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Molecular markers related to fiber diameter traits of sheep wool and their specific primer pairs and applications

A technology of wool fiber and molecular markers, which is applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., and can solve the problems of early seed selection and rapid seed selection, etc.

Active Publication Date: 2021-05-14
新疆畜牧科学院畜牧研究所 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a molecular marker related to the trait of sheep wool fiber diameter and its specific primer pair, and the specific primer pair is used as a preparation or kit for preparing in vitro detection of molecular markers related to the trait of sheep wool fiber diameter. Application, the application of the molecular markers in the selection of Chinese Merino sheep wool fiber diameter traits overcomes the above-mentioned deficiencies in the prior art, and it can effectively solve the existing process of breeding high-quality sheep with fine wool fiber diameter traits. , which has the problem of not being able to select early and quickly; the present invention discloses for the first time that the rs160836358 SNP site is applied to the selection of sheep wool fiber diameter traits

Method used

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  • Molecular markers related to fiber diameter traits of sheep wool and their specific primer pairs and applications
  • Molecular markers related to fiber diameter traits of sheep wool and their specific primer pairs and applications
  • Molecular markers related to fiber diameter traits of sheep wool and their specific primer pairs and applications

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Embodiment 1

[0022] Embodiment 1: The molecular marker related to the trait of sheep wool fiber diameter is located at the 30th exon of FAT3 gene chromosome 21 (1481091), and the nucleotide sequence of the 30th exon is as shown in the sequence table SEQ ID No.1 is shown.

Embodiment 2

[0023] Example 2: The pair of specific primers used to amplify molecular markers related to sheep wool fiber diameter traits includes two allele-specific primers (ASP1 / ASP2), one locus-specific primer (LSP) and A specific targeting amplification primer (STA), the sequence of an allele-specific primer (ASP1) is shown in the sequence table SEQ ID No.2, and the sequence of the other allele-specific primer (ASP2) is shown in the sequence The list of SEQ ID No.3 shows the sequence of the loci-specific primer shown in the sequence table SEQ ID No.4, and the sequence of the specific target amplification primer is shown in the sequence table SEQ ID No.5.

Embodiment 3

[0024] Embodiment 3: Reagent or preparation or kit of the specific primer pair described in embodiment 2.

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Abstract

The invention relates to the technical field of animal molecular markers, and relates to a molecular marker related to the diameter traits of sheep wool fibers and its specific primer pair and its application in the selection of Chinese Merino wool fiber diameter traits, which is related to the diameter traits of sheep wool fibers Molecular markers associated with traits, which are located at exon 30 of chromosome 21 of the FAT3 gene. The present invention discloses for the first time that the rs425144268 SNP site is used as a molecular marker of sheep wool fiber diameter, and discloses for the first time that the rs425144268 SNP site is applied to the selection of Chinese Merino sheep wool fiber diameter traits; When the molecular marker is used for the selection of good wool fiber diameter traits, it has the advantage of simple operation and can assist in the screening of sheep with small fiber diameter. Since it starts from the molecular marker detection level, it can improve the accuracy of breed selection and improve the detection efficiency.

Description

technical field [0001] The invention relates to the technical field of animal molecular markers, and relates to a molecular marker related to the trait of sheep wool fiber diameter and a pair of specific primers thereof. The application in preparation or kit, the application of the molecular marker in the selection of Chinese merino sheep wool fiber diameter traits. Background technique [0002] As the wool spinning industry at home and abroad continues to update the processing of wool and cloth art, the requirements for wool raw materials are more stringent, the product trend has been developing in the direction of lightness and softness, and a new milestone has appeared in the quality of wool in the world. The demand for superfine wool is gradually increasing. The wool yield and fiber fineness quality of fine-wool sheep are important indicators to determine its economic value, and the objective inspection of wool quality is getting more and more attention in wool sales, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 田可川黄锡霞赵冰茹田月珍付雪峰徐新明吴伟伟哈尼克孜张艳花石刚于丽娟何军敏杜建文阿米娜玛尔孜娅
Owner 新疆畜牧科学院畜牧研究所
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