124 results about "Swine herd" patented technology

The invention relates to the technical field of livestock breeding, in particular to an intelligent feed optimal proportioning method based on pig herd nutrition analysis, which comprises the following steps: S1, collecting stress index data of a pig herd; s2, analyzing the stress level and the stress source of the swinery; s3, dynamically adjusting nutritional requirement parameters according to the stress level and the stress source, and determining nutrients needing to be adjusted under different stress conditions; s4, making corresponding stress mitigation measures based on the stress source and the stress intensity; s5, generating an optimal feed formula meeting the nutritional requirements of the swinery under the stress condition; and S6, converting the optimal feed formula into a production instruction, and sending the production instruction to feed production equipment. By dynamically adjusting the nutritional requirements of the swinery under different stress conditions and combining intelligent feed production equipment, precise feed formula optimization and efficient production are achieved, the feed utilization rate and the swinery health level are improved, and meanwhile the breeding cost is reduced.

The invention relates to the technical field of agricultural science, and discloses a low-purine ecological pig circulating breeding system which comprises a breeding environment regulation and control module, a low-purine feed management module, an excrement treatment module, a recycling module, a health monitoring module, an intelligent decision-making module, a traceability management module, a water quality purification module and an energy recovery module. The breeding environment regulation and control module monitors in real time and automatically regulates pig house environment parameters through a distributed sensor network; the low-purine feed management module generates a low-purine feed formula based on growth stage recognition and nutritional requirement analysis; the excrement treatment module is used for converting breeding wastes into organic fertilizer raw materials; the cyclic utilization module realizes precise returning of the organic fertilizer to the field according to planting requirements; the health monitoring module evaluates the purine level of the swinery; the intelligent decision-making module integrates the multi-source data to generate a comprehensive optimization scheme; and the traceability management module records whole-process data through a block chain technology. The stability of low-purine culture is guaranteed, and the success rate of low-purine culture is increased.

The invention relates to a primer of an RBP4 gene molecular marker related to pig weak litter number character and application of the primer, and belongs to the technical field of biology. The RBP4 gene molecular marker is located at the 105043789th base of chromosome 14 of a pig reference genome Sscrofa11.1 version, the base is mutated into A or G, and the genotypes are A / A, A / G and G / G. The number of weak piglets of the G / G genotype pigs is lower than that of the A / G and A / A genotype individuals, and the number of weak piglets of the A / G genotype pigs is lower than that of the A / A genotype individuals. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker is used as a genetic marker for pig breeding to breed pigs with less weak piglets; the method can efficiently and accurately detect the weak piglet number character, improves the survival rate of healthy piglets of a pig herd, and has an important value for breeding and production of pigs.

The invention discloses a device for disease monitoring of swinery and a computer readable storage medium. The apparatus comprises: a processor; the memory is used for storing a program instruction for performing disease monitoring on the swinery, and when the program instruction is executed by the processor, the device realizes the following operations: acquiring basic information of a target swinery and a target image containing the target swinery; inputting the target image into a preset model for attitude detection operation, so as to output the attitude of each pig as an attitude detection result of the target pig herd; based on the posture detection result, determining a first proportion of the pigs whose postures are the target abnormal postures in the target swinery, and based on the first proportion, determining a disease state of the target swinery; and determining a treatment scheme of the target swinery based on the day age, the weight and the illness state. By means of the scheme, the diseases causing the abnormal postures of the swinery can be recognized in time, a reasonable treatment scheme is given, and therefore the health risk of the swinery is reduced.

The invention relates to the technical field of animal husbandry, and discloses an intelligent building pig farm feed control management method and system based on the Internet of Things, and the method comprises the steps: extracting pig herd behavior track data and pig herd state data in pig herd activity behavior data, and analyzing the pig herd activeness and pig herd abnormal behaviors corresponding to a building pig farm; analyzing the health state of the swinery in the building pig farm, analyzing the environment-ingestion correlation effect of the building pig farm, and analyzing the feed demand trend of the building pig farm; recording feed tower remaining data of the building pig farm, calculating a feed consumption rate corresponding to the building pig farm based on the feed tower remaining data, and setting a layered feeding rule of the building pig farm; and calculating the pig group fat dispersion of the building pig farm, determining the feed demand quota of the building pig farm, and executing feed control management on the building pig farm by using the Internet of Things management and control equipment to obtain a management result. The accuracy of intelligent building pig farm feed control management can be improved.

