Method for cultivating double-virus-free apple seedlings

Abstract

The invention relates to the field of apple cultivation, and provides a method for cultivating double-virus-free apple seedlings. The method includes the steps: S1, preparing virus-free rootstock seedlings and target variety seedlings; S2, performing rooting, domestication and transplanting culture on the virus-free rootstock seedlings and the target variety seedlings, wherein the virus-free rootstock seedlings are transplanted into a nursery garden in the domestication process, and the virus-free target variety seedlings are transplanted into a nutrition pot in the domestication process; andS3, grafting and culturing the double-virus-free apple seedlings, wherein the step 1 includes pretreatment of explants, stem tip culture, multiple times of high-temperature passivation and multiplication culture, and detoxification detection, and the step 1 is used for simultaneously treating the rootstocks and the target varieties. In conclusion, the rootstocks and the varieties subjected to detoxification treatment are grafted to obtain the required double-virus-free seedlings, so that the situation that the seedlings are non-toxic is effectively achieved, the propagation of soil-borne diseases of soil is inhibited, the resistance to diseases such as soil-borne rhizome rot and root rot is enhanced, thus the use of pesticides can be reduced, and conditions are created for producing greenpollution-free fruits.

Description

technical field [0001] The invention relates to the field of apple cultivation, in particular to a method for cultivating double-detoxified apple seedlings. Background technique [0002] Apple, belonging to the bulk fruit of Rosaceae, is not only the most important fruit in my country, but also the most widely planted and produced fruit in the world; its taste is sweet and sour, and it is nutritious. [0003] In the existing apple planting, there are problems such as aging seedling varieties, underdeveloped root system, serious diseases and insect pests, and low fruiting rate. Studies from various countries have shown that most apple cultivars are infected with one or several viruses, among which apple chlorotic leaf spot virus, apple stem pox virus and apple stem groove virus are the most widely distributed and most harmful. Although the existing technology has researched various virus-free apple seedlings, it is not difficult to see that a large number of traditional graf...

AI Technical Summary

Problems solved by technology

Although the existing technology has researched various virus-free apple seedlings, it is not difficult to see that a large number of traditional grafted seedlings are used in China, while the virus-free apple seedlings in the current market are only The species detoxification treatment has been carried out, but the grafted rootstock is not detoxified. In this case, the detoxified apple seedlings will continue to be infected with the virus because the roots of the rootstock are poisonous, and it is difficult to truly realize the nontoxicity of the seedlings;