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Molecular marker relevant to laying duck egg laying performance and application thereof in breeding

A technique for molecular markers and egg production performance, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc.

Active Publication Date: 2018-09-18
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the MTNR1C gene in laying ducks, so it cannot be applied to duck breeding

Method used

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  • Molecular marker relevant to laying duck egg laying performance and application thereof in breeding
  • Molecular marker relevant to laying duck egg laying performance and application thereof in breeding
  • Molecular marker relevant to laying duck egg laying performance and application thereof in breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Cloning, sequencing and polymorphism analysis of duck MTNR1C gene

[0019] The experiment selected 785 Shaoxing duck groups (Hubei Shendan Healthy Breeding Co., Ltd.). % anticoagulant (2% Na 2 -EDTA, 1.6% NaCl, pH 8.0) in a centrifuge tube, record the wing number, extract genomic DNA, and store at -80°C.

[0020] According to the duck MTNR1C gene sequence (NCBI accession no.NW_004676748.1), the primer P-MTNR1C was designed, and the specific sequence is shown in SEQ ID NO.2 and SEQ ID NO.3.

[0021] Use the above primers to carry out PCR amplification in duck genomic DNA. The PCR reaction system is 25 μL, including 1 μL of DNA (50 ng / μL) template, 0.4 μL of upstream and downstream primers; 1.5 μL of dNTPs (10 mmol / L) mixture; 10× PCR buffer 2.5 μL; Mg 2+ (25mmol / L) 1.5μL; Taq DNase 0.25μL; ultrapure water 17.45μL. PCR amplification program: pre-denaturation at 95°C for 5 min; denaturation at 94°C for 30 s, annealing at 56.3°C for 30 s, extension at 72°C for 30 s, 35 ...

Embodiment 2

[0028] Association analysis of MTNR1C gene mutation site with age at first lay and egg production:

[0029] In a population of 785 Shaoxing ducks, the association analysis between different genotypes of MTNR1C gene and duck laying traits (including age at first lay, egg production at 34 weeks and egg production at 72 weeks) was analyzed. The PCR-direct sequencing method established in Example 1 was used to carry out individual genotyping, and the generalized linear model (GLM) of SPSS 18.0 software was used to estimate the relationship between egg production traits and genotypes by least squares method and test the significance of differences .

[0030] The results of the association analysis are shown in Table 2.

[0031] Table 2 Correlation analysis of MTNR1C gene 108C>T base mutation and duck laying performance

[0032]

[0033]

[0034] Note: Different letters a and b in the same column indicate significant difference (P<0.05); () indicates the number of individual...

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Abstract

The invention relates to a molecular marker relevant to the laying duck egg laying performance and application thereof in breeding, and belongs to the technical field of molecular markers. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1; the 108-th site position in the sequence has a C>T mutation; the site has the obvious relevance with the duck egg laying starting day age,the 34-week-age egg laying quantity and the 72-week-age egg laying quantity; the egg laying starting day age of the TT gene type is obviously lower than that of the CC and CT gene type; the 34-week-age egg laying quantity of the TT gene type is obviously higher than that of the CC gene type; the 72-week-age egg laying quantity of the TT gene type is obviously higher than that of the CC and CT gene type; through PCR amplification and product sequencing typing, the TT gene type duct can be selected. The molecular marker realizes the early stage breeding on the duck egg laying performance; the powerful help is provided for the work of the egg laying type duck breeding work.

Description

technical field [0001] The invention relates to a molecular marker related to the laying performance of laying ducks and its application in breeding, and belongs to the technical field of molecular markers. Background technique [0002] According to incomplete statistics, the annual stock of adult laying ducks in my country is about 200 million, the annual production of duck eggs is about 4 million tons, and the total output value of the laying duck industry exceeds 60 billion yuan. one. Especially in the past 20 years, China's laying duck breeding industry has developed rapidly. It is developing from decentralized breeding to moderate-scale operation. The degree of intensification is constantly improving, and industrialized operation is also developing. [0003] The egg production performance of laying ducks is affected by multiple factors such as genetics, environment, nutrition, and feeding management. Traditional breeding methods mainly select from the phenotype of egg p...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 曾涛卢立志唐建宏冯佩诗屈元启李国勤陈黎沈军达陶争荣田勇徐坚
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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