Method for identifying or assisting in identifying eye muscle area of 100-kg weight of pig and kit special for method

A technology for eye muscle area and identification of pigs, which is applied in the field of genetic engineering to achieve rapid identification

Active Publication Date: 2016-02-17
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

People's lives are constantly improving, and traditional fa

Method used

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  • Method for identifying or assisting in identifying eye muscle area of 100-kg weight of pig and kit special for method
  • Method for identifying or assisting in identifying eye muscle area of 100-kg weight of pig and kit special for method
  • Method for identifying or assisting in identifying eye muscle area of 100-kg weight of pig and kit special for method

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Embodiment 1

[0036] Embodiment 1. A method for identifying or assisting in identifying the eye muscle area of ​​pigs with a body weight of 100kg

[0037] 1. Screening of SNP sites of porcine H19 gene

[0038] 1. Extraction of DNA from pig ear tissue samples

[0039] The blood samples of 32 Landrace pigs from Hebei pig farms were collected respectively. After the samples were collected, they were stored in 75% alcohol and stored at -20°C. Genomic DNA of blood samples was extracted by phenol-chloroform extraction method to obtain genomic DNA of pig ear tissue. Specific steps are as follows:

[0040] (1) Preparation of ear tissue samples: Cut ear tissue samples of moderate size, wash away impurities and blood stains on the surface with normal saline, put them in a 1.5ml centrifuge tube, cut them into pieces with ophthalmic scissors, and add 500uL of tissue to it Lysis solution (Beijing Baitaike Biotechnology Co., Ltd.; product number: AU19011);

[0041] (2) According to the size of the cu...

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Abstract

The invention discloses a method for identifying or assisting in identifying the eye muscle area of the 100-kg weight of a pig and a kit special for the method. The method includes the steps of detecting whether the 16th-site deoxyribonucleotide of the H19 gene of the pig to be detected is C or T or C and T, determining whether the gene type of the pig to be detected is TT or CC or TC, and determining the eye muscle area of the 100-kg weight of the pig according to the gene type of the pig, wherein the eye muscle area of the 100-kg weight of the pig of the TT gene type is larger than that of the pig of the CT gene type, and the eye muscle area of the 100-kg weight of the pig of the CT gene type is larger than that of the pig of the CC gene type. It is proved through tests that by means of the method, the eye muscle area of the 100-kg weight of the pig can be accurately and rapidly identified, and important reference significance in genetic breeding and sexual ability testing of the pig is achieved.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a method for identifying or assisting in identifying the eye muscle area of ​​a 100kg pig and a special kit thereof. Background technique [0002] Pig is the first of the six livestock, and the pig industry occupies an important position in the world, especially in China's national economy. The proportion of pork in many meat products has been maintained at about one-third, and it is one of people's favorite non-staple foods. People's lives are constantly improving, and traditional breeding techniques can no longer meet people's demand for pork. With the development of modern breeding technology, the production performance of pigs has been significantly improved, and the healthy breeding and fattening of pigs is particularly important. [0003] Pig backfat thickness and eye muscle area are directly related to pig lean meat percentage, which are important index par...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李奎唐中林孙浩梁如意
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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