S1, in the same time, the anti-white leaf blight gene XA7, XA21And anti-brownite gene BPH14, BPH15Material (HZ02414) is donor parent P2Together with anti-rice blast target gene pi1, Pita material (HZ02231) is donor parent P3Together with anti-rice blast target gene pi9And anti-white blanquull target gene XA23Material (HZ02455) is donor parent P4Excellent material to be reformally rendered by Huang Huang Huang Huang Huang Huang Huang Huang Huang Huangzhuzum is a receptor parent1And planting;
S2, will P2, P3, P4Pieces and P1Hybrid, get 3 f1Name A, B, C, each F, each F1The seed is above 50 capsules;
S3, planting S2 A, B, C seed and P1The SNP molecular marks (i.e., truthful hybrid) corresponding to the anti-pathogenic genotype are selected from the SNP molecules of the anti-pathogenic target gene, respectively, respectively, respectively, and the corresponding anti-pathogenic genotype1Pay back, get 3 BC1Fly1, Named D, E, F, each BC, respectively1Fly1The seeds are above more than 3,000 capsules;
S4, planting S3 D, E, F seed and P1The SNP molecule marks corresponding to the anti-pathogenic target gene is screened from D, E, F, respectively, a single plant corresponding to the anti-pathogenic genotype, and about 190 strains per group; from rice All genome SNP tags, separate P2, P3, P4With P1There is a polymorphic, evenly distributed from about 120 SNP markers on rice 12-chromosomes. The primary genome background selection of D, E, and F, respectively, with the corresponding approximately 120 polymorphic SNP tags, respectively, respectively, respectively, respectively, and P1The most similar strain background. Then use the D's selected single plant, harvest self-interaction F2More than 3,000 seeds, named G; polymerize hybridization of the neutralized single plant of E to f, obtain polymerization hybrid f1The seed is more than 1,000 capsules, named H;
S5, planting S4 G and H seeds and P1The corresponding anti-pathogenic genotype is hue according to the SNP marker of the anti-pest target gene closely chain from G. (XA)7Xa7Xa21 Xa21BPH14BPH14BPH15BPH158 monosplates, screening from H, corresponding anti-pathogenic genotype is heterozygous (Pi1pi1Pi9pi9Pitapitaxa23xa2348 monographs, then selected from the monograph of G and H.1In the phenotype of strain, the strain type, the venom period, the most similar forms of three, and the two were combined, and the three poly hybrids were obtained.1More than 1,000 seeds or more;
S6, planting S5 Trimer Hybrid F1Seeds, based on the SNP molecular marker of the anti-pest target gene, the SNP molecule, which is screened, corresponding to the eight anti-pests, and the target gene is heterozygous (Pi1pi1Pi9pi9Pitapitaxa23xa23Xa7xa7Xa21xa21BPH14BPH14BPH15BPH1548 single strains, screening and p1In the most similar single plant, the most similar single plant in the phenotype of the strain, the spike, and the growth period, each harvesting self-exchange f2The seeds are more than 2,000 capsules;
S7, planting S6 acquisition F2The screen is selected according to SNP markers closely chain with anti-pest target genes, and the total 8 target genes are selected and at least four target bases are screened when ripe, mature, maturation.1In the four monographs of four single strains in phenotypes such as high, ear type, and growth period, each harvesting self f3The seeds are more than 2,000 capsules;
S8, 4 F obtained by planting S73The plant is screened according to the SNP molecular marker of the anti-pathogenic target gene, and all 8 target genes are selected (Pi).1Pi1Pi9Pi9Pitapitaxa23Xa23Xa7Xa7Xa21Xa21BPH14BPH14BPH15BPH1516 strains of single strains, screening and p1In the most similar single plant, the most similar single plant in the phenotype of the strain, the spike, and the growth period, each harvesting self-exchange f4More than 1,000 seeds or more;
S9, 4 F obtained by planting S84The plant is screened, and one of the most uniform strains is screened, and the excellent single strain is screened and the self-cultivation seed is harvested.5More than 1,000 seeds or more;
S10, different strains obtained by planting S9, performing production identification, resistance identification, etc., the main advantages of Huang Huangzhang, but its rice blance, white-leaf and brown planthopper are significantly improved.
Defenseism identification, the anti-new germplasm is ≤3 to rice blast, white-leaf and brown planthopper resistance, which is significantly better than the original receptor Huang Huang Huangzhang.
The techniques used above the above test process are prior art, and will not be described herein.