45results about How to "Improve graft survival" patented technology

A tissue graft holder comprising a tray having a plurality of compartments for supporting tissue grafts and a hydrating solution, a channel connecting each compartment to an edge of the tray. The tray is rotatably mounted to a support which in turn is adapted for attachment to an appendage of a user. A dissecting cutting board to accommodate the graft trays and maintain tissue hydration during graft dissection.

A method is provided for administering to a mammal suffering from, or at risk for, multiple sclerosis, an initial dosing of a therapeutically effective amount of an anti-LFA-1 antibody, followed by a subsequent intermittent dosing of a therapeutically effective amount of the anti-LFA-1 antibody wherein the antibody is administered to the mammal no more than once per week in the intermittent dosing.

The present invention relates to methods and compositions for inhibiting pancreatic beta cell dysfunction and Fas-mediated apoptosis. The invention relates to recombinant vectors, including viral vectors, comprising nucleic acids molecules encoding inhibitors of interleukin-1beta (IL-1beta) and Fas-mediated apoptosis and the use of such vectors for transfer of said nucleic acid molecules into beta cells. The invention encompasses genetically engineered beta cells comprising nucleic acid molecules encoding inhibitors of IL-1beta signal transduction. The invention further relates to methods for transplanting such genetically engineered beta cells into a host recipient with a pancreatic disorder. The methods and compositions of the invention may be used to reduce Il-1beta mediated beta cell dysfunction and apoptosis, thereby reducing the insulitis associated with pancreatic disorders such as insulin dependent diabetes mellitus (IDDM).

The present invention relates to an antibody binding to human intercellular adhesion molecule-1 (ICAM-1) where the antibody is able to modulate the differentiation status of dendritic cells and prolong the graft survival. In addition, the present invention provides a pharmaceutical composition comprising the antibody, and method of using them for the treatment of disease.

An object of the present invention is to provide a pancreatic islet transplantation technique that enables increase of graft survival rate of pancreatic islets after pancreatic islet transplantation, to maintain survival of pancreatic islets, and to reduce the number of transplanted pancreatic islets required for normalizing blood glucose level. When performing pancreatic islet transplantation, by transplanting pancreatic islets and adipose tissue-derived stem cells together, it is possible to significantly improve graft survival rate of transplanted pancreatic islets, and reduce by half the number of transplanted pancreatic islets required for normalizing blood glucose level.

The invention relates to methods of modifying cells of corneal tissue to express an active agent, to modified corneal tissue, to vectors utilised in such methods and to methods of xeno- and allo-transplantation utilising the modified corneal tissue. The method of modifying cells of corneal tissue to express an active agent involves exposing harvested corneal tissue to an effective concentration for transfection of an expression vector which comprises a nucleotide sequence encoding for the active agent for a period sufficient to allow infection, such that cells of said the corneal tissue will express the active agent.

The present invention relates to tolerogenic mammalian dendritic cells (DCs) and methods for the production of the tolerogenic DCs. In addition, the present invention provides a method for enhancing tolerogenicity in a host comprising administering the tolerogenic mammalian DCs of the present invention to the host. The tolerogenic DCs of the present invention comprise an oligodeoxyribonucleotide (ODN) which has one or more NF-kappaB binding sites. The tolerogenic DCs of the present invention may further comprise a viral vector, and preferably an adenoviral vector, which does not affect the tolerogenicity of the tolerogenic DCs when present therein. Enhanced tolerogenicity in a host is useful for prolonging foreign graft survival and for treating inflammatory related diseases, such as autoimmune diseases.

A gene vector adapted for transient expression of a transgene in a peripheral organ cell comprising a regulatory sequence operably linked to a transgene wherein the regulatory sequence prevents or reduces expression of said transgene in hematopoietic lineage cells.

The present invention relates to the use of the Insulin-like growth factor-I (IGF-1) in immune modulation and / or in the treatment or prevention of pathogenic or aberrant immune responses or disorders and / or for use in the treatment or prevention of T-cell mediated disorders or diseases and / or for use in the treatment or prevention of diseases where the immune system contributes to the disease state.

