37results about How to "Achieve early selection" patented technology

The invention discloses a molecular marker and specific primers for assisting in test of wilt disease resistance in brassica oleracea and use thereof. The invention provides a reagent for assisting in the test of wilt disease resistance in brassica oleracea and / or assisting in screening brassica oleracea with wilt disease resistance, which is a specific primer pair formed by nucleotides represented by a sequence 2 and a sequence 3 in a sequence table. The invention also provides a specific gene fragment which is at a genetic distance about 2.87cm to the wilt disease resistance gene in brassica oleracea and is formed by nucleotides represented by a sequence 1 in a sequence table. The result of the identification of wilt disease resistance in brassica oleracea and / or screening of the brassica oleracea with wilt disease resistance with assistance from the reagent (primer pair) or sequence characterized amplified region (SCAR) marker, which are provided by the invention, is 97 percent consistent with that of field identification. When used in breeding, the reagent, molecular marker or method has the advantages of accuracy, quickness, capability of realizing early breeding and the like and has a bright application prospect.

The invention discloses a molecular marker relevant to the reproductive performance of chicken and application of the molecular marker in breeding, belonging to the field of molecular genetics. The nucleotide sequence of the molecular marker is shown in SEQ ID No.1; a C->T base substitution exists at the 315th bp (base pair) of the sequence, thereby leading polymorphism of SspI-RFLP. The genome DNA of chicken is taken as a template, and primers shown in SEQ IDNo.2-3 are used for amplifying to obtain a PCR amplification product of 556bp; the PCR product is detected by using 1.5 percent agarose gel electrophoresis after being digested by an SspI restriction enzyme to obtain three banding patterns of CC, CT and TT; in local varieties with small egg weights and poor egg laying performance, CC genetype individuals are for reserved. A method for detecting the molecular marker relevant to the reproductive performance of chicken is simple and rapid and is not influenced by the environment, and early strain selection can be realized.

The invention relates to a molecular marker related to laying performance of laying ducks and its application in breeding, belonging to the technical field of molecular markers. The nucleotide sequence of the molecular marker is shown in SEQ ID NO.1. In the sequence There is a C>T mutation at the 108th position of the duck, which is significantly correlated with the age at first laying, egg production at 34 weeks and egg production at 72 weeks, and the age at first laying of the TT genotype is significantly Lower than the CC and CT genotypes, the 34-week-old egg production of the TT genotype was significantly higher than that of the CC genotype, and the 72-week-old egg production of the TT genotype was significantly higher than that of the CC and CT genotypes. Sequence type, select TT genotype duck. The invention realizes the early selection and breeding of duck egg-laying traits, and provides powerful assistance for the breeding work of egg-type ducks.

The invention relates to a molecular marking method, in particular to a molecular marking method for predicting and identifying the length of sheep wool. The method comprises the steps as follows: 1), extracting sheep genome DNA, designing a primer, performing PCR (polymerase chain reaction) amplification, performing enzyme digestion, and obtaining an enzyme digestion product; 2), subjecting the enzyme digestion product to electrophoretic separation, and judging genotypes according to an electrophoretic separation result; 3), performing association analysis and estimating the least square mean value of characters, and obtaining a result that the length of wool of a GG genotype individual is remarkably higher than that of wool of AA genotype and AG genotype individuals in the three genotypes; and 4), constructing wool length character breeding population taking the GG genotype individual as the primary so that the length of the sheep wool is predicated and identified. According to the method, the operation is simple, the cost is low, the accuracy is high, and automatic detection can be performed. Selective breeding of the sheep wool length is performed with the molecular marking method, so that a genetic breeding process of the length characters of the sheep wool can be accelerated, breeding sheep can be early selected, the sheep can be selected and left after birth, and a sheep breeding process is accelerated.

The invention relates to a molecular marking method for two mutation sites in a chicken PTHLH gene 5' regulatory region and application thereof in chicken breeding. Two mutation sites (namely the -1827 (A>G) site and the -165(C>T) site) exist in the chicken PTHLH gene 5' regulatory region, SHEsis online software is used for linkage disequilibrium analysis, and the result shows that the two sites in recessive white plymouth rock are in a complete linkage state. The two sites are related with the first-egg age and egg laying quantity at the 32th week, the first-egg age of AC / GT gene type is remarkably earlier than that of the GT / GT type and the AC / AC type, and meanwhile the egg laying quantity at the 32th week is remarkably larger than that of the other two types. The method is simple, fast and beneficial for breeding chicken variety with the early first-egg age and the large egg laying quantity and provides favorable help for marker-assisted breeding work.

The invention relates to the field of molecular genetics, in particular to a molecular marker method for two mutation sites of a chicken MMP13 gene 5' control region and application of the molecular marker method in chicken breeding. It is found by the inventor that six mutation sites exist in the chicken MMP13 5' control region, namely, -1719 (T>C), -1661 (C>A), -1356 (G>A), -1128 (A>G), -1094 (C>A) and -1079 (T>C); linkage disequilibrium analysis is performed through the SHEsis online software, and it is found through the result that the sites -1719 and -1661, the sites -1356 and -1128 and the sites -1094 and -1079 are respectively in a completely-linked state in White Recessive Rock. The method is easy, convenient and fast to implement, beneficial for breeding chicken breeds laying eggs early at a high yield and capable of providing favorable help for the marker-assisted breeding work.

The invention belongs to the technical field of molecular assisted breeding, in particular to a PCR primer set for identifying or screening cabbage hybrid lethal parent type 2 and its application. The PCR primer set includes primers KBoHL2-X, KBoHL2-Y, and KBoHL2-Common. The present invention detects whether the genotype at positions 46, 333, and 244 of the C04 chromosome of the cabbage to be tested is T / T or C / C, and determines whether the cabbage has the characteristics of hybrid lethal parent type 2 according to the genotype of the cabbage to be tested: T / T genotype of the cabbage to be tested It is the hybrid lethal parent type 2, and the tested cabbage of the C / C genotype is not the hybrid lethal parent type 2. Using the PCR primers, kit or method of the present invention for assisted breeding has the advantages of simple operation, strong specificity, good stability, and early breeding can be realized, and the coincidence rate of identification results and field phenotypes reaches 100%. It has great application prospect.