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Assisted selection marker for abdominal fat weight and carcass weight of goose bred for liver, and method for assisting selection with molecular marker

A molecular marker-assisted and assisted selection technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., to improve breeding efficiency, reduce workload, and improve germplasm

Active Publication Date: 2018-12-18
YANGZHOU UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem of marker-assisted selection of goose belly fat weight and carcass weight for liver, and provides an auxiliary selection marker for goose belly fat weight and carcass weight for liver and a method for using molecular marker-assisted selection

Method used

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  • Assisted selection marker for abdominal fat weight and carcass weight of goose bred for liver, and method for assisting selection with molecular marker
  • Assisted selection marker for abdominal fat weight and carcass weight of goose bred for liver, and method for assisting selection with molecular marker
  • Assisted selection marker for abdominal fat weight and carcass weight of goose bred for liver, and method for assisting selection with molecular marker

Examples

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Embodiment Construction

[0023] Take a batch of 66 Langde geese as example to set forth the present invention below:

[0024] 1) Select the individuals of Lande geese to be tested: 66 healthy individuals with uniform body weight were selected from a group of Lande geese.

[0025] 2) Blood extraction and DNA extraction: 0.1 mL of blood was extracted from the wing vein of the individual to be tested. Using a DNA extraction kit, extract the DNA template.

[0026] 3) Polymerase chain reaction and product detection: Use the extracted DNA as the template in PCR and the provided PCR kit (see attached table 1 for the list of reagents included) to carry out PCR (see attached table 2 for the reaction system, reaction See attached table 3 for details). Take 5 μL of the product and carry out 1.5% agarose gel electrophoresis, if there is a single band with a size of 912bp (see attached figure 1 ), send the PCR product to the sequencing company for detection.

[0027] 4) Sequencing and determining the genotype:...

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Abstract

The invention specifically relates to an assisted selection marker for the abdominal fat weight and carcass weight of a goose bred for the liver, belonging to the technical field of poultry breeding.The assisted selection marker has a SNP site which is located at the downstream position 807 bp of the initiator codon of the MC5R gene. The invention also discloses a method for assisted selection ofthe abdominal fat weight and carcass weight characters of the goose bred for the live by using the molecular marker. The method comprises the following steps: selecting to-be-detected Landes goose individuals; drawing blood and extracting DNAs; performing a polymerase chain reaction (PCR) by using a provided kit and detecting a product; delivering the PCR product to a sequencing company for sequencing and genotype determination; and selecting qualified individuals according to the genotypes and keeping the selected qualified individuals as breeding geese. The marker and the method of the invention can overcome the disadvantages that a slaughtering determination method leads to high cost and great workload and excellent individuals cannot be kept as breeding geese, and can be used for large-scale breeding geese selection and speed up breeding process.

Description

technical field [0001] The invention belongs to the technical field of poultry breeding, and in particular relates to a method of using candidate gene nucleotide polymorphisms to assist in the selection of abdominal fat weight and carcass weight of fat geese to breed high-quality fat geese. It can be used by fat gras goose breeding enterprises and research units. Background technique [0002] The liver production performance of geese varies greatly among different breeds and within the same breed, indicating that genetic factors are important influencing factors. Therefore, the production performance of fat gras goose can be improved by means of genetic breeding, but the effect of phenotypic selection and slaughter test on the improvement of liver performance is not good or the cost is high. In contrast, marker-assisted selection can not only avoid the high cost and indirect selection problems caused by slaughtering live poultry, but also significantly improve the accuracy ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 刘龙耿拓宇刘同君赵盼龚道清
Owner YANGZHOU UNIV
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