The invention relates to the technical field of plant grafting, and particularly discloses a multi-station automatic grafting device which comprises a base frame, a grafting host, a vibrating conveying disk, a conveying line, a finished disk, a discharging component and a waste collecting component, wherein the grafting host, the vibrating conveying disk, the conveying line and the finished disk are mounted at the top of the base frame, the discharging component stretches over the finished disk on the conveying line, and the waste collecting component is mounted on the lower portion of the grafting host. The multi-station automatic grafting device avoids manual grafting operation, a dual-disk (a scion disk and a tree stock disk) grafting method is adopted, a feeding station, a shearing station and a grafting station in synchronous action are designed by the aid of the property of opposite rotating engagement of the two disks, the steps of feeding, cutting, grafting, grafting clamping and discharging are decomposed into every station, all the stations synchronously run by pipelined operation, grafting speed can reach 3 seconds per plant, efficiency is quite high, and the device hasextremely high controllability and operability.

The invention discloses a supporting device used for fruit tree grafting. The device comprises a fixing pile, a fixing base, a first buckle, a fixing hoop, an insertion rod, a bolt, a connecting rod,a hoop, a clamping bolt, sliding grooves, a sliding plate, a second buckle, a fixing plate, a hinge, a spring support, a sleeve, a supporting arm, a supporting rod, a rotary knob, a sliding block, a support, a bayonet lock, and a clamping groove. Beneficial effects of the device are that through arranging the two sliding grooves on the fixing base, and arranging the sliding plate which can slide on the sliding groove in a clamping manner, the sliding plate can be used to slidably adjust positions of the first buckle and the second buckle, the two buckles are ensured to respectively clamp and fix a scion and a stock, so as to ensure connection between the scion and the stock is more stable and firmer, and effectively improve grafting survival rate. Through welding the fixing hoop on the fixing plate, the fixing hoop is used to clamp and wrap a grafting joint, preventing a grafting position from getting wet, and effectively improving airtightness of the joint, and effectively improving grafting success rate.

The invention provides a grafting shear capable of straightly shearing stems. The grafting shear comprises a first rod body, a second rod body, a first seat plate, and a second seat plate, wherein the first rod body is provided with a first pivoting part; a first driving part extends from the front end of the first pivoting part, and a first linking part extends from the rear end of the first pivoting part; the second rod body is provided with a second pivoting part pivoted with the first pivoting part; a second driving part extends from the front end of the second pivoting part, and a secondlinking part extends from the rear end of the second pivoting part; the first seat plate is provided with a first connecting part pivoted with the first driving part of the first rod body; a first shear part extends from the front end of the first connecting part, and the rear end of the first connecting part is provided with a first guide part which, together with the second linking part of the second rod body, mutually guides and displaces; the second seat plate is provided with a second connecting part pivoted with the second driving part of the second rod body; a second shear part extendsfrom the front end of the second connecting part; and the rear end of the second connecting part is provided with a second guide part which, together with the first linking part of the first rod body, mutually guides and displaces. The grafting shear can improve the grafting survival rate.

The invention discloses a thin-shell pecan grafting method, and belongs to the technical field of pecan planting. The method includes the steps: firstly, cultivating thin-shell pecan seedlings, and taking the seedlings as grafted branches by the aid of high cell division ability of seedling stage; secondly, collecting terminal buds and first side buds of the seedlings with most auxin, mashing the terminal buds and the first side buds into slurry, mixing the slurry, peach gum and potassium nitrate to obtain concentrated solution with the auxin and film-forming ability, coating graft unions with the concentrated solution to form cell active layers, breaking through diaphragms generated by dead cell of the graft unions to form callus tissues, further differentiating the callus tissues to form new inward xylem and new outward phloem, performing sieve tube communication on the xylem and phloem of grafted seedlings, and communicating dredge tissues to improve graft healing ability. A film forming matter in the concentrated solution can form uniform protection film layers at grafted gaps, sap and water loss is prevented, infestation of germs on grafting wounds is avoided, and grafting survival rate is further increased.

