Method and kit for detecting Chinese holstein cattle lactoprotein ration property

A Holstein cow milk protein technology, applied in biochemical equipment and methods, microbial measurement/testing, etc., can solve the problem of affecting GH binding ability and signal transduction process, affecting the structure and function of expressed proteins, and affecting the normal function of GH problems such as improving milk production performance, speeding up the breeding process, and achieving reliable results

Inactive Publication Date: 2008-04-30
CHINA AGRI UNIV
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the variation of the GHR gene structure may affect the structure and function of its expressed protein, thereby affecting the binding ability and signal transduction process of GH, th...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and kit for detecting Chinese holstein cattle lactoprotein ration property
  • Method and kit for detecting Chinese holstein cattle lactoprotein ration property
  • Method and kit for detecting Chinese holstein cattle lactoprotein ration property

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Detection of Chinese Holstein milk protein rate traits

[0028] 1. Selection of experimental materials

[0029] The Chinese Holstein cattle in this study came from 13 cattle farms of Beijing Sanyuan Group Lvhe Dairy Cow Breeding Center. A total of 15 bulls were selected for offspring, and the average number of daughters per bull was 76 (ranging from 12 to 150). A total of 1145 heads (Table 1).

[0030] Table 1 Number of bulls and their daughters

[0031] bull number

number of daughters

bull number e

number of daughters

1

18

9

106

2

20

10

52

3

12

11

121

4

118

12

14

5

117

13

148

6

150

14

104

7

67

15

58

8

40

Total

1145

[0032] 2. Detection of the genotype of Chinese Holstein cattle

[0033] 1. Extractio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method and a kit for detecting Chinese Holstein milk protein rate traits. The method to detect the milk protein rate of Chinese Holstein cows is to detect whether the deoxyribonucleotide of Chinese Holstein cattle from GenBank AccessionNo. , determine the genotype of the Chinese Holstein cattle, and then determine the milk protein rate trait by genotype. The kit of the present invention includes primers for PCR amplification of a genomic fragment containing nucleotides from the 873rd position from the 5' end (ie, the 279th position of GHR exon 8) from GenBank Accession No. NM_176608, restriction nucleic acid Endonuclease SSpI, PCR reagents other than the primers and restriction endonuclease buffer. The method and the kit of the invention are easy to operate, fast and sensitive, and the results detected by the method and the kit are reliable, stable and accurate. Therefore, the method and the kit of the present invention will have broad application prospects in the field of animal breeding.

Description

technical field [0001] The invention relates to a method and a kit for detecting the traits of Chinese Holstein milk protein rate. Background technique [0002] At present, the average yield per unit area of ​​dairy cows in major countries in the world can be divided into three levels: the United States, Canada, Japan, Israel and the European Union belong to the first level. The average yield per unit area of ​​dairy cows is above 6,000 kg. These countries mainly use intensive captive breeding; Australia, New Zealand, and Argentina belong to the second level. The average yield per unit of dairy cows is about 4,000 kilograms. These countries have large grasslands, and dairy cows are mainly raised on grazing, which has the advantage of low production costs; China, Brazil, Mexico and other countries belong to the third level. At different levels, the average yield per unit of dairy cows is less than 2,000 kg. It can be seen that the average yield level, breed structure and pro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
Inventor 张沅孙东晓马妍
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap