MUC4 molecular marker capable of identifying western pig breed weaned piglet F4 diarrhoea susceptibility/resistance and use thereof
A technology for weaned piglets and molecular markers, applied in the fields of application, botany equipment and methods, biochemical equipment and methods, etc., can solve problems such as sales revenue decline, feeding cost increase, affecting growth and development, etc.
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[0516] The present invention will be described in further detail below in conjunction with the embodiments and with reference to the accompanying drawings.
[0517] Select individuals from the core group of breeding pigs, use the above-mentioned PCR-RFLP method to identify the genotypes of the four polymorphic sites of MUC4, construct haplotypes, select favorable haplotypes for individual breeding, and improve the resistance of the population after subculture selection. Piglet diarrheal disease performance.
[0518] Example:
[0519] The self-constructed pig resource family with white Duroc and Chinese Erhualian pigs as ancestors and used for functional gene mapping was used as experimental animals. 347 heads of the second generation of resource families (F2 generation) and 108 heads of the third generation (F 3 Generation) boars were raised until they were 240 days old and slaughtered. The jejunum part of the small intestine was collected to extract the brush border of smal...
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