The invention discloses a seedling grafting trimming device, and relates to the technical field of garden seedling raising. The trimming device comprises a fixing plate. The fixing plate is connectedwith a baffle by a screw and is in sliding fit with an adjusting block. One side of the adjusting block is in sliding fit with a cutter. The trimming device is provided with the fixing plate, the adjusting block with the cutter is arranged on the fixing plate, an inclined scion through hole is formed in the adjusting block, and the cutter can be used for cutting a scion and a rootstock simultaneously. The problem of low grafting survival rate caused by incomplete attaching of the scion section and the rootstock cut due to the fact that the cut and section angle of the scion and the rootstock is judged based on the subjective experiences of people is solved, and the problem of safety hazards caused by the fact that the hands are easy to injure by a grafting tool in the trimming and carryingprocess is solved.

The invention provides a grafting method for phyllanthus emblica. The method comprises the following steps: step a: selecting wild phyllanthus emblica as a rootstock; step b: obtaining a scion; and step c: when the sun shines again after rain in summer, subjecting the rootstock and the scion to bonding, and carrying out binding with a membrane. According to the method, the time that the sun shines again after rain is selected for grafting of the phyllanthus emblica in summer, so that the problems during grafting is reduced and the phyllanthus emblica is insusceptible to occurrence of mildew; meanwhile, the selection of the wild phyllanthus emblica as the rootstock improves the survival rate of grafted phyllanthus emblica.

The present invention relates generally to the field of tissue modification, and in particular, but not exclusively, to methods of modifying cells of ocular and particularly corneal tissue to produce immunoglobulins or immunoglobulin fragments. The invention also relates to modified ocular cells and tissues, to expression vectors utilised in such methods, to methods of xeno- and allo-transplantion using the modified tissue and to methods of therapy of ocular diseases and disorders. In a preferred embodiment of the invention there is provided a method of modifying cells of ocular tissue to produce an immunoglobulin or immunoglobulin fragment of interest, which comprises exposing ocular tissue to an effective concentration for transfection of an expression vector comprising a nucleotide sequence encoding for the immunoglobulin or immunoglobulin fragment, for a period sufficient to allow transfection, such that cells of said ocular tissue produce the immunoglobulin or immunoglobulin fragment.

The invention relates to special sealing wax for kiwi fruit tree branches and a preparation method of the special sealing wax. The special sealing wax for kiwi fruit tree branches comprises the following components in parts by weight: 80 to 120 parts of beewax, 50 to 100 parts of olive oil, 1 to 5 parts of Vaseline, 5 to 8 parts of a thickening agent and 75 to 120 parts of a sterilizing liquid. The special sealing wax for kiwi fruit tree branches is soft in texture, and can coat the ends of the kiwi fruit tree branches at normal temperature; the two ends of the kiwi fruit tree branches are sealed, so that moisture cannot be lost and the kiwi fruit tree branches are convenient to store, the ends of the kiwi fruit tree branches cannot be damaged, and the grafting survival rate is extremely high; and effective sterilization can be carried out on the ends of the kiwi fruit tree branches effectively by adding the sterilizing liquid, the ends are prevented from being rotted, the viscosity ofthe whole sealing wax can be increased through the thickening agent, and the sealing effect is improved.

The present invention provides compositions and methods for modulating B-lymphocyte activation. Nucleic acids encoding proteins and proteins so encoded which are capable of modulating B-lymphocyte activation are provided. Compositions and methods for the treatment of disorders related to dysfunction or dysregulation of B-lymphocyte activation are also provided. Prophylactics and methods for the prevention of such disorders are also provided. Also provided are compositions and methods for diagnostic and prognostic determination of such disorders. Further provided are assays for the identification of bioactive agents capable of modulating B-lymphocyte activation.

The present invention relates to a method for treating a subject having or at risk of a diabetes-related disorder. In a preferred embodiment, the method involves increasing the level or activity of Hypoxia Induced Factor 1 (HIF-1 α) in pancreatic-β-cells or insulin-sensitive tissues in the subject by administering to the subject an inhibitor of a protein that decreases the level or activity of HIF-1α. The present invention also relates to a method of transplanting pancreatic islet cells in a subject.

