Molecular identification method of peanut hybrid

A molecular identification and hybrid technology, which is applied in the field of identification of peanut hybrids, can solve the problem of no relevant reports on the molecular identification of hybrid descendants of peanuts, and achieve the effect of accurate identification.

Active Publication Date: 2016-04-27
BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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Problems solved by technology

However, so far, there is no relevant report on the molecular identification of peanut hybrid offspring

Method used

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  • Molecular identification method of peanut hybrid
  • Molecular identification method of peanut hybrid
  • Molecular identification method of peanut hybrid

Examples

Experimental program
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Embodiment 1

[0021] Example 1: Luhua 14 (hereinafter abbreviated as LH14) × Tifrunner is the molecular identification method of the parent hybrid

[0022] 1. LH14×Tifrunner Peanut Simplified Genome Sequencing

[0023] Leaf materials of LH14 and Tifrunner peanuts were collected, DNA was extracted, and RAD sequencing was used for simplified genome sequencing.

[0024] 2. Development of LH14×Tifrunner peanut single nucleotide polymorphism

[0025] According to the results of RAD sequencing, search for SNP (single nucleotide polymorphism) molecular markers that can be used to distinguish LH14 and Tifrunner peanut varieties with HindⅢ enzyme digestion. Table 1 lists some of the SNPs (4 different sequences), of which The part of AAGYTT in bold is AAGTTT or AAGCTT. The sequence recognized by HindⅢ is AAGCTT, the sequence in Tifrunner is AAGCTT, which can be cut by HindⅢ, and the sequence variation in LH14 is AAGTTT which cannot be recognized by HindⅢ, so it can be used to distinguish LH14 and T...

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Abstract

The invention discloses a molecular identification method of a peanut hybrid. The method comprises the following steps: conducting reduced-representation genome sequencing on the male parent and female parent of the peanut hybrid, and analyzing a sequencing result so as to identify an SNP molecular marker between the male parent and the female parent and design a universal primer for the male parent and the female parent; and extracting the genome DNA of the peanut hybrid, and verifying whether the peanut hybrid simultaneously has the common characteristic segments of the male parent and the female parent through PCR reaction and enzyme digestion. By virtue of the method, the peanut hybrid can be rapidly and accurately identified.

Description

technical field [0001] The invention relates to a method for identifying peanut hybrids by means of molecular biology, and belongs to the field of biotechnology. Background technique [0002] China is the country that produces the most peanuts in the world, and peanut production plays an important role in the national economy. Peanut is an important oil crop and economic crop in my country, and it can also be used to extract important industrial raw materials. Peanut is also a traditional bulk export commodity in my country, as well as an important nutritional and health product, occupying an important position in the agricultural planting structure. [0003] Hybrid breeding is the most widely used and most effective peanut breeding method at home and abroad. Hybrid breeding is the use of different genotype parents for hybridization, through genetic recombination, so that the hybrid offspring will have different types of variation, and then through selective breeding to fo...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 李国卫万书波崔凤刘译阳徐国鑫韩燕孙秀芹
Owner BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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