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Main effect QTL related to mung bean early flowering stage, molecular marker and application thereof

A technology of molecular markers and flowering stage, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of time-consuming and laborious identification of phenotypes, and weak genetic research, so as to reduce the workload of breeding , avoid time-consuming and labor-intensive, stable results

Active Publication Date: 2020-12-15
CROP INST ANHUI PROV ACAD OF AGRI SCI
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Problems solved by technology

[0004] In order to solve the problem of weak basic research on genes related to the initial flowering stage of mung bean, and the time-consuming and laborious problems that unlinked molecular markers can be used for marker-assisted breeding and phenotypic identification, the present invention provides a main effect QTL related to the initial flowering stage of mung bean, molecular markers and its application

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  • Main effect QTL related to mung bean early flowering stage, molecular marker and application thereof
  • Main effect QTL related to mung bean early flowering stage, molecular marker and application thereof
  • Main effect QTL related to mung bean early flowering stage, molecular marker and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] This example provides a major QTL related to the first flowering stage of mung bean. The major QTL locus is located between markers ID3-6 and ID3-7 on chromosome 3, and its physical location is 7195kb-9169kb. And, in F 2 In the population, the LOD value of the main effect QTL was 24.31, the additive effect value was 7.04, the dominant effect value was 1.02, and the contribution rate was 52.55%. Next, this embodiment describes the determination of the main QTL.

[0032]Firstly, construct mung bean segregation populations at flowering stage. The mung bean variety Weilu 11 with early flowering and purple stems was selected as the male parent, and the mung bean variety Sulu 16-10 with late-maturing green stems was used as the female parent to prepare a hybrid combination. The days of first flowering of Weilu 11 were 41 days, and the days of first flowering of Sulu 16-10 were 54 days. Because Sulu 16-10 has a late flowering period, it can be sown 15 days in advance. When...

Embodiment 2

[0039] This example provides a main effect QTL related to the first flowering stage of mung bean, and narrows the position of the main effect QTL qDFF3 in Example 1 to between the markers R3-15 and R3-19 of chromosome 3, and the physical position is 8504kb~ 8674kb, the LOD value is 61.17, the additive effect value is 8.73, the dominant effect value is 0.34, and the contribution rate is as high as 84.42%. In this example, on the basis of Example 1, the verification of the main QTL qDFF3 in flowering stage is completed and the QTL interval is further narrowed.

[0040] QTL identification usually requires years of phenotypic surveys and QTL analysis data. If a QTL can be detected multiple times, it is a stable genetic QTL locus, which has important breeding significance. In order to verify the main effect QTL qDFF3 of mung bean flowering stage, this example uses F 2 F produced by population selfing 3 Populations were subjected to phenotypic identification and QTL analysis. In ...

Embodiment 3

[0042] This embodiment provides a primer pair of molecular markers related to the mung bean flowering stage, which contains a pair of molecular markers; one of the molecular markers is closely linked to the main QTL related to the mung bean flowering stage in Example 2, and is located at the QTL on the left side, and is the InDel marker R3-15, which is located at 8504kb on the third chromosome, and the front and rear primer sequences are shown in the sequence table Seq ID NO.1 and the sequence table Seq ID NO.2; wherein another molecular marker is the same as that in Example 2 The main QTL related to the first flowering stage of mung bean is closely linked, located on the right side of the QTL, and is the InDel marker R3-19, located on the third chromosome 8674kb, the primer sequences before and after are shown in the sequence table Seq ID NO.3 and the sequence table Seq ID Shown in NO.4.

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Abstract

The invention discloses a main effect QTL related to a mung bean early flowering stage, a molecular marker and application thereof. The early flowering stage main effect QTL qDFF3 is located between InDel markers R3-15 and R3-19 on a third chromosome, the linkage marker R3-15 is located at 8504kb of the third chromosome, and the marker R3-19 is located at kb of the third chromosome. The two linkage markers are used for identifying the early flowering stage of mung bean resources, the phenotype and the markers show extremely significant correlation, the accuracy rate reaches 92.9%, and that thetwo markers can well identify the mung bean resource early flowering stage phenotype is indicated. The mung bean early flowering stage character is associated with the molecular marker, and the result is more stable and accurate. The two markers can be used for screening or identifying the mung bean resource early flowering stage, materials can be subjected to genetic typing in breeding low generations, the required early flowering stage phenotype materials are accurately screened, the breeding workload is reduced, and the selection efficiency is improved. On the other hand, fine positioningand gene cloning of the mung bean early flowering stage main effect QTL qDFF3 are facilitated, and the genetic regulation mechanism of the mung bean early flowering stage can be explained.

Description

technical field [0001] The present invention relates to a main effect QTL related to mung bean flowering stage in the technical field of mung bean variety resource genotype identification and breeding selection, in particular to a molecular marker related to mung bean flowering stage, and also to a mung bean flowering stage related Application of molecular markers in identification of mung bean resources on flowering stage. Background technique [0002] Mung bean is a cultivated species in the genus Vigna of the leguminous bean family. It is rich in nutrients and has a grain protein content as high as 19.5% to 33.1%, which is higher than that of rice, wheat, corn and other cereal crops. It is a high-protein, medium-starch, and low-fat food. . Not only that, mung beans are also rich in a variety of mineral elements, vitamins and active substances. They have the functions of detoxification, antibacterial and antibacterial, anti-allergic, lowering blood fat, lowering blood pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 叶卫军张阴王沛然杨勇田东丰周斌
Owner CROP INST ANHUI PROV ACAD OF AGRI SCI
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