52 results about "Malus x domestica" patented technology

The invention relates to a hybridization seedling culturing method for malus spectabilis and apples and belongs to the technical field of seedling culturing. The malus spectabilis is adopted for the seedling culturing method. According to the method, the malus spectabilis is cultured to obtain malus spectabilis seedlings A as rootstocks; the malus spectabilis as female parents and the apples are hybridized to obtain hybridized seeds, and the seeds are planted to obtain malus spectabilis seedlings B; the malus spectabilis seedlings B are used for obtaining malus spectabilis and apple hybridized leaf bud pieces. A bud grafting method is adopted for grafting the obtained malus spectabilis and apple hybridized lead bud pieces on the malus spectabilis seedlings B to the malus spectabilis seedlings A. The hybridization seedling culturing method for the malus spectabilis and apples is short in seedling culturing period, high in sapling survival rate and adaptability of the obtained seedlings, rapid in growth and resistant to freezing, drought, waterlogging and barren after planting. Borne fruits through the obtained hybridized grafted seedlings not only have the taste of the apples, but also have the high-yield characteristic of the malus spectabilis and can rapidly enter a high-yield period, rapid popularization of a new species of apples is facilitated, and high economical benefits are high.

The invention discloses a method for positioning immune tissues of growth hormone for malus plants and application thereof. The method for positioning immune tissues of growth hormone for malus plants comprises the following steps of: (1) fixing plant tissues to obtain fixed plant tissues; (2) carrying out slice making on the fixed plant tissues on the basis of the step (1) to obtain a plant tissue slice; and (3) immunostaining the obtained plant tissue slice on the basis of the step (2), and determining the distribution condition of hormone in the plant tissues through observing a position of a positive signal. The method for positioning immune tissues of growth hormone for malus plants can eliminate the easily caused influence of nonspecific staining of woody plant tissues, and can identify the positive signal clearly. The method for positioning immune tissues of growth hormone for malus plants verifies that the growth hormone has tissue specificity when being distributed in the tissues of a stem tip or a root tip of an apple tree in a condition of iron deficiency.

The invention discloses a Malus sieversii (Ledeb.) Roem-derived plant growth-related protein, and a coding gene and application thereof. The protein provided by the invention is: a) a protein of which the amino acid sequence is shown as SEQ ID NO.2; or b) a protein which is obtained from SEQ ID NO. 2 through substitution and/or deletion and/or addition of one or more amino acid residues, is related to plant growth and is derived from a). The protein and the coding gene thereof can be used for regulating and controlling plant growth.

The invention discloses an apple flavone glycosyl transferase gene MdGT1. The apple flavone glycosyl transferase gene MdGT1 is derived from yellow leaves of malus baccata, the nucleotide sequence of the apple flavone glycosyl transferase gene MdGT1 is shown in SEQ. Id. NO.1, and the amino acid sequence coded by the nucleotide sequence of the apple flavone glycosyl transferase gene MdGT1 is shown in SEQ. Id. NO. 2. In-vitro enzymatic experiments and in-vivo transgenic apple callus experiments prove that when an anthocyanin substrate is added into the gene, anthocyanin metabolism is promoted, and the salt resistance of callus can be enhanced. The apple anthocyanin glycosyl transferase gene MdGT1 is identified for the first time, preliminary explanation is provided for the function of the apple anthocyanin glycosyl transferase gene MdGT1, and a theoretical and practical foundation is laid for selection and acquisition of apple germplasm resources in future.

The invention provides a method for constructing Saccharomyces cerevisiae for efficiently synthesizing alpha-amyrin, which belongs to the field of bioengineering. The method comprises the following steps: carrying out semi-rational design on alpha-amyrin synthase derived from Malus domestica, and carrying out saturated mutation and iterative mutation on key amino acid residue sites to obtain a series of mutants including an MdOSC1 mutant with the highest catalytic activity; then performing overexpression on key genes in an alpha-amyrin synthesis route, and meanwhile, performing overexpressionon diacylglycerol acyltransferase (DGA1) so that the storage capacity of alpha-amyrin in saccharomyces cerevisiae cells is improved. The yield of the saccharomyces cerevisiae engineering strain capable of producing alpha-amyrin at high yield constructed by the method reaches 213.79 mg/L, efficient production of alpha-amyrin in saccharomyces cerevisiae is realized, and the engineering strain has important significance in production.

