Method for creating novel multi-resistance rice germplasm by using molecular marker

A technology of molecular markers and new germplasm, applied in the direction of botanical equipment and methods, applications, plant gene improvement, etc., can solve problems that have not yet been seen

Active Publication Date: 2021-05-18
HUAZHI RICE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there have been some research reports on the introduction of 2-4 resistance genes into rice by molecular marker-assisted selection technology, but it has not been seen that more tha

Method used

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  • Method for creating novel multi-resistance rice germplasm by using molecular marker

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[0027]Example 1

[0028]S1, in the same time, the anti-white leaf blight gene XA7, XA21And anti-brownite gene BPH14, BPH15Material (HZ02414) is donor parent P2Together with anti-rice blast target gene pi1, Pita material (HZ02231) is donor parent P3Together with anti-rice blast target gene pi9And anti-white blanquull target gene XA23Material (HZ02455) is donor parent P4Excellent material to be reformally rendered by Huang Huang Huang Huang Huang Huang Huang Huang Huang Huangzhuzum is a receptor parent1And planting;

[0029]S2, will P2, P3, P4Pieces and P1Hybrid, get 3 f1Name A, B, C, each F, each F1The seed is above 50 capsules;

[0030]S3, planting S2 A, B, C seed and P1The SNP molecular marks (i.e., truthful hybrid) corresponding to the anti-pathogenic genotype are selected from the SNP molecules of the anti-pathogenic target gene, respectively, respectively, respectively, and the corresponding anti-pathogenic genotype1Pay back, get 3 BC1Fly1, Named D, E, F, each BC, respectively1Fly1The se...

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Abstract

The invention provides a method for creating a novel multi-resistant rice germplasm by using a molecular marker. The method comprises the following steps: according to a breeding target, carrying out multi-gene pyramiding breeding on a parent material, wherein the breeding target at least comprises pyramiding bacterial blight resistance, brown planthopper resistance and rice blast resistance. According to the method, a molecular marker-assisted selection technology is combined with a conventional breeding technology, and the polymerization of eight disease and pest resistant genes such as rice blast resistant genes Pi1, Pi9 and Pita, bacterial blight resistant genes Xa7, Xa21 and Xa23 and brown planthopper resistant genes Bph14 and Bph15 is rapidly realized, so that the breeding time is greatly shortened, and the breeding efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for creating new germplasm of multi-resistant rice by using molecular markers. Background technique [0002] Rice plays an important role in food production. However, the problem of resistance to diseases and insect pests of rice is still very prominent at present. Most of the varieties or combinations that have passed the national examination and approval are not strong in resistance. [0003] The reasons for the above situation mainly include the following aspects: 1. At present, most breeding units adopt traditional breeding methods to breed resistance to diseases and insect pests, and the breeding efficiency is low; The germplasm carrying multiple known disease and pest resistance genes has low breeding efficiency. Therefore, the creation of rice parents or lines carrying multiple disease and insect resistance genes is of great significance to the disease and...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 吴云天冯凡石斌文叶昌荣李宙炜梁毅曹桂元肖金华彭俊华
Owner HUAZHI RICE BIO TECH CO LTD
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