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Application of diallyl trisulfide to preparation of preparation for inhibiting pseudomonas aeruginosa secretion systems

A technology of diallyl trisulfide and Pseudomonas aeruginosa, which is applied in the direction of antibacterial drugs, organic active ingredients, and medical preparations containing active ingredients, and can solve the problems of easy colonization, multi-drug resistance, and pseudomonas aeruginosa bacterial infection etc.

Inactive Publication Date: 2021-08-06
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Under normal circumstances, Pseudomonas aeruginosa will not infect human tissues, but Pseudomonas aeruginosa infection is extremely prone to occur when the human body has low immunity, severe trauma or surgical treatment, cystic pulmonary fibrosis, and malignant tumor chemotherapy and radiotherapy
The bacterium has the characteristics of multi-drug resistance, easy mutation, and easy colonization, which brings great challenges to clinical treatment.

Method used

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  • Application of diallyl trisulfide to preparation of preparation for inhibiting pseudomonas aeruginosa secretion systems
  • Application of diallyl trisulfide to preparation of preparation for inhibiting pseudomonas aeruginosa secretion systems
  • Application of diallyl trisulfide to preparation of preparation for inhibiting pseudomonas aeruginosa secretion systems

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Experimental program
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Embodiment 1

[0018] Preparation of Pseudomonas aeruginosa (Pseudomonas aeruginosa) PAO1 suspension: sample Pseudomonas aeruginosa PAO1 culture solution in exponential growth phase, centrifuge, wash once with PBS buffer, resuspend in PBS, and dilute the bacterial concentration to 10 8 CFU / mL to obtain Pseudomonas aeruginosa PAO1 bacterial suspension.

[0019] 1. Effect of diallyl trisulfide on the growth of Pseudomonas aeruginosa

[0020] Add LB medium and diallyl trisulfide to the test tube respectively, and insert Pseudomonas aeruginosa PAO1 bacterial suspension in the exponential growth phase, the total volume is 10mL, so that the bacterial concentration of Pseudomonas aeruginosa PAO1 Both are 10 6 The concentrations of CFU / mL and diallyl trisulfide were 0 (control), 0.16 μl / mL, 0.63 μl / mL, 1.25 μl / mL, 2.5 μl / mL and 5.0 μl / mL, respectively. Several experimental groups were sampled and added to a honeycomb culture plate dedicated to an automatic growth curve analyzer (Bioscreen C), and...

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Abstract

The invention discloses application of diallyl trisulfide to preparation of a preparation for inhibiting pseudomonas aeruginosa secretion systems. The invention finds that the diallyl trisulfide slightly inhibits the growth of a pseudomonas aeruginosa PAO1 strain, but can obviously inhibit the expression of key genes in II-type and VI-type secretion systems of the pseudomonas aeruginosa PAO1 strain, and inhibit the function of the secretion systems of the pseudomonas aeruginosa PAO1 strain and the secretion of virulence factors of the pseudomonas aeruginosa PAO1 strain. Therefore, the diallyl trisulfide can be applied to preparation of the preparation for inhibiting the pseudomonas aeruginosa secretion systems for controlling the toxicity and pathogenicity of the pseudomonas aeruginosa.

Description

Technical field: [0001] The invention belongs to the technical field of harmful microorganism prevention and control, and specifically relates to the application of diallyl trisulfide in the preparation of preparations for inhibiting the secretion system of Pseudomonas aeruginosa. Background technique: [0002] Pseudomonas aeruginosa is a common Gram-negative nosocomial opportunistic pathogen that can cause fatal infectious diseases. It widely exists in nature, soil, air, and human skin, intestinal tract, respiratory tract, and mucous membranes, and can be transmitted through various routes. Under normal circumstances, Pseudomonas aeruginosa does not infect human tissues, but Pseudomonas aeruginosa infection is extremely prone to occur when human immunity is low, severe trauma or surgical treatment, cystic pulmonary fibrosis, and malignant tumor chemotherapy and radiotherapy . The bacterium has the characteristics of multi-drug resistance, easy mutation, and easy colonizat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/255A61P31/04
CPCA61K31/255A61P31/04
Inventor 李文茹张志清谢小保刘静霞施庆珊
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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