The invention belongs to the technical field of intelligent breeding and robots, and particularly relates to a pig house robot health monitoring and early warning system for a pig raising platform. The system aims to solve the problem of early warning lag caused by discontinuity of existing monitoring data. The system comprises a static sentinel node subsystem which is used for continuously collecting data at high frequency, generating an abnormal score and sending a triage request; the mobile diagnosis and service unit subsystem is used for performing autonomous diagnosis after receiving the request, acquiring individual accurate data and generating a report; and the central control and prediction subsystem is used for fusing data, constructing a space-time atlas model, predicting epidemic disease transmission and generating prospective early warning. By means of the system, 7 * 24-hour uninterrupted monitoring of swinery is achieved, data faults are solved, common screening-fine diagnosis cooperation is formed, a space-time diagram neural network is used for providing prospective early warning, and an epidemic disease prevention and control gateway is remarkably advanced.

The invention belongs to the technical field of immunological detection, and particularly relates to an antibody for resisting porcine pseudorabies virus glycoprotein gB and application of the antibody. The antibody is a first antibody or a second antibody, amino acid sequences of light chains CDR1-3 of the first antibody are respectively shown as SEQ ID NO.3-5, and amino acid sequences of heavy chains CDR1-3 of the first antibody are respectively shown as SEQ ID NO.8-10; the amino acid sequences of light chains CDR1-3 of the second antibody are respectively as shown in SEQ ID NO.13-15, and the amino acid sequences of heavy chains CDR1-3 of the second antibody are respectively as shown in SEQ ID NO.18-20. The antibody and the PRV glycoprotein gB have good reactivity, recognition specificity and binding sensitivity, the detection sensitivity and accuracy of the gB protein or the anti-gB protein antibody are improved, and an antibody tool with excellent performance is provided for qualitatively or quantitatively evaluating the immune effect of swine herd vaccines and detecting the content of porcine pseudorabies virus antigens in a sample.

The invention relates to the field of breeding management, and discloses an intelligent breeding environment data analysis method and system based on dynamic perception, and the method comprises the steps: dynamically collecting pig herd data through a mobile multi-mode perception terminal, and carrying out the data correction and segmentation through an anti-interference algorithm, so as to improve the accuracy; then, constructing a deep learning model, and fusing the physiological behaviors and the environmental data to generate a pig herd comprehensive comfort index; and finally, active regulation and control are realized through time sequence prediction, and instructions are issued to form a closed loop after the safety of the instructions is simulated and verified in the digital twin environment. The invention also provides a system for realizing the method. Through active prediction and simulation verification, the problems that in the prior art, sensing data are not accurate, a decision model is simple, regulation and control are lagged, and potential safety hazards exist are solved, the breeding environment can be accurately and safely optimized, and the intelligent level of breeding management is improved.

The invention is suitable for the technical field of molecular genetics, and provides an SNP molecular marker related to pig backfat thickness and application thereof. The invention studies and determines that the pig backfat thickness related molecular marker is located at the 95583333rd site (corresponding to the 701st basic group A / G mutation of SEQ ID NO: 1) of the No.18 chromosome of the international pig reference genome version 11.1, and verifies the influence effect of the molecular marker on the pig backfat thickness character. And when the basic group at the site is A, the pig has lower back fat thickness. On the basis, a molecular marker-assisted selection breeding technology for rapidly improving the backfat thickness character of the pig is established, the frequency can be increased generation by generation by optimizing the dominant allele (A), the backfat thickness of the breeding pig is remarkably reduced, the genetic improvement process of commercial pig herds containing landrace and large white pig blood lineage is accelerated, and the breeding efficiency is improved; the sales profit is increased for enterprises, the core competitiveness is improved, and the economic benefits of commercial pigs are improved.

The invention discloses an intelligent hog house based on a disease real-time monitoring and early warning system, which comprises a hog house, a ceiling is fixedly mounted on the upper surface of the hog house, an excrement collecting groove is formed in the lower surface in the hog house, a leakage net plate is fixedly mounted on the upper surface of the excrement collecting groove, a connecting cylinder is fixedly mounted on the lower surface of the leakage net plate, and a push shovel mechanism is rotatably mounted in the connecting cylinder; therefore, the push shovel mechanism can be pushed to slide in the manure collecting tank to push and shovel the gathered pig manure in a centralized manner. Through the design of the camera and the push shovel mechanism, a sensor and a data analysis method are utilized to construct a disease real-time monitoring and early warning system, omnibearing and real-time monitoring of pig herd health conditions is realized, disease hidden dangers are found in time, prevention and control measures are taken, disease loss is reduced, excrement in a pig house can be cleaned at regular time, and the hog house is protected. Breeding and spreading of bacteria and viruses in the pig house are reduced, the risk that pigs are infected with epidemic diseases is reduced, the overall health level of pig herds is improved, and breeding production safety is guaranteed.

