Molecular marker relevant to reproductive performance of chicken and application of molecular marker in breeding

A molecular marker and reproductive performance technology, applied in the field of molecular genetics, to solve problems such as unclear mechanism and function
CN103789307AInactive Publication Date: 2014-05-14POULTRY INST SHANDONG ACADEMY OF AGRI SCI
1 Cites 4 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
POULTRY INST SHANDONG ACADEMY OF AGRI SCI
Publication Date
2014-05-14
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a molecular marker relevant to the reproductive performance of chicken and application of the molecular marker in breeding, belonging to the field of molecular genetics. The nucleotide sequence of the molecular marker is shown in SEQ ID No.1; a C->T base substitution exists at the 315th bp (base pair) of the sequence, thereby leading polymorphism of SspI-RFLP. The genome DNA of chicken is taken as a template, and primers shown in SEQ IDNo.2-3 are used for amplifying to obtain a PCR amplification product of 556bp; the PCR product is detected by using 1.5 percent agarose gel electrophoresis after being digested by an SspI restriction enzyme to obtain three banding patterns of CC, CT and TT; in local varieties with small egg weights and poor egg laying performance, CC genetype individuals are for reserved. A method for detecting the molecular marker relevant to the reproductive performance of chicken is simple and rapid and is not influenced by the environment, and early strain selection can be realized.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of molecular genetics, in particular to a molecular marker related to chicken reproductive performance and its application in breeding. Background technique

[0002] The main biological functions of bone morphogenetic protein receptor IB (BMPR-IB) gene include follicle growth and development, ovulation, bone tissue development, and organismal embryonic development. The study found that a single base mutation at the A746G (FecB) position of the BMPR-IB gene leads to multiple fecundity traits in sheep, and has been confirmed in multiple sheep breeds. Wang Shengbao's research shows that the litter size of individuals with A746G mutation BB genotype in Small-tailed Han sheep is significantly higher than that of the other two genotypes (P<0.01), and the litter size of heterozygous individuals in Sahan F1 generation is significantly higher than that of ++ type individuals (P<0.05). Yan Yadong's research shows that t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More