A Molecular Identification Method for Peanut Hybrids

Abstract

The invention discloses a molecular identification method of a peanut hybrid. The method comprises the following steps: conducting reduced-representation genome sequencing on the male parent and female parent of the peanut hybrid, and analyzing a sequencing result so as to identify an SNP molecular marker between the male parent and the female parent and design a universal primer for the male parent and the female parent; and extracting the genome DNA of the peanut hybrid, and verifying whether the peanut hybrid simultaneously has the common characteristic segments of the male parent and the female parent through PCR reaction and enzyme digestion. By virtue of the method, the peanut hybrid can be rapidly and accurately identified.

Description

technical field

[0001] The invention relates to a method for identifying peanut hybrids by means of molecular biology, and belongs to the field of biotechnology. Background technique

[0002] China is the country that produces the most peanuts in the world, and peanut production plays an important role in the national economy. Peanut is an important oil crop and economic crop in my country, and it can also be used to extract important industrial raw materials. Peanut is also a traditional bulk export commodity in my country, as well as an important nutritional and health product, occupying an important position in the agricultural planting structure.

[0003] Hybrid breeding is the most widely used and most effective peanut breeding method at home and abroad. Hybrid breeding is the use of different genotype parents for hybridization, through genetic recombination, so that the hybrid offspring will have different types of variation, and then through selective breeding to fo...

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