The invention relates to the technical field of industrial big data and intelligent breeding, and particularly discloses a pig farm breeding management system and method based on industrial big data. The system comprises a multi-source heterogeneous data acquisition module, a data fusion and feature engineering module, a subclinical period anomaly detection module, a causal inference and root cause tracing module and a dynamic resource optimization scheduling module. High-order features are extracted through multi-source data fusion, a double-layer adaptive model is adopted to realize sub-clinical period anomaly detection, then root causes are deduced and positioned in combination with causality, and finally an accurate resource scheduling instruction is generated based on multi-objective optimization. According to the invention, extremely early warning and accurate intervention of the health state of the swinery can be realized, the disease outbreak risk is reduced, and the breeding benefit is improved.

The invention relates to a primer of a KLHL14 gene molecular marker related to pig weak litter number character and application of the primer, and belongs to the technical field of biology. The KLHL14 gene molecular marker is located at the 116519104 base of chromosome 6 of a pig reference genome Sscrofa11.1 version, the base is mutated into C or T, and the genotypes are C / C, C / T and T / T. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used as a genetic marker for pig breeding to breed pigs with less weak piglets; the method can efficiently and accurately detect the character of the number of the weak piglets of the pigs, improves the survival rate of healthy piglets of a swinery, and has an important value for breeding and production of the pigs.

The utility model discloses a movable pigsty for pig herd health management, and relates to the technical field of livestock breeding industry, the pigsty comprises a pigsty position adjusting mechanism and a protection mechanism, the pigsty position adjusting mechanism comprises a plurality of groups of second vertical pigsty bodies and first vertical pigsty bodies, and the first vertical pigsty bodies are sleeved in the second vertical pigsty bodies in a sliding mode; the protection mechanism comprises a first supporting rod and a second supporting rod which are arranged towards the inner area of the pigsty, the end of the first supporting rod is fixedly connected with the end of the second supporting rod, the first supporting rod is located above the second supporting rod, and the fixedly-connected position of the first supporting rod and the second supporting rod is rotationally connected to the first vertical fence. When the first vertical fence slides towards the outer side of the second vertical fence to a high-point protection position, the first supporting rod rotates until the highest end of the first supporting rod is higher than the upper end of the first vertical fence; when the first supporting rods are located at the initial positions, the highest ends of the first supporting rods are lower than the upper ends of the first vertical bars. The height of the pigsty can be adjusted, and pigs are effectively prevented from climbing over the pigsty by means of the first supporting rods.

This utility model relates to the field of door panel technology, specifically an infrared sensor-operated heat-insulating door for pigsties. Two second sliding grooves and positioning blocks are symmetrically arranged on the fixed plate. The positioning blocks are inserted into and slidably connected to the second sliding grooves. A second motor is provided at one end of the fixed plate, and a rotating shaft is provided at the output end of the second motor. Two second threaded rods with reverse thread structures are symmetrically arranged on the rotating shaft, and the two second threaded rods respectively pass through the two positioning blocks. By randomly detecting the piglets' body temperature through an infrared temperature sensor, piglets with abnormal body temperatures can be detected in a timely manner. Images captured by the camera provide farmers with more intuitive information to judge the health status of piglets, facilitating timely treatment measures, reducing the risk of piglet mortality due to disease, improving the overall health level of the pig herd, and ensuring farming efficiency.

The application provides a pig breeding and production integrated house group transfer planning and scheduling method and device, and relates to the technical field of intelligent breeding management. Through the fusion of health monitoring and reproductive performance double-dimensional data, the pigs are comprehensively rated and prioritized, so that the group transfer decision is no longer dependent on a single index or artificial experience, and high-value breeding pigs and pigs that need to be isolated can be accurately identified. In the process of combining pigs, the pig pathogen carrying state or health risk level is combined, and the same state priority merging principle is followed, so that the flow of different health state pigs is blocked, and biological safety control is strengthened. According to the loading scheme and the layout of the field, the target field is dynamically allocated and the transfer path is generated, the efficiency of the field is improved, and the stress of the pigs and the lag of the environmental control caused by the transfer confusion are reduced. Through scientific planning of the transfer process, the risk of disease outbreak and treatment cost are reduced, the smooth connection of each breeding link is ensured, and the survival rate of the pig group and the comprehensive breeding benefit are improved.