The present invention relates to an antibody binding to human intercellular adhesion molecule-1 (ICAM-1) where the antibody is able to modulate the differentiation status of dendritic cells and prolong the graft survival. In addition, the present invention provides a pharmaceutical composition comprising the antibody, and method of using them for the treatment of disease.

The invention discloses a Fengtang plum grafted seedling cultivating method, and belongs to the technical field of planting. By refrigerating a scion, hormone treatment is performed on the scion and rootstock in the grafting process, a specific substrate is added in a grafted seedling cultivation base, and specific fertilizer and water management is performed at a later stage. Wound healing can bepromoted, the graft survival rate is improved, a substrate has the functions of moisture retention and heat preservation to soil in use, the loss of other water and fertilizers can be prevented whilenutritional ingredients are provided, development of a root system in the growth process of grafted seedlings is promoted, branches are stronger, the survival rate of transplantation is higher, and the fruiting rate is higher.

The invention provides a grafting method for phyllanthus emblica. The method comprises the following steps: step a, selecting wild phyllanthus emblica as a rootstock; step b, obtaining a scion; and step c, taking the rootstock and the scion in the summer and the time that the sun shines again after the rain, performing joint, and performing binding by using a membrane. According to the grafting method provided by the invention, grafting time of the summer phyllanthus emblica is selected at the time that the sun shines again after the rain, so that reduction of problems when grafting is performed is facilitated, and mildewing is not easy to have; and the wild phyllanthus emblica is selected as the rootstock, so that a grafting survival rate of the phyllanthus emblica is improved.

The present invention relates to a method for treating a subject having or at risk of a diabetes-related disorder. In a preferred embodiment, the method involves increasing the level or activity of Hypoxia Induced Factor 1 (HIF-1 α) in pancreatic-β-cells or insulin-sensitive tissues in the subject by administering to the subject an inhibitor of a protein that decreases the level or activity of HIF-1α. The present invention also relates to a method of transplanting pancreatic islet cells in a subject.

The present invention relates to a pouch-like structure useful for mechanically preventing distension and / or resisting dilation of the heart and for supporting the hearts function by controllable and paracrine support of a failing heart in a mammal. The pouch-like structure is composed at least partly of engineered tissue comprising genetically engineered cells, such as genetically engineered cells other than cardiac myocytes whereby said genetically engineered cells contain a gene encoding a paracrine factor, said gene encoding the paracrine factor being under control of an inducible promoter system or a heterologous promoter system. Further, the present invention relates to a method for the preparation of the pouch-like structure for therapeutic, disease modelling, and drug development applications. In addition, the present invention relates to cells other than cardiac myocytes for use in the preparation of the pouch-like structure as described herein.

The invention provides a grafting method for Kiwi berry. The grafting method comprises the following steps: S1, selecting an old Kiwi berry tree in a grafting season, horizontally sawing off the trunkof the old Kiwi berry tree as a rootstock, and arranging a plurality of graft unions on the sawing plane of the trunk at intervals; S2, cutting Kiwi berry grafting branches into scions, and insertinga scion into a graft union in the step S1, allowing the scion to tightly bind with the cambium layer on the rootstock, and fixedly connecting the scion with the rootstock through an adhesive tape; and S3, sealing the rootstock and the exposed external part of the scion through grafting wax so as to complete grafting of the Kiwi berry. The grafting method for the Kiwi berry provided by the invention has rapid grafting speed and improves the efficiency of grafting.

A method for preparing a natural biological fertilizer for fruit trees in multiple seasons comprises the following steps: 1, a dry animal manure and fish pond sediment mixture, fungus breeding wastesand dry plant leaf stems are crushed for later use; 2, natural plants undergo water extraction to prepare a nutritional supplement solution mother liquor; 3, the supplement solution mother liquor is diluted 5-10 folds, and the diluted mother liquor, the dry animal manure, the fungus breeding wastes and dry plant leaf stems are mixed and uniformly stirred; 4, water is added to a product obtained instep 3, and closed environment fermentation is carried out; 5, a fermentation product obtained 50-90 days later is taken out and drained, and the obtained liquid is stored in a sealing tank in a sealing manner for 20-30 d to obtain a solution A; and 6, obtained residues and the fish pond sediment are uniformly stirred in a cement tank, the mouth of the tank is sealed with dry soil, and fermentation is carried out for 10-20 d to obtain a solid B. The water extraction step of the natural plants is characterized in that fresh tree leaves are mixed with water according to a ratio of 50:1 directlyor after being dried; and the fresh tree leaves are obtained after pruning. The natural biological fertilizer meets various needs of the fruit trees, increases the survival rate in the grafting period, and greatly shortens the breeding time.

