Application of diallyl trisulfide to preparation of preparation for inhibiting pseudomonas aeruginosa secretion systems
A technology of diallyl trisulfide and Pseudomonas aeruginosa, which is applied in the direction of antibacterial drugs, organic active ingredients, and medical preparations containing active ingredients, and can solve the problems of easy colonization, multi-drug resistance, and pseudomonas aeruginosa bacterial infection etc.
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[0018] Preparation of Pseudomonas aeruginosa (Pseudomonas aeruginosa) PAO1 suspension: sample Pseudomonas aeruginosa PAO1 culture solution in exponential growth phase, centrifuge, wash once with PBS buffer, resuspend in PBS, and dilute the bacterial concentration to 10 8 CFU / mL to obtain Pseudomonas aeruginosa PAO1 bacterial suspension.
[0019] 1. Effect of diallyl trisulfide on the growth of Pseudomonas aeruginosa
[0020] Add LB medium and diallyl trisulfide to the test tube respectively, and insert Pseudomonas aeruginosa PAO1 bacterial suspension in the exponential growth phase, the total volume is 10mL, so that the bacterial concentration of Pseudomonas aeruginosa PAO1 Both are 10 6 The concentrations of CFU / mL and diallyl trisulfide were 0 (control), 0.16 μl / mL, 0.63 μl / mL, 1.25 μl / mL, 2.5 μl / mL and 5.0 μl / mL, respectively. Several experimental groups were sampled and added to a honeycomb culture plate dedicated to an automatic growth curve analyzer (Bioscreen C), and...
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