The invention discloses bacillus licheniformis XNRB-3 and application thereof, and belongs to the technical field of agricultural microorganisms. The biological preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.15308 The strain provided by the invention has the functions of degrading phenolic acid substances such as phlorizin and antagonizing pathogenic bacteria, fermentation metabolites of the strain contain antifungal active substances and substances for promoting plant growth, alpha-bisabolol and 2, 4-di-tert-butylphenol have a remarkable inhibition effect on a plurality of pathogenic fungi, succinic acid has a remarkable inhibition effect on a plurality of pathogenic fungi, and the like. Monomethyl ester and dibutyl phthalate can promote root system elongation and lateral root development of arabidopsis thaliana. The strain can also be used for producing protease, cellulase, chitosanase, beta-1, 3-glucanase and the like; the bacillus subtilis secretes siderophores and auxin and has certain phosphorus dissolving, potassium dissolving and nitrogen fixing capacities. In pot culture and field tests, the addition of the strain XNRB-3 significantly promotes the growth of apple rootstock malus hupehensis seedlings and biennial apple saplings.

The invention discloses a Deletion fragment and its application in detecting the dwarfing of Malus plants. The invention provides a method for detecting the dwarfing ability of a rootstock of a Malus plant, comprising the steps of: detecting whether a deletion fragment is contained in the genome of a Malus plant to be tested, and the dwarfing ability of a rootstock of a Malus plant to be tested containing a Deletion fragment in the genome Higher than the Malus plant to be tested that does not contain a Deletion fragment in the genome; the Deletion fragment is the -1213th position to the -1172th position in the upstream direction of A in the initiation codon ATG of the Malus plant genome IPT5b gene ATG. Experiments of the present invention prove that the presence of the Deletion fragment is negatively correlated with dwarfing traits, and the absence and presence of the fragment can be used to quickly screen dwarf plants in offspring, which is of great significance in the field of plant dwarfism.

The invention discloses an EPSP (5-enolpyruvyl shikimate-3-phosphate) synthase multisite mutant from Malus domestica, and a coding gene and application of the mutant. The mutant has 8 mutation sites, the amino acid sequence of the mutant is as SEQ ID No. 1, and the base sequence of the coding gene of the mutant is as SEQ ID No. 2. Tests show that the EPSP synthase multisite mutant from Malus domestica and the coding gene thereof have high glyphosate resistance, high PEP (phosphoenolpyruvic acid) affinity is kept, and accordingly possibility for using the coding gene for cultivation of transgenic crops is provided.

The invention discloses an application of a plant water stress resistance related protein derived from malus sieversiana and a coding gene. The protein is named as MsSCL26 protein, and the protein canbe a protein with an amino acid sequence shown in SEQ ID No.1 in a sequence table. Experiments prove that compared with a plant without the MsSCL26 gene, the MsSCL26 overexpression transgenic plant has remarkable water stress resistance, and it is indicated that the protein MsSCL26 can improve the stress resistance of the plant.

The invention discloses an anti-wrinkle firming composition. The composition mainly contains tens of active ingredients, including lactobacillus/ soybean milk fermentation product filtrates, hydrolyzed Viola Tricolor extracts, euglena gracilis polysaccharides, malus domestica fructus cell culture extracts and the like, and the active ingredients are compounded with hydrolyzed collagen, glycerol, propanediol and the like. The anti-wrinkle firming composition can repair damaged skins, nourish skins, supplement skins with moisture, consolidate a repairing effect and accelerate skins to more favorably absorb the composition. After the composition is compounded, a proportion of irritant substances in the technical scheme is extremely low so as to be safe and reliable.

The invention relates to the technical field of plant disease prevention and control, in particular to application of polypeptide to preparation of a preparation for preventing and treating plant diseases; the preparation is prepared by taking polypeptide as an active component and adding auxiliary materials or auxiliary components. The preparation disclosed by the invention has obvious prevention and treatment effects on plant diseases caused by Erwinia amylovora, and has outstanding prevention and treatment effects on fire blight of rosaceae plants, especially economic crops such as pears, apples, haws and malus spectabilis. Compared with the prior art, the polypeptide preparation has the effects of wide action range, high safety, no biotoxicity, no drug resistance, no stimulation and no hormone, and can effectively prevent and treat plant diseases.