The invention discloses a method for grafting Chinese rose plants by using Robinia plants as rootstocks. The method comprises the following steps of: A, carrying out artificial seedling cultivation onthe Robinia plants; B, grafting by adopting a scion grafting method such as splitting grafting, cutting grafting, side bark grafting, pith-cambium pairing grafting and the like or T-shaped budding, I-shaped budding, plate budding, sleeve grafting and the like; C, carrying out bud promoting and branch growing, pruning and branch bud management after grafting. The Robinia plants are used as the rootstocks to graft the Chinese rose plants, due to the graft compatibility of the Robinia plants and the Chinese rose plants, the grafting survival rate of the Chinese rose plants is improved, so that the growth rate of the Chinese rose plants is improved; meanwhile, the Robinia plants are extremely cheap, the Robinia plants grow fast, tree trunks are thick and strong, raw materials are easy to obtain, the Robinia plants are suitable for artificial cultivation, time for collecting a plurality of the raw materials in mountains is saved, the manpower cost is reduced, and the Chinese rose pants grafted by a locust tree can beautify streets to a great extent and generate good environmental benefits.

The invention relates to the field of apple cultivation, and provides a method for cultivating double-virus-free apple seedlings. The method includes the steps: S1, preparing virus-free rootstock seedlings and target variety seedlings; S2, performing rooting, domestication and transplanting culture on the virus-free rootstock seedlings and the target variety seedlings, wherein the virus-free rootstock seedlings are transplanted into a nursery garden in the domestication process, and the virus-free target variety seedlings are transplanted into a nutrition pot in the domestication process; andS3, grafting and culturing the double-virus-free apple seedlings, wherein the step 1 includes pretreatment of explants, stem tip culture, multiple times of high-temperature passivation and multiplication culture, and detoxification detection, and the step 1 is used for simultaneously treating the rootstocks and the target varieties. In conclusion, the rootstocks and the varieties subjected to detoxification treatment are grafted to obtain the required double-virus-free seedlings, so that the situation that the seedlings are non-toxic is effectively achieved, the propagation of soil-borne diseases of soil is inhibited, the resistance to diseases such as soil-borne rhizome rot and root rot is enhanced, thus the use of pesticides can be reduced, and conditions are created for producing greenpollution-free fruits.

The invention provides a propagation method of macadamia integrifolia. The propagation method comprises the following steps: (1), culturing rootstock seedlings: using tree planting bags for culturing seedlings, timely weeding, fertilizing, performing deinsectization, disease prevention and strengthening management and protection after the rootstock seedlings are cultured to be 60 cm or above, and selecting rootstock seedlings with the rootstock stem diameter of 0.95-1.35 cm as grafting seedlings; (2), selecting scions: the scions are semi-lignified spring twigs, retaining one intact leaf and shearing to remove the rest of leaves; (3), performing grafting: grafting is performed in late February during the spring, clipping lower half parts of the selected scions in the step 2 to achieve smooth wedge-shaped notches of 2-3 cm in length, longitudinally cutting centers of the notches of the rootstock to obtain grafting openings of 2.5-3 cm in length, inserting the scions with the wedge-shaped notches into the grafting openings for grafting; when the plants grow stably and new roots grow out of the plants, the plants can be transplanted out of a nursery; (4), performing field management: the plants that are transplanted out of the nursery are watered timely , weeds are killed, the fertilizer is applied and pest control is carried out. According to the propagation method of macadamia integrifolia, provided by the invention, rootstock seedlings, scions and the grafting method are optimally selected, so that the grafting survival percentage of macadamia integrifolia can reach 95% or above.