The invention discloses a preparation method and application of an acidic degradable
hybrid nano composite for treating
osteosarcoma. The preparation method comprises the following steps: 1, preparing
hybrid silicon dioxide / hydroxyapatite nano particles MH; 2, dispersing MH into absolute ethyl
alcohol, adding
ultrapure water,
ammonia water and 3-aminopropyltriethoxysilane, heating and stirring to react, centrifuging and washing to obtain MH-NH2; 3, dispersing the MH-NH2 into
ultrapure water, adding DATS, stirring and reacting at
room temperature, centrifuging and washing to obtain MHD; and 4, dispersing the MHD into
ultrapure water, adding a
carboxymethyl chitosan solution under a stirring condition, reacting, centrifuging and washing to obtain the nano-composite C-coated MHD. According to the nano-composite disclosed by the invention, the
calcium overload performance of the
hybrid silicon dioxide / hydroxyapatite nano-particles is combined with H2S damaged
DNA released by
hydrophobic drug diallyl trisulfide, so that the death of
cancer cells is accelerated, and the purpose of treating
osteosarcoma is achieved.