MUC4 molecular marker capable of identifying western pig breed weaned piglet F4 diarrhoea susceptibility/resistance and use thereof

A technology for weaned piglets and molecular markers, applied in the fields of application, botany equipment and methods, biochemical equipment and methods, etc., can solve problems such as sales revenue decline, feeding cost increase, affecting growth and development, etc.

Active Publication Date: 2009-02-25
JIANGXI AGRICULTURAL UNIVERSITY
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Problems solved by technology

Even if the individual recovers from the disease, it will seriously affect the growth and developmen...

Method used

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  • MUC4 molecular marker capable of identifying western pig breed weaned piglet F4 diarrhoea susceptibility/resistance and use thereof
  • MUC4 molecular marker capable of identifying western pig breed weaned piglet F4 diarrhoea susceptibility/resistance and use thereof
  • MUC4 molecular marker capable of identifying western pig breed weaned piglet F4 diarrhoea susceptibility/resistance and use thereof

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Embodiment Construction

[0516] The present invention will be described in further detail below in conjunction with the embodiments and with reference to the accompanying drawings.

[0517] Select individuals from the core group of breeding pigs, use the above-mentioned PCR-RFLP method to identify the genotypes of the four polymorphic sites of MUC4, construct haplotypes, select favorable haplotypes for individual breeding, and improve the resistance of the population after subculture selection. Piglet diarrheal disease performance.

[0518] Example:

[0519] The self-constructed pig resource family with white Duroc and Chinese Erhualian pigs as ancestors and used for functional gene mapping was used as experimental animals. 347 heads of the second generation of resource families (F2 generation) and 108 heads of the third generation (F 3 Generation) boars were raised until they were 240 days old and slaughtered. The jejunum part of the small intestine was collected to extract the brush border of smal...

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Abstract

The invention discloses a MUC4 molecular marker capable of identifying the susceptibility/resistibility to diarrhea of weaned piglet F4 of the western pigs and applicable to the genetic improvement of boars, and the application of the MUC4 molecular marker in breeding anti-diarrhea disease traits of the improved boars. Firstly, an identification of MUC4 polymorphic sites is processed, wherein, g.13383C is greater than T, g.15580A is greater than G, g.21208A is greater than G, and g.26002A is greater than C. Secondly, genotyping determination is applied to each of the polymorphic sites by using the PCR-RELP method. Finally, a haplotype is constructed. The invention detects the four polymorphic sites by the modern molecular biology technique, constructs the haplotype, and selects a favorable haplotype to reserve seeds for individuals by using marker assisted selection (MAS), thus being capable of significantly improving the ability to resist the diarrhea disease of the species weaned piglets and greatly reducing death rate of the weaned piglets.

Description

technical field [0001] The invention relates to a MUC4 molecular marker which can identify the susceptibility / resistance to diarrhea of ​​F4 weaned piglets of western pig breeds and can be used for genetic improvement of breeding pigs and the application of the MUC4 molecular marker in breeding and improving the diarrhea resistance traits of breeding pigs. Background technique [0002] Piglet diarrhea is a common infectious disease in pig production, which has caused huge economic losses to the world's pig industry. A large-scale inter-field survey of the Danish pig industry showed that 6%-7% of newborn piglets suffered from diarrheal disease, causing the mortality rate of piglets before weaning to reach 2.7%, accounting for 11.9% of the entire mortality rate during this period. Even the recovered individual will seriously affect the growth and development, and some will form dead pigs, resulting in increased feeding costs and decreased sales revenue. [0003] Enterotoxigen...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/12
Inventor 黄路生任军晏学明黄翔欧阳婧曾维宏
Owner JIANGXI AGRICULTURAL UNIVERSITY
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