The invention provides a pAPN protein mutant, a substance for mutating pAPN protein and application of the pAPN protein mutant, which are characterized in that on the basis of wild pAPN protein, amino acid of the pAPN protein is mutated into 220 D into G, or / and 918 F into P, or / and 570 V into I, or / and 595 H into Y, and other amino acid residues are not changed, so that TGEV SD / L strain infection can be effectively inhibited; a target spot is provided for obtaining a pig herd which can effectively inhibit TGEV infection and does not affect the growth performance of piglets and the physiological activity function of TGEV.

The present application relates to the field of molecular markers and animal genetic breeding technology, and particularly relates to a SNP marker affecting pig purine base content and application thereof. Based on three test pig populations of American purebred Landrace, American purebred Large White and American purebred Duroc, the present application uses pig whole genome resequencing and GWAS analysis to research and determine 15 SNP markers significantly affecting pig purine base content. By selecting the advantageous alleles of the above SNP markers, the frequency of the advantageous alleles can be increased generation by generation, the corresponding purine content can be reduced or increased, the progress of pig genetic improvement can be accelerated, and the economic benefits of pig breeding can be effectively improved.

The invention discloses a clostridium butyricum preparation for preventing and treating porcine reproductive and respiratory syndrome and application of the clostridium butyricum preparation, and belongs to the technical field of microbial fermentation feed. According to the invention, the clostridium butyricum with the preservation number of CCTCC NO: M 20231636 is subjected to activation culture, seed culture and high-density fermentation culture to prepare the clostridium butyricum preparation, and the effect of the clostridium butyricum preparation in prevention of PRRSV infection is developed. Experimental results show that when the clostridium butyricum preparation is added into a pig complete feed, the positive infection rate of the porcine reproductive and respiratory syndrome of a pig herd can be effectively reduced, the death number of the pig herd is remarkably reduced, and the clostridium butyricum preparation has outstanding prevention and treatment effects. The clostridium butyricum preparation provided by the invention is free from drug resistance and toxic and side effect risks, has the advantages of low cost, high safety, environment friendliness and the like, and has great significance in benefit improvement of the live pig breeding industry.

The invention discloses an automatic atomization method and atomization equipment, and relates to the technical field of livestock breeding, the automatic atomization method and atomization equipment for large-scale breeding comprises the steps that a sound monitor is installed in a breeding house, cough information of a pig herd is collected through the sound monitor, and when the sound monitor collects the cough information of the pig herd, the sound monitor sends the cough information of the pig herd to the atomization equipment; the method comprises the following steps: automatically starting atomization equipment, when the atomization equipment is started for a preset time, automatically stopping working, and repeating the steps until the sound monitor does not detect the cough information of the pig herd, the atomization equipment comprises a rack, an air supply fan and a control box, an atomization box body and a liquid storage box are mounted on the rack, an atomization plate is mounted in the atomization box body, and the liquid storage box is mounted in the liquid storage box; the system can monitor the interior of a breeding house for 24 hours, especially effectively monitors cough of swinery at night, timely discovers diseases existing in the swinery in the early stage, automatically carries out atomization medication on the swinery, greatly reduces the severity of the diseases of the swinery, and effectively ensures the growth health of the swinery.

The present application relates to a method for identifying a sex antagonistic hotspot region on a pig autosomal chromosome and application thereof. The method provided by the present application comprises the following specific steps: constructing a hybrid population F0, F1, F2 three generations, genome sequencing and SNP identification, transmission tracking of genetic material in the hybrid population pedigree, and identifying a sex antagonistic hotspot region. The method of the present application identifies that the Chr3:121M window is a sex ratio directional regulation molecular marker that is beneficial to the generation of male offspring in the Large White / Mini pig genotype individual. According to the method of the present application, the pig population sex can be predicted and regulated, and by screening and optimal mating of the genotype of the parent before mating, a plurality of generations is obtained by continuous cross without introducing exogenous blood relationship, so that the maximum value of the proportion of the genotype beneficial to the male or female is achieved under the condition that all genotypes of each generation are retained at the population level, and then the dynamic regulation of the sex ratio of the offspring pig population is achieved.

