bPrimer batch PCR primer design method based on Primer 3

A primer design, batch technology, applied in computing, bioinformatics, special data processing applications, etc., can solve problems such as failure to prevent genetic diversity and primer design failures

Active Publication Date: 2016-06-29
HUNAN SHENGWEI GENE TECH
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a kind of bPrimer batch PCR primer design method based on Primer3, to solve the problem that the prior PCR

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • bPrimer batch PCR primer design method based on Primer 3
  • bPrimer batch PCR primer design method based on Primer 3
  • bPrimer batch PCR primer design method based on Primer 3

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0043] The method for designing targeted PCR primers in batches provided by the embodiments of the present invention solves the problem that the existing PCR primer design methods fail to design primers because genetic diversity is not prevented when designing PCR primers.

[0044] In order to enable those skilled in the art to better understand the technical solutions in the embodiments of the present invention, and to make the above-mentioned purposes, features and advantages of the embodiments of the present invention more obvious and understandable, the technical solutions in the embodiments of the present invention are described below in conjunction with the accompanying drawings The program is described in further detail.

[0045] Please refer to the attached figure 1 , with figure 1 A flow chart of the Primer3-based bPrimer batch PCR primer design method provided by the embodiment of the present invention is shown. From attached figure 1It can be seen that the bPrime...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a bPrimer batch PCR primer design method based on a Primer 3.The method includes the steps that an original sequence of a target DNA sequence is obtained; a high-frequency polymorphic locus in DNA polymorphic data is extracted; the high-frequency polymorphic locus is labeled; an annotated sequence of the labeled high-frequency polymorphic locus is output, and the annotated sequence and the original sequence are identical in basic group length; the annotated sequence is read to generate candidate primers; the candidate primers are screened.The bPrimer batch PCR primer design method based on the Primer 3 can avoid the high-frequency polymorphic locus, reduces amplification failure caused by genetic diversity of target population, can detect specificity of primers in batches, reduces amplification failure caused by nonspecific amplification, primer dimer and other reasons, can be used for evaluating specificity of existing primers, and can automatic segment long target segments.

Description

technical field [0001] The present invention relates to the technical field of PCR primer design, more specifically, relates to a bPrimer batch PCR primer design method based on Primer3. Background technique [0002] Polymerase Chain Reaction (PCR), as one of the most basic molecular biology experimental methods, can amplify the copy number of target DNA by millions of times in vitro, so it is widely used in genetic engineering, diagnostic reagents and other fields . [0003] A key factor for the success of PCR experiments is the design of PCR primers. Therefore, in order to design better primers, a wide variety of PCR primer design software has been invented. The earliest and most basic PCR primer design software is Primer0.5, and then Primer3, an open source PCR primer design software that is now widely used, is designed on the basis of Primer0.5. The most basic functions of Primer3 include designing PCR primers and designing hybridization probes, and it has the advantag...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G06F19/20
CPCG16B25/00
Inventor 戴立忠彭厘旻郭鑫武陈明王煜罗喜鹏
Owner HUNAN SHENGWEI GENE TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap