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Method for creating novel multi-resistance rice germplasm by using molecular marker

A technology of molecular markers and new germplasm, applied in the direction of botanical equipment and methods, applications, plant gene improvement, etc., can solve problems that have not yet been seen

Active Publication Date: 2021-05-18
HUAZHI RICE BIO TECH CO LTD
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Problems solved by technology

At present, there have been some research reports on the introduction of 2-4 resistance genes into rice by molecular marker-assisted selection technology, but it has not been seen that more than 6 resistance genes have been aggregated into the materials to be improved for the three main diseases and insect pests at the same time. Report on Creation of Multi-resistance Rice Germplasm

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  • Method for creating novel multi-resistance rice germplasm by using molecular marker

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Embodiment 1

[0028] S1, in order to carry the bacterial blight resistance gene Xa at the same time 7 、Xa 21 and brown planthopper resistance gene Bph 14 、Bph 15 The material (HZ02414) is the donor parent P 2 , to carry the rice blast resistance target gene Pi 1 , Pita's material (HZ02231) is the donor parent P 3 , to carry the rice blast resistance target gene Pi 9 and bacterial blight resistance target gene Xa 23 The material (HZ02455) is the donor parent P 4 , using Huang Huazhan, an excellent material to be improved that does not contain these target genes for resistance to diseases and insect pests, as the recipient parent P 1 , and planted separately;

[0029] S2, the P 2 ,P 3 ,P 4 respectively with P 1 Hybrid, get 3 F's 1 , respectively named A, B, C, each F 1 More than 50 seeds;

[0030] S3, A, B, C seeds and P obtained by planting S2 1 According to the SNP molecular markers closely linked with the target gene of resistance to disease and insect pests, the individual...

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Abstract

The invention provides a method for creating a novel multi-resistant rice germplasm by using a molecular marker. The method comprises the following steps: according to a breeding target, carrying out multi-gene pyramiding breeding on a parent material, wherein the breeding target at least comprises pyramiding bacterial blight resistance, brown planthopper resistance and rice blast resistance. According to the method, a molecular marker-assisted selection technology is combined with a conventional breeding technology, and the polymerization of eight disease and pest resistant genes such as rice blast resistant genes Pi1, Pi9 and Pita, bacterial blight resistant genes Xa7, Xa21 and Xa23 and brown planthopper resistant genes Bph14 and Bph15 is rapidly realized, so that the breeding time is greatly shortened, and the breeding efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for creating new germplasm of multi-resistant rice by using molecular markers. Background technique [0002] Rice plays an important role in food production. However, the problem of resistance to diseases and insect pests of rice is still very prominent at present. Most of the varieties or combinations that have passed the national examination and approval are not strong in resistance. [0003] The reasons for the above situation mainly include the following aspects: 1. At present, most breeding units adopt traditional breeding methods to breed resistance to diseases and insect pests, and the breeding efficiency is low; The germplasm carrying multiple known disease and pest resistance genes has low breeding efficiency. Therefore, the creation of rice parents or lines carrying multiple disease and insect resistance genes is of great significance to the disease and...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 吴云天冯凡石斌文叶昌荣李宙炜梁毅曹桂元肖金华彭俊华
Owner HUAZHI RICE BIO TECH CO LTD
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