicken gall, add 98% ethanol, the volume of ethanol is twice the volume of chicken gall, stir at room temperature for 40min, place -17h, and precipitate Egg whites were filtered to obtain a supernatant; the supernatant was added to 90# petroleum ether to extract 3 times, the volume of petroleum ether was 1 times that of the supernatant, the ethanol layer was collected, concentrated and evaporated to dryness to obtain 2.891g of chicken gall extract composition . Based on fresh chicken guts, the yield is 32.1%. In the chicken bile extract composition: taurochenodeoxycholic acid accounts for 55%, other cholic acids account for 10%, amino acid 5%, protein 28%, fat 1.5%, and inorganic elements 0.5%. Among other cholic acids, taurocholic acid accounts for 80%, glycinolithocholic acid accounts for 10%, and allocholic acid accounts for 10%.

[0048] After testing, the chicken bile ex...

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Abstract

The present invention relates to a chicken gallbladder extraction composition, which comprises, by mass, 45-55% of taurochenodeoxycholic acid, 10-15% of other cholic acids, 5-15% of amino acids, 23-28% of proteins, and 0.5-2% of fat, wherein the chicken gallbladder extraction composition has the high taurochenodeoxycholic acid content, contains plural kinds of the amino acids, has the high amino acid content, has significant efficacy, and has high stability. The present invention further relates to an extraction method for the chicken gallbladder extraction composition. The extraction method comprises: freezing, chopping, melting and pressing chicken gallbladder, filtering to obtain the bile, adding ethanol to extract, standing, carrying out protein precipitation, filtering to obtain the supernatant, adding petroleum ether to extract, collecting the ethanol layer, and carrying out concentration evaporation dryness to obtain the chicken gallbladder extraction composition. The method has characteristics of simple process, easy operation, low cost and short purification period.

Description

technical field [0001] The invention relates to a chicken gall extract composition and an extraction method of the chicken gall extract composition. Background technique [0002] Chicken Gallbladder, mainly used for clearing heat and detoxifying; eliminating phlegm and relieving cough; improving eyesight. Mainly pertussis; chronic bronchitis; otitis media; infantile dysentery; sand shower; conjunctival congestion and tears; cataracts; postauricular eczema; hemorrhoids. Dosage: (1) Oral administration: take 1 to 3 fresh chicken gallbladders, take the juice and add sugar, or dry and grind into powder or make tablets, or make chicken gallbladder oral liquid; (2) External application: appropriate amount, take fresh chicken gallbladder Bile eyes. [0003] Chicken bile mainly contains 4 kinds of bile acids: 80% chenodeoxycholic acid, 17% cholic acid, 5% allocholic acid, all combined with taurine ) binding, 3β, 7α-dihydroxy-Δ4, 5-cholic acid (3β, 7α-dihydroxy-4,5-diene-cholic ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/413A61P1/16A61P1/14
Inventor 贾卫茹吴云飞赵鑫张勇
Owner 辽宁省药物研究院
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