Molecular marking method for predication and identification of sheep multiple ovulation trait and molecular marker primers

Abstract

The invention discloses a molecular marking method for predication and identification of a sheep multiple ovulation trait and molecular marker primers, and discloses the molecular marking method. With utilization of a sheep FSHR gene 5' control region c.-365C>T mutation (C-365T) mutation site, the molecular marker primers are designed to be FSHRPF and FSHRPR. The method comprises the steps: firstly, amplifying a sheep FSHR gene 5' control region section by the primers; secondly, carrying out enzyme digestion detection on the C-365T mutation site in the PCR amplified fragment by using Sau3AI; thirdly, carrying out genotype determination; and fourthly, constructing a linear model, and carrying out correlation analysis, wherein a BB genotype donor has higher ovulation number in a process of multiple ovulation, and has higher multiple ovulation efficiency. The method is simple to operate, low in detection cost, high in precision, and suitable for automatic detection. The relevance of the genotype information of the FSHR gene 5' control region section with the sheep multiple ovulation trait is utilized.

Description

technical field [0001] The invention belongs to the technical field of animal genetic engineering, and in particular relates to a method for predicting and identifying superovulation traits of sheep by using molecular markers. Background technique [0002] The multiple ovulation (MO) technology is a biological high-tech that has developed rapidly in the world in the past 30 years. In the developed countries of animal husbandry, the super ovulation technology of sheep has been gradually transferred from the laboratory to the end of the last century. application stage. The application of this technology enables people to get rid of the restrictions on the reproductive research and application of female animals in the past, and begin to excavate and utilize the genetic potential of excellent female animals. Using this technology, one ewe can obtain With more offspring, it can breed high-quality sheep several times and dozens of times faster than traditional methods, which will...

AI Technical Summary

Problems solved by technology

Unstable superovulation effect has become the primary factor restricting its further popularization and application, even if the breed, age, nutritional and physiological condition of the donor ewes, the source and dosage of hormones, the season and procedure of superovulation are all the same Under these conditions, the effect of superovulation will still vary to a large extent. The number of retrieved eggs varies greatly among different individuals, and the coefficient of variation is about 40%, which leads to a significant decline in the efficiency of superovulation.

Images