The invention discloses apple cultivation methods, particularly relates to an anti-ring-rot apple cultivation method, and belongs to the technical field of plant cultivation. The anti-ring-rot apple cultivation method is characterized in that biennial Malus hupenensis Rhed is used as the stock, Fuji apple with full branch buds is used as the scion, the Fuji apple scion is grafted at a 65-70cm position through side grafting, and then planting and conventional management are performed to obtain a tall spindle-shaped apple tree in the next year. By the cultivation method, tree vigor can be enhanced, germs can be inhibited, ring rot occurrence rate can be lowered, labor intensity can be lowered, labor efficiency can be increased, pesticide use times can be reduced, diseases and pests can be reduced, the rate of high-quality fruits is increased, early yield, early ripening, early formability and high disease resistance are achieved, and the problems of old orchard continuous cropping renewing and replanting diseases are solved.

The invention discloses an agent used for preventing and treating aphis pomi of malus halliana koehne. The agent comprises the following components in parts by weight: 20-28 parts of 30% rotenone emulsifiable concentrate, 5-8 parts of rabdosia serra powder, 4-9 parts of lignosulfonate, 3-8 parts of saponin powder, 3-8 parts of 1,2-butanediol, 18-25 parts of butyl acetate, 4-8 parts of alkyl phenyl polyethyl ether, 4-9 parts of castor oil, 8-13 parts of rosin powder, 8-14 parts of polybutylene, 3-7 parts of sodium fluosilicate, 7-10 parts of sodium dodecyl benzene sulfonate and 30-38 parts of water. A use method comprises the following steps: proportionally allocating and mixing the components and stirring the components uniformly to obtain the agent for prevention and treatment; then spraying the agent around affected plants after diluting the agent for prevention and treatment. The agent has good prevention effects, is convenient to use, reduces the pesticide consumption, reduces environmental pollution and is beneficial to ecological balance.

The invention provides a malus pumila Rhodomyrtus tomentosa fruit vinegar beverage and a preparation method thereof. The malus pumila Rhodomyrtus tomentosa fruit vinegar beverage comprises the following components by weight: 5-15 parts of a Rhodomyrtus tomentosa fruit vinegar stock solution, 20-45 parts of malus pumila juice, 3-15 parts of a sweetener, and 8-15 parts of calcium lactate. The preparation method includes: firstly preparing the Rhodomyrtus tomentosa fruit vinegar stock solution, then mixing the components, conducting boiling and filtering, and finally carrying out homogenizing, sterilizing and packaging to obtain a finished product. The malus pumila Rhodomyrtus tomentosa fruit vinegar beverage provided by the invention not only has unique taste and rich nutrition, but also has the effects of promoting digestion, increasing appetite, maintaining beauty and keeping young, helping produce saliva and slake thirst, and eliminating fatigue.

The invention relates to a method for detecting expression quantity of miR156 of malus sieversii. The method comprises the following steps: (1) extracting total RNA; (2) inversely transcribing; (3) carrying out qRT-PCR detection; and (4) analyzing data obtained by qRT-PCR by using a method as shown in the specification, and making a histogram in an excel chart to obtain the relative expression quantity of targeted gene miR156 of the malus sieversii. The invention provides the novel method for extracting small RNA of the malus sieversii for the first time. The method can provide reference for detection of expression conditions of small RNA of ligneous plants, has reference significance for research of a phase transformation regulation and control net during vegetative growth and reproductive growth, and meanwhile, can also provide theoretical basis for crossbreeding and genetic improvement practice of the malus sieversii in the later period.

