Malus sieversii (Ledeb.) Roem-derived plant growth-related protein, and coding gene and application thereof
A plant growth and protein technology, which is applied to the plant growth-related protein and its coding gene and application field from wild apples in Xinjiang, which can solve the problems of difficult improvement of fruit quality, low land utilization rate, poor early fruit and high yield, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0077] Embodiment 1, the cloning of MdGH3-1 gene and the construction of transgenic vector
[0078] 1. Cloning of MdGH3-1 gene
[0079] Using Xinjiang wild apple (Malus sieversii (Ledeb.) Roem.) hydroponic seedlings as materials, total RNA was extracted from its leaves by CTAB method, and RNA was used as a template to reverse transcribe into cDNA. Using cDNA as a template, the full-length cDNA of MdGH3-1 was amplified by RT-PCR method. After the PCR product was recovered, A reaction was performed at the end, and the A product was added for purification and then connected to the pMD18-T cloning vector to obtain the plasmid pMD18-T / MdGH3-1 was transformed into Escherichia coli DH5α competent cells and sent to the company for sequencing. Wherein, RT-PCR primers are shown in Table 2.
[0080] Table 2. RT-PCR primers
[0081] Primer name Primer sequence MdGH3-1-L 5′-CAGTGAGTGACGTCCTAGAATCG-3′ MdGH3-1I-R 5′-CCAAATCCAACATACACAATGAGAG-3′
[0082] Amo...
Embodiment 2
[0089] Embodiment 2, cultivating trans MdGH3-1 gene Arabidopsis
[0090] The experiments in this example prove that the transgenic Arabidopsis thaliana that introduces the encoding gene of MdGH3-1 shown in the 75th-1898 nucleotides of SEQ ID No. 2 has the following A to E compared with the recipient Arabidopsis traits: A, the number of lateral roots of the transgenic plant is reduced compared with the recipient plant; B, the transgenic plant is smaller than the recipient plant; C, the transgenic plant is compared with the recipient plant Compared with the recipient plant, the plant height becomes smaller; D, compared with the recipient plant, the flower shoot height of the transgenic plant decreases; E, the transgenic plant, compared with the recipient plant, the flower shoot number increases . It shows that MdGH3-1 and its coding gene can be used to regulate plant growth. The specific experimental methods and experimental results are as follows:
[0091] 1. Obtaining trans...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com