The invention belongs to the technical field of reuse water utilization of farms, and particularly discloses a reuse water utilization system and method for spraying of a breeding house, and the system comprises a water regeneration device which is connected with a first water storage tank through a regeneration water outlet pipe; the first water storage tank is used for temporarily storing reclaimed water, a submersible filter and a throw-in type liquid level meter are arranged in the first water storage tank, the first water storage tank is connected with the water purification tank through a filtered water outlet pipe, and the filtered water outlet pipe is further connected with a first dosing device; a first water quality detection device is arranged in the water purification tank, and water in the water purification tank is connected with the second water storage tank through a purified water outlet pipe; and the second water storage pond is connected with a second dosing device, a second water quality detection device is arranged in the second water storage pond, and the second water storage pond is externally connected with the breeding house spraying pipeline through a clear water outlet pipe. Normal operation of the spraying equipment can be guaranteed, no odor exists, reuse water is safe to use, and the health and the death rate of pig herds cannot be affected.

The application provides a method and system for realizing closed-loop management of pig raising by using an online operation analysis system, comprising: setting production mode parameters and pig flow operation mode; determining data sources according to the production mode parameters and the pig flow operation mode; performing forward calculation when the data sources are existing inventory; performing rolling prediction of mating when the data sources are future plans; generating future daily pig population structure according to the forward calculation and the rolling prediction of mating; calculating predicted total cost and estimated cost per head of each month in the future according to calculated feed cost, fixed cost allocation cost and variable cost; calculating predicted total profit according to the estimated cost per head and predicted output; comparing the predicted total profit with the actual total profit; realizing high-precision future deduction based on the current inventory and the production parameters; establishing a dynamic linkage mechanism of production indicators and cost and profit; realizing digital closed loop from target setting, process early warning to operation review.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP (Single Nucleotide Polymorphism) marker capable of influencing the content of IMP or GMP (Good Manufacturing Practice) of pigs and application of the SNP marker. According to the invention, based on three test swinery of American-series purebred landrace, American-series purebred large white pig and American-series purebred Duroc, pig whole genome re-sequencing and GWAS analysis are adopted, 15 SNP markers related to the content of IMP or GMP are researched and determined, and by optimizing the dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the content of IMP or GMP can be increased, and the number of SNP markers related to the content of IMP or GMP can be increased. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention belongs to the field of biology, and discloses a PDCoV recombinant RBD protein and an IgA Elisa kit thereof, and the amino acid sequence of the recombinant RBD protein is as shown in SEQ ID NO. 1. The recombinant RBD protein is used for an indirect ELISA kit, can cover IgA response in the early stage of infection, can accurately recognize a PDCoV specific IgA antibody in a breast milk sample, and cannot cause false positive due to infection of other common pathogens in a pig herd; the indirect ELISA kit is good in stability (the intra-batch variation coefficient is 1.848%-6.062%, and the inter-batch variation coefficient is 4.504%-7.790%), the coincidence rate of the indirect ELISA kit and an immunofluorescence experiment reaches 93.26%, the repeatability and the coincidence rate are high, and the indirect ELISA kit has the advantages of simplicity and convenience in operation, short time consumption and large detection flux of an ELISA method, and is suitable for large-scale screening of clinical pig milk samples.

This invention belongs to the field of animal feed technology and discloses the application of glucuronolactone in the preparation of feed that alleviates spleen damage caused by the combined effects of PRRSV and DON. This invention discovers that glucuronolactone can effectively alleviate spleen damage in piglets caused by co-exposure to PRRSV and DON. It exerts its protective effect by activating the Nrf2 antioxidant signaling pathway, improving spleen tissue structure, alleviating oxidative stress, inhibiting inflammatory responses, and inhibiting apoptosis. This invention also provides a feed containing the aforementioned effective amount of glucuronolactone, with the basic feed tailored to the nutritional needs of piglets. This invention fills the technological gap in effective intervention methods for spleen damage under PRRSV and DON co-exposure. The glucuronolactone addition process is simple, the effect is significant, and it is suitable for large-scale feed production. It can effectively solve the problem of immunosuppression in pig herds caused by the combined harm of PRRSV and DON in the pig industry, and has important practical value.

