The invention discloses a swine
gene expression
muscle creatine kinase (MCK)-diacylglycerol acyltrabsferase 1(DGAT1) carrier and a preparation method thereof. The preparation method includes a first step of obtaining a
complementary deoxyribonucleic acid (c
DNA) overall length sequence of a swine DGAT1
gene according to the DGAT1
gene to compound the gene, a second step of designing two pairs of two-end distinguished sequences of two ends of a specific primer augmentation swine MCK gene
promoter, enabling the two-end distinguished sequences to be connected on a p
enhanced green fluorescent protein (p EGFP)-N1 carrier, obtaining the complete
promoter of the swine MCK gene according to gene capture technology, wherein the overall length of a
promoter sequence is 7121bp, and a third step of using the swine DGAT1 gene to replace a
green fluorescent protein (GFP) gene on a p EGFP-N1-MCK carrier, and completing the structure of the MCK-DGAT1 expression carrier. The experimental result indicates that the expression carrier structured by the swine DGAT1 gene and the
muscle specific
expression gene MCK promoter, the contents of
triglyceride and aliphatic acid in animal individual muscles are remarkably increased, and reliability and authenticity of the experiment are improved. The verified expression carrier is directly applied to swine transgenic
engineering and
molecular breeding. Through transgenosis, new species of high
intramuscular fat can be obtained, quality of pork is improved, and the time for conventional breeding cultivating high intramuscular adipose is shortened.