The invention discloses a grafting cultivation method suitable for dwarf interstock apple seedlings of apples in alpine regions belonging to the technical field of apple grafting cultivation. The method comprises the three steps of seedling rootstock cultivation, dwarf interstock grafting and bud grafting of cultivated varieties, and specifically comprises the following steps of preparing malus baccata seeds from January to February of the first year for germination accelerating treatment; sowing the malus baccata seeds in a greenhouse after germination accelerating treatment; after the malus baccata seeds germinate, screening out qualified malus baccata nursery stocks and transplanting outdoors; purchasing MM106 dwarfing rootstocks in September of the first year, grafting with malus baccata; and from August to September of the second year, selecting scions of a cultivated variety with the tree age of 4 to 6 years, treating the scions for 1 to 2 days, then grafting buds to dwarf interstock seedlings, and spraying a plant growth regulator after 10 to 15 days. The survival rate of the dwarf interstock apple seedlings obtained through the method in the alpine regions reaches 96 percent or above, and the seedling raising cost can be reduced.

The invention discloses a rose glacier spring rejuvenating facial mask formula which comprises spring, rose hydrosol and various moisturizing and repairing ingredients. The various moisturizing and repairing ingredients specifically include: a main active ingredients and common ingredients; the main active ingredient is MetaTrixAPL-3 (first-generation stem cell products: MALUS DOMESTICA fruit cellculture extract, FRAGARIA VESCA fruit extract, GLYCINE SOJA seed extract, a hydrolyzed collagen, water, butanediol and 1,2-hexanediol; and effects: moisturization, wrinkle resistance and repairing).Specific effects of the ingredients in the rose glacier spring rejuvenating facial mask formula are that: the MALUS DOMESTICA fruit cell culture extract, the FRAGARIA VESCA fruit extract, the GLYCINESOJA seed extract, the hydrolyzed collagen, the water, the butanediol and the 1,2-hexanediol have effects of moisturizing, resisting to wrinkles and repairing; small molecule sodium hyaluronate has effects of moisturizing and resisting to wrinkles; sodium polyglutamate has effects of moisturizing and improving skin elasticity; and schizophyllin has effects of resisting to allergy, promoting woundhealing, resisting to ageing and moisturizing.

The invention discloses an apple MdSHN1 gene, the nucleotide sequence of the apple MdSHN1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the protein is as shown in SEQ ID NO.2. According to the invention, a cDNA full-length sequence of the MdSHN1 gene is cloned from a waterlogging-resistant apple rootstock Malus hupehensis leaf, a primer for amplifying the apple MdSHN1 gene is also disclosed, and an amino acid sequence of the gene is determined. Through arabidopsis thaliana transgenosis function verification and analysis, it is found that the MdSHN1 gene has a remarkable effect in the aspect of improving the resistance of a plant, the adversity resistance of the transgenic plant is improved, particularly, compared with a wild type, the tolerance of the transgenic plant is higher than that of the wild type under flooding stress, and it can be seen that the MdSHN1 gene is related to waterlogging tolerance, the waterlogging tolerance of the plant can be improved, and the stress resistance of the transgenic plant is improved. The method is of great significance to breeding of new apple varieties.

The invention discloses a method for rapid rooting and root growth promotion of malus plants in hydroponics, which comprises the following steps of: selecting a proper nutrient solution, transplanting in a proper period, properly cutting roots, ventilating the nutrient solution at regular time, recovering seedlings in weak light, controlling a culture environment and the like, so that the transplanted and hydroponic malus plants can grow new roots early; moreover, the root system growth is better, and a more effective culture method is provided for researching root system development, stress physiology, molecular regulation and control mechanisms and the like of the malus plants.

The invention discloses an application of an R1R6 genotype apple as a receptor material in preparation of a red pulp transgenic apple, provides application of an R1R6 genotype apple as a receptor material in preparation of a transgenic apple, provides an application of the apple (Malus sp.) BCF2 as an acceptor material in preparation of a transgenic apple, and also provides a method for preparing the transgenic apple, which comprises the following step: performing genetic transformation on the Malus sp. BCF2 serving as a receptor material to obtain the transgenic apple. The inventor of the invention finds that in an F2 generation obtained by taking the apples of "purplish red No.1" and "Gala" varieties parents, and it is found that a strain with a genotype of R1R6 is easier to obtain a tissue culture seedling and has higher transformation efficiency as a genetic transformation receptor, the transformation rate of a G2 strain being the highest. The method disclosed by the invention has an important application value for the creation of the transgenic red-pulp apples.