A method and system for counting pigs in a hog house are provided. The system includes a computing device and an imaging device. The computing device has a processor and a storage device having computer executable code stored therein. The computer executable code is configured to: receive images captured from one end of the house to the other end of the house; detect key points in the images using a neural network; identify pig skeletons by associating a plurality of related key points; track the skeletons in the images to obtain trajectories; divide each image into active zones and deactive zones; assign a spatial value of 0 to the skeletons in the active zones and a spatial value of 1 to the skeletons in the deactive zones; sum a first order difference of the spatial values for each trajectory to obtain a trajectory count; and add the trajectory counts to obtain a pig count.

This utility model discloses a quantitative structure for a feeding device, relating to the technical field of quantitative structures for feeding devices. It includes a feeding hopper, a support frame, a feeding tray, a feeding box, a pusher plate, a quantitative trough, a discharge pipe, a control handle, and a positioning shell. The surface of the feeding hopper is fixedly connected to the surface of the support frame, the bottom of the support frame is fixedly connected to the surface of the feeding tray, the top of the feeding box is fixedly connected to the bottom of the feeding hopper, the top of the discharge pipe is fixedly connected to the bottom of the feeding box, and the pusher plate is movably connected to the inner cavity of the feeding box. This utility model, by setting a positioning device and a moving plate device, solves the problem that the quantitative function of pig feeding devices is a key design element for ensuring efficiency and effectiveness in modern pig farming. Its core function is to achieve scientific feeding by precisely controlling the amount of feed delivered. If the feeding device cannot quantitatively dispense feed, it can easily lead to serious feed waste, soaring farming costs, and uneven growth in the pig herd.

The invention discloses a pig raising pig house, relates to the technical field of pig raising, and solves the problems that pigs possibly directly cross rotating rollers and cannot be driven by the rotating rollers to walk back and forth in the pig house, so that the amount of exercise of the pigs is relatively small, and the amount of exercise of the pigs in the set time cannot be calculated; the hog house comprises a hog house body, a supporting frame is installed in the hog house body, the hog house further comprises a pushing and driving mechanism used for pushing the live pigs to walk left and right in the hog house body in a reciprocating mode, and the pushing and driving mechanism is installed on the lower side of the supporting frame; the anti-collision assembly is used for preventing the pushing mechanism from descending to collide with the live pigs; according to the pig house, by installing the pushing and driving mechanisms, the pig herd can be pushed and driven to move left and right on the two sides of the pig herd, so that live pigs can walk or run left and right in the pig house body in a reciprocating mode in the specified movement time period, the amount of movement of the live pigs in the movement time period is large, and the movement duration of the live pigs can be conveniently controlled.

The invention discloses a porcine reproductive and respiratory syndrome virus typing quadruple fluorescent quantitative RT-PCR (reverse transcription-polymerase chain reaction) detection composition and a kit thereof. The detection composition is used for simultaneously detecting four viruses, namely PRRSV-2 (porcine reproductive and respiratory syndrome virus 2), NADC30-like PRRSV (porcine reproductive and respiratory syndrome virus), HP-PRRSV (porcine reproductive and respiratory syndrome virus) and NADC34-like PRRSV; the kit obtained on the basis of the detection composition has good sensitivity, specificity and repeatability, nucleic acids of PRRSV-2, NADC30-like, HP-PRRSV and NADC34-like PRRSV strains can be effectively distinguished, the virus types can be accurately and rapidly identified by analyzing fluorescent quantitative results, the detection period is greatly shortened, and the kit has the advantages of being easy to operate, high in sensitivity, rapid in detection and the like, and has a good application prospect in the detection of PRRSV-2, NADC30-like, HP-PRRSV and NADC34-like PRRSV strains. And a new tool is provided for monitoring the propagation of the PRRSV in the swinery.

This invention relates to the fields of molecular markers and animal genetic breeding technology, and particularly to a SNP marker affecting the total flavor nucleotide content in pigs and its application. Based on three experimental pig populations—purebred American Landrace, purebred American Large White, and purebred American Duroc—this invention employs whole-genome resequencing and GWAS analysis to study and identify nine SNP markers associated with total flavor nucleotides. By optimizing the dominant alleles of these SNPs, the frequency of dominant alleles can be increased generation by generation, thereby increasing the total flavor nucleotide content, improving pork quality, accelerating the progress of pig genetic improvement, and effectively improving the economic benefits of pig breeding.