Bacillus licheniformis XNRB-3 and application thereof

A technology of Bacillus licheniformis and inoculum, which is applied in the field of agricultural microorganisms, can solve the problem of less Bacillus biological control agents, etc., and achieves the effects of reducing the obstacles of continuous cropping of apples, promoting growth and excellent effects.

Active Publication Date: 2022-04-29
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, relatively few Bacillus biocontrol agents are available for the control of ARD

Method used

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  • Bacillus licheniformis XNRB-3 and application thereof
  • Bacillus licheniformis XNRB-3 and application thereof
  • Bacillus licheniformis XNRB-3 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1: Isolation and identification of bacterial strains

[0083] 1. Isolation and purification of strains:

[0084] The roots of healthy and vigorous apples were selected from replanted orchards in Jincheng City, Shanxi Province by the improved sticking root method, and the endogenous antagonistic bacteria were isolated and screened immediately. First rinse the roots with tap water for 5 minutes to remove the adhered soil particles, then immerse in 75% ethanol for 2 minutes for surface sterilization, then rinse with sterile water for 3 times, then soak in 5% sodium hypochlorite solution for 5 minutes, and finally rinse with sterile water Rinse 5 times. After proper drying, the surface-sterilized roots were cut into 1-cm root segments and transferred to LB medium to isolate endophytic bacteria. Four roots were placed in each Petri dish, and the Petri dish was kept at 37 °C for one week. In addition, apply the sterile water used to rinse the root system for the las...

Embodiment 2

[0108] Example 2: Optimization of fermentation process of strain XNRB-3 and preparation of fermentation-related products

[0109] 1. Optimization of liquid fermentation conditions:

[0110] The determination of the bacterial fermentation liquid adopts the turbidimetric method, that is, the fermentation liquid 10-15mL is centrifuged at 2000r / min for 2min, and the OD of the bacterial liquid is measured.600 value (the absorbance value of the solution at 600nm wavelength), combined with the results of single factor experiments and orthogonal experiments, Plackett Burman and Box Behnken optimized the composition of Bacillus licheniformis XNRB-3 liquid fermentation medium in response surface analysis (RSM) and fermentation conditions.

[0111] After optimization, the best liquid fermentation conditions of strain XNRB-3 are:

[0112] The composition of liquid fermentation medium: sucrose 21.03g / L, beef extract 8.54g / L, MgSO 4 1.0g / L, K 2 HPO 4 1.5g / L, KCl 1.0g / L; pH 7.5.

[011...

Embodiment 3

[0121] Example 3: Functional identification of strain XNRB-3 and fermentation-related products

[0122] 1. Broad-spectrum antibacterial effect of strain XNRB-3:

[0123] In order to further verify whether the XNRB-3 strain has a broad-spectrum antibacterial effect on plant-derived diseases, another four common pathogenic fungi were selected for further antibacterial tests. The result is as Figure 5 shown.

[0124] The results of confrontation test showed that XNRB-3 strain had a strong inhibitory effect on the growth of 4 kinds of pathogenic Fusarium mycelium. It also has a strong inhibitory effect on Phytophthora, Rhizoctonia solani, Alternaria and apple tree rot. Therefore, the strain XNRB-3 has a broad-spectrum antibacterial effect on plant pathogenic bacteria under the conditions of the in vitro petri dish test.

[0125] 2. Effects of fermentation-related products of strain XNRB-3 on the germination of Fusarium oxysporum spores:

[0126] The effect of the fermentatio...

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Abstract

The invention discloses bacillus licheniformis XNRB-3 and application thereof, and belongs to the technical field of agricultural microorganisms. The biological preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.15308 The strain provided by the invention has the functions of degrading phenolic acid substances such as phlorizin and antagonizing pathogenic bacteria, fermentation metabolites of the strain contain antifungal active substances and substances for promoting plant growth, alpha-bisabolol and 2, 4-di-tert-butylphenol have a remarkable inhibition effect on a plurality of pathogenic fungi, succinic acid has a remarkable inhibition effect on a plurality of pathogenic fungi, and the like. Monomethyl ester and dibutyl phthalate can promote root system elongation and lateral root development of arabidopsis thaliana. The strain can also be used for producing protease, cellulase, chitosanase, beta-1, 3-glucanase and the like; the bacillus subtilis secretes siderophores and auxin and has certain phosphorus dissolving, potassium dissolving and nitrogen fixing capacities. In pot culture and field tests, the addition of the strain XNRB-3 significantly promotes the growth of apple rootstock malus hupehensis seedlings and biennial apple saplings.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a bacillus licheniformis XNRB-3 and its application. Background technique [0002] Apple replanting disease (ARD) is a soil disease caused by biotic and abiotic factors. It occurs in major apple growing regions around the world and poses a major threat to the development of the apple industry. More and more studies have shown that biological factors are the main cause of ARD, mainly including: nematodes, fungi (Rhizoctonia solani, Geocarp, Fusarium, Alternaria and Cylindrosporium), egg Fungi (Pythium, Phytophthora). Abiotic factors may also play an important role in cropping obstacles, such as soil physicochemical properties, nutrient elements, accumulation of phenolic compounds or phytotoxins in diseased roots. [0003] Previous studies have shown that Fusarium is one of the main pathogens causing ARD in China, South Africa and Italy. Currently, chemical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G7/06A01G17/00A01N63/22A01P3/00C12R1/10
CPCC12N1/20A01N63/22A01G7/06A01G17/005
Inventor 尹承苗段亚楠毛志泉刘鑫吕毅陈学森沈向
Owner SHANDONG AGRICULTURAL UNIVERSITY
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