Malus sieversii (Ledeb.) Roem-derived plant growth-related protein, and coding gene and application thereof

A plant growth and protein technology, which is applied to the plant growth-related protein and its coding gene and application field from wild apples in Xinjiang, which can solve the problems of difficult improvement of fruit quality, low land utilization rate, poor early fruit and high yield, etc.

Active Publication Date: 2013-06-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The land utilization rate is low, the early fruit and high yield are poor, and the fruit quality is not easy to improve

Method used

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  • Malus sieversii (Ledeb.) Roem-derived plant growth-related protein, and coding gene and application thereof
  • Malus sieversii (Ledeb.) Roem-derived plant growth-related protein, and coding gene and application thereof
  • Malus sieversii (Ledeb.) Roem-derived plant growth-related protein, and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Embodiment 1, the cloning of MdGH3-1 gene and the construction of transgenic vector

[0078] 1. Cloning of MdGH3-1 gene

[0079] Using Xinjiang wild apple (Malus sieversii (Ledeb.) Roem.) hydroponic seedlings as materials, total RNA was extracted from its leaves by CTAB method, and RNA was used as a template to reverse transcribe into cDNA. Using cDNA as a template, the full-length cDNA of MdGH3-1 was amplified by RT-PCR method. After the PCR product was recovered, A reaction was performed at the end, and the A product was added for purification and then connected to the pMD18-T cloning vector to obtain the plasmid pMD18-T / MdGH3-1 was transformed into Escherichia coli DH5α competent cells and sent to the company for sequencing. Wherein, RT-PCR primers are shown in Table 2.

[0080] Table 2. RT-PCR primers

[0081] Primer name Primer sequence MdGH3-1-L 5′-CAGTGAGTGACGTCCTAGAATCG-3′ MdGH3-1I-R 5′-CCAAATCCAACATACACAATGAGAG-3′

[0082] Amo...

Embodiment 2

[0089] Embodiment 2, cultivating trans MdGH3-1 gene Arabidopsis

[0090] The experiments in this example prove that the transgenic Arabidopsis thaliana that introduces the encoding gene of MdGH3-1 shown in the 75th-1898 nucleotides of SEQ ID No. 2 has the following A to E compared with the recipient Arabidopsis traits: A, the number of lateral roots of the transgenic plant is reduced compared with the recipient plant; B, the transgenic plant is smaller than the recipient plant; C, the transgenic plant is compared with the recipient plant Compared with the recipient plant, the plant height becomes smaller; D, compared with the recipient plant, the flower shoot height of the transgenic plant decreases; E, the transgenic plant, compared with the recipient plant, the flower shoot number increases . It shows that MdGH3-1 and its coding gene can be used to regulate plant growth. The specific experimental methods and experimental results are as follows:

[0091] 1. Obtaining trans...

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Abstract

The invention discloses a Malus sieversii (Ledeb.) Roem-derived plant growth-related protein, and a coding gene and application thereof. The protein provided by the invention is: a) a protein of which the amino acid sequence is shown as SEQ ID NO.2; or b) a protein which is obtained from SEQ ID NO. 2 through substitution and/or deletion and/or addition of one or more amino acid residues, is related to plant growth and is derived from a). The protein and the coding gene thereof can be used for regulating and controlling plant growth.

Description

technical field [0001] The invention relates to a protein related to plant growth derived from Xinjiang wild apple, its coding gene and application. Background technique [0002] Apple is the largest fruit tree species in my country. Since the mid-1990s, its cultivated area and output have been ranked first in the world. By 2011, the cultivated area was 32.8 million mu and the output was 31.5 million tons, respectively accounting for 52% of the world's cultivated area and 48% of the output, and the export volume accounted for 12% of the global apple trade volume. The traditional apple cultivation methods in my country are big crown sparse planting and arbor stock cultivation. The land utilization rate is low, the early fruit yield is poor, and the fruit quality is not easy to improve. Since the M-series apple dwarf rootstocks were bred in the UK in 1917, the production practice has proved that compared with the close planting of arbor stock, the close planting of dwarf sto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N1/13C12N1/15C12N1/19C12N1/21C12N5/10A01H5/00
Inventor 李天红袁华招刘芸沈欣杰廖雄王琪刘琳琳
Owner CHINA AGRI UNIV
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