Method and special primer pair for detecting quality character of pork

A pork quality and primer pair technology, which is applied in DNA/RNA fragments, recombinant DNA technology, animal husbandry, etc., can solve the problems of pork quality decline and achieve the effects of reducing breeding costs, low cost, and simple operation

Inactive Publication Date: 2012-05-30
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0002] For a long time, the world's pig breeding work has mainly focused on the improvement of growth rate, feed utilization rate, and lean meat rate, but while making significant progress, it has also led to a decline in pork quality

Method used

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  • Method and special primer pair for detecting quality character of pork
  • Method and special primer pair for detecting quality character of pork
  • Method and special primer pair for detecting quality character of pork

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Experimental program
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Embodiment 1

[0031] Embodiment 1, the identification of pork quality character

[0032] 1. Determination of genetic polymorphism sites

[0033] 1. PCR amplification

[0034] Primer pairs are as follows:

[0035] U (upstream primer): 5'-TGCCTGCTCCTTAAGCTTCT-3' (sequence 1 of the sequence listing);

[0036] D (downstream primer): 5'-GGGAGGGAGGAAAACAAGAG-3' (SEQ ID NO: 2 of the Sequence Listing).

[0037] Using the genomic DNA of 3 Damin hybrid pigs as templates, PCR amplification was performed using primers U and D.

[0038] Amplification system: Genomic DNA 200ng, 10×PCR amplification buffer 5μl, dNTPs 10mM, upstream and downstream primers 50ng each, Taq DNA polymerase 0.75U, Mg 2+ 2.5mmol / L, with ddH 2 O Supplement the reaction system to 50 μl.

[0039] The PCR reaction conditions were: denaturation at 95°C for 5 min; then denaturation at 95°C for 20 s, annealing at 58°C for 30 s, and extension at 72°C for 30 s, a total of 35 cycles; finally, extension at 72°C for 10 min.

[0040] ...

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Abstract

The invention discloses a method and a special primer pair for detecting the quality character of pork. The auxiliary method for identifying the quality character of pork comprises the following steps of: detecting whether the 289th nucleotide from the 5' tail end of a DNA fragment shown by SEQ ID NO:1 in the genome of a pig to be detected is T or C; if the 289th nucleotide is T, then marking the genotype of the pig to be detected as TT; and if the 289th nucleotide is C, then marking the genotype of the pig to be detected as CC. The quality of the pig with the genotype TT is superior to that of the pig with the genotype CC. The method can rapidly and accurately identify the genotype of the pig, and the quality of the pork of the pig to be detected is judged. When used for pig breeding, the method is beneficial to accelerating the breeding process. Thus, the method has broad application prospect in pig breeding.

Description

technical field [0001] The invention relates to a method for detecting pork quality traits and a pair of special primers. Background technique [0002] For a long time, the world's pig breeding work has mainly focused on the improvement of growth rate, feed utilization rate, and lean meat rate, but while making significant progress, it has also led to a decline in pork quality. With the improvement of people's material living standards, people's demand for pork is no longer only the pursuit of quantity, but also higher and higher requirements for pork quality. Therefore, the focus of pig breeding work has gradually shifted to the improvement of pork quality traits. [0003] Marbling, tenderness and juiciness directly affect the appearance of pork, and these apparent traits directly affect consumers' desire to buy pork. The fat content in pork, especially the intramuscular fat content, directly affects the taste. Studies have shown that intramuscular fat (IMF) is directly ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11A01K67/027
Inventor 张龙超王立贤